Abstract:Thyroid hormone, mainly active triiodothyronine (T3), plays important roles during the fish post-embryo development including metamorphosis. Because of very small amount of T3 in whole body of larva, its distribution in different tissues during fish post-embryo development is not easy to be detected. As one kind of deiodinases, deiodinase I can convert thyroxine (T4) into the active T3. The expression pattern of Dio1 gene during post-embryo development can indirectly indicate the T3 distribution in different tissues. Dio1 gene is mainly expressed in intestine, gill, liver, body muscle, fin, and skin around eyes in post-embryo developing flounder Paralichthys olivaceus, indicating T3 regulates those tissues development. Specially during the process of anal fin and dorsal fin (including crown-like larval fin) development, expression pattern of Dio1 was evidently associated with the formation of fin fold, fin rays and pterygiophores, suggesting thyroid hormone plays important role in regulating media fin development.

Abstract:Growth performance of F₂ of 5 specialized strains of Macrobrachium rosenbergii(A,B,C,D and E) was checked by one-way completely randomized design and polyculture in concrete ponds with the fluorescent tag. The results indicated that the ranks of AGR and SGR were D>B>E>C>A in either one-way completely randomized experiment or polyculture in concrete ponds with the fluorescent tag. AGR and SGR by ANOVA and Duncan for strains of multi-average revealed that F₂ of B and D were strains with the best growth traits.Every proportion of body form of F₂ was greater than F₁; Variation coefficient of body weight of F₂ was lower than F₁, the most obvious decline in the comparison of F₂ and F₁ was B, decreasing 49.22%.All of them suggested preliminary benefit of strain selecting.Microsatellite markers were used to analyze the genetic diversity and genetic differentiation of F₂ of 5 specialized strains, including 630 individuals. The results showed the allele number was 6.25; average effective allele number was 3.666 2; average observed heterozygosity value was 0.435 0; the average expected heterozygosity value was 0.707 6. All of these five specialized strains in the ascending order the PIC was C(0.434 0)< A(0.480 4)

Abstract:In this study, we cloned a novel partial cDNA of toll-like receptor(TLR) gene from total RNA of gill of living giant freshwater prawn (Macrobrachium rosenbergii) of Shanghai population by RT-PCR by using primers designed from the partial sequence from transcriptone sequencing result, then it was sequenced and bioinformatically analyzed. Sequence analysis showed that the part of TLR cDNA was 1 875 bp in length and the open reading frame encoded a deduced protein with 575 amino acids residues. The part of TLR is a transmembrane protein that contains several domains like LRR,LRR-CT, LRR-NT motif in the extracellular domain and a TIR domain in the intracellular region, the common structural features of TLRs. The results of the amino acids sequence of TIR were compared with other species, indicating a highly conserved similarity among Litopenaeus vannamei,Marsupenaeus japonicus,Fenneropenaeus chinensis,Penaeus monodon,Eriocheir sinensi and Scylla paramamosian. Besides, similar RF PCR products of TLE gene were also amplified from total RNA of other different tissues including haemocytes, hepatopancreas, muscle and androgenic gland.

Abstract:Polymorphic microsatellite markers were developed from black rockfish(Sebastes schlegelii) with an enriched partial genomic library by magnetic beads and the polymorphisms of these loci were assessed in 32 individuals from a wild population. We screened 58 positive clones by three-primers PCR method and sequenced them, then 47 sequences were detected containing microsatellites, and 15 pairs of polymorphic microsatellite primers were obtained. A total of 66 alleles were detected, with the number of alleles ranging from 2 to 14 (averaging 4.4). The observed and expected heterozygosity values ranged from 0.062 5 to 0.968 8 and 0.061 5 to 0.929 1, respectively. The values of polymorphism information content indicated, six loci were highly or moderately polymorphic. HJ2-32,HJ4-27,HJ4-45 and HJ4-47 significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found. The result demonstrates these markers will facilitate relevant research in S. schlegelii.

Abstract:We analysed the sequences of three mitochondrial DNA (COⅠ), 16S rDNA, cytochrome oxidase b) and nuclear gene Tmo-4c4 to identify the phylogenetic relationship among the grouper hybrids and their parents (Epinephelus moara ♀ and Epinephelus akaara ♂; Epinephelus moara ♂ and Epinephelus akaara ♀). Among the four sequence fragments there was insertion deletion 16S rDNA but no insertion deletion in other three gene fragments which all belong to coding gene. As shown in results, in 12 sequences there were 4 haplotypes and 41 nucleotide polymorphic loci in COⅠ gene fragments (387 bp), 2 haplotypes and 21 nucleotide polymorphic loci in 16S rDNA gene fragments (529 bp), 2 haplotypes and 49 nucleotide polymorphic loci in Cyt b gene fragments (383 bp), and 6 haplotypes and 8 nucleotide polymorphic loci in 16S rDNA gene fragments (467 bp). Sequence divergences and genetic distance analysis showed that three mitochondrial gene sequences in the two crossing hybridized generations all showed 100% similarities respectively with the fragments of the female parents, but there were only 90% and 89.4% similarity in COⅠ, 96% and 96% similarity in 16S rDNA and 87% and 87.2% similarity in Cyt b with their male parents. In nuclear gene Tmo-4c4 there were 98.9% to 99.6% similarity with their female parents and 98.7% to 99.4% similarity with their male parents. The results indicated that there was no remarkable genetic difference. All above results imply that the two crossing grouper hybrids inherited from their female parents in three mitochondrial genes and there is no remarkable genetic difference in nuclear gene Tmo-4c4.

Abstract:In this study, observation on molting and growth of Chinese mitten crab (Eriocheir sinensis) at adult stage (200 days) was carried out under laboratory conditions. The results showed that the average growth rate of body weight at the first to fourth molting stages was 48.7%, 69.1%, 80.1%, 64.8%, respectively; the average carapace length growth rate was 21.3%, 17.0%, 29.5%, 16.7%, respectively; the average carapace width growth rate was 17.4%, 18.3%, 28.2%, 18.1%, respectively. The body weight, carapace length and carapace width of male individuals were higher than those of female individuals at each molting stage, and the significant differences (P<0.05) were found since the third molting time. The average molting period was 37 days, 38 days, 29 days and 41 days for the first to fourth molting stages, respectively. The coefficient of variation of growth was higher at the first two molting stages than that at the latter two molting stages. The initial weight had significant effect (P<0.05) on the weight gained after each molting. There was significant difference (P<0.01) in weight,carapace length and carapace width among each molting stage, and the body weight after the third molting stage had significant correlation (P<0.05) with the weight at other molting stages. This result also showed the carapace length and carapace width had significant correlation(P<0.05) with the culturing temperature, however, the body weight had slightly significant difference (r=0.917-0.923, 0.05<P<0.10) with the culturing temperature. Generally, the present study showed that the growth rate was the highest at the third molting stage, indicating its important effect on the growth and culture practice at this stage. Meanwhile, the results showed that the larger size of juveniles, the larger size of adults would be.

Abstract:To determine the effects of rice-crab culture systems on water environment and rice yield in paddy fields, periodic sampling was conducted in rice-crab culture systems with different crab densities (18 ind/m², low density (LD); 54 ind/m², high density (HD)) and non-crab paddy field (CK) at six key growth stages (seeding stage, reviving stage, tillering stage, elongation stage, flowering stage, filling stage). The samples were analyzed for water quality and rice yield. The results revealed that the dissolved oxygen concentration of CK was higher than that in stocking crab paddy field in the rice-growing cycle. It was significantly different(P<0.05) between CK and stocking crab paddy field, when the rice was only in flowering stage. There was no significant difference (P>0.05) between LD and HD. At tillering stage, the ammonia nitrogen concentration of HD was significantly higher than CK (P<0.05). At filling stage, the total phosphorus (TP) concentration of CK was the highest, and it was significantly different (P<0.05) between CK and stocking crab paddy field. It was not significantly different (P>0.05) between LD and HD. It was lack of phosphate fertilizer from the beginning of tillering stage. It was lack of nitrogen fertilizer from the beginning of flowering stage and filling stage. It was appropriate to stock larval crab in paddy field, when rice was in tillering stage. Because concentration of nitrite and ammonia nitrogen decreased rapidly below the crab safe concentration. The rice yield of LD was significantly higher than that in CK and HD (P<0.05). The rice yield of CK was the lowest, and it was significantly different (P<0.05) between CK and HD.

Abstract:In order to investigate the feeding habits and growth of Triplophysa (Hedinichthys) yarkandensis (Day) in highly saline-alkaline water, this study examined and analyzed 237 T.(H.) yarkandensis (Day), aquatic organisms and water quality based on 3 water areas of the Tarim River Alar, channel and Tainan River from 2012 to 2013 through fish biology, fisheries resources survey and other research methods. Results showed that the T.(H.) yarkandensis (Day) has relatively wider mouth, lower mouth position, and similar characteristics in feeding organs and intestines compared with other Triplophysa; the proportion of length body and length intestinal was 1:1 belonging to omnivore which prefers to feeding on other animal; the aquatic organisms and water qualities are quite different in the 3 water areas, and there is no the sufficient diet for T.(H.) yarkandensis (Day); its food composition simplified and feeding intensity decreased; cannibalism, low survival rate and growing instability of T.(H.) yarkandensis (Day) are founded as well. In conclusion, since its feeding morphology did not change but food composition changed responding to different waters, T.(H.) yarkandensis (Day)'s behavior may change for adjusting to these feeding behaviors for the long term run.

Abstract:The early development characteristics of Tanakia chii larvae and juveniles were observed on the artificially fertilized and hatched fish. Newly hatched larva contained a substantial amount of yolk, with pale yellow in color and tiny tubercles on the surface. On day 3, a pair of hilly projections of yolk expanded toward both sides of the body, and the primordial fin-fold at the caudal portion developed. Optic cups developed into circular on day 6. Lens and melanin pigments began to form on the optic cups on day 8. On day 11, the mouth formed and brain developed, and melanophores were observed on the body. On 15 days old larvae, the branchial groove and anus had developed, the base of dorsal fin and anal fin formed, and caudal fin rays formed at the lower lobe. On day 21, the boundary of the head and trunk was clear, the gas-bladder divided into anterior and posterior lobes. The numbers of the dorsal fins and anal fins were 8 and 10 respectively. Even though the larva still had partial yolk-sac left, it started to eat to replenish energy, and the larvae began free swimming since then. During day 31 to day 36, rudiments of the pectoral fin appeared as small membranes, and some rays were formed in the pelvic fin. The caudal fin changed from a truncated to a deep forked shape. In 61 -63 days old juvenile, scales appeared on the body and a black longitudinal line appeared on the middle and latter parts of body. Since then, the fish always swam as a cohort. The growth curve of body length could divide into two stages: the growth rate was relatively slow from 1 to 21 days after hatching, and the growth rate was higher from 21 to 81 days old. We consider day 21 might be the age when Tanakia chii swims out of the mussels because we observed the larvae began to eat at that time. Furthermore, development of sensing organ, feeding, breathing and swimming apparatus is rapid in the early life stage of Tanakia chii, which may be important in providing the larvae with ability to cope with external environment and improve their survival rate after the larvae swim out the host mussel.

Abstract:In the present study, three multivariate morphometrics analyses were introduced to assess the morphological variations on meristic parameters,measurable scaling parameters and truss network scaling parameters in Parabramis pekinensis, Megalobrama amblycephala, M. terminalis (Dongjiang population and Qiantangjiang population), M. pellegrini and M. hoffmanni in China. Results were shown below: No significant variation(P>0.05)was observed on seven meristic parameters such as scale number on lateral line, scale number above lateral line, scale number beneath lateral line, soft dorsal fin (spines, rays), pectoral fin (spines, rays), pelvic fin (spines, rays), anal fin (spines, rays) among Parabramis and Megalobrama species. Based on nine measurable scaling parameters and twenty truss network scaling parameters, cluster analysis presented a relatively close relationship between M. amblycephala, M. terminalis and M. pellegrini, and these three species had relatively far relationship with the species of genus Parabramis, while they all had great morphological variation with the species M. hoffmanni. Meanwhile, certain morphological variations were detected between Dongjiang population and Qiantangjiang population of the species M. terminalis. Discriminant analysis outputted six discriminant functions with a comprehensive accuracy rate of 93.7% among the six populations of genera Parabramis and Megalobrama. The cumulative contributive proportion of two chief principal components extracted by principal component analysis was 77.9%, in which principal component 1 accounted for 70.9%. Characters that played key roles in inter-species morphological variation among Parabramis and Megalobrama concentrated mainly in the direction of dorsal-ventral axis.

Abstract:Xiao Yangshan is located on the north of Yangshan port, which is the first offshore deepwater port in China. Phytoplankton community structure of Xiao Yangshan sea area was investigated and analyzed from January to December in 2012. A total of 183 phytoplankton species, belonging to 7 phyla and 77 genera, were identified. Diatom, including 51 genera and 143 species which accounted for 78.14% of species richness, was the main phytoplankton group.The annual average cell abundance of phytoplankton was (7.01±6.26)×10⁴ cell/L. Skeletonema sp. and Paralia sulcata were the main dominant species through the year, while Thalassiosira leptopus, Nitzschia frustulum, Rhaphoneis rhomoides and Cyclotella meneghiniana were the common species. Based on analysis of diversity, the annual average values of Shannon-Wiener index, Margalef index and Pielous index were 2.59±0.75, 4.00±1.44 and 0.55±0.15, respectively. The average number of Diatoms Index was 1.06±0.20. Redundancy analysis (RDA) showed that salinity, water temperature, dissolved oxygen and suspended solids concentration were the main environmental factors with great influences on phytoplankton.

Abstract:As the biggest local lake, Dianshan Lake plays an important role in fishery, aquatic food supply and conservation for aquatic living resources for Shanghai. In this study, we analyzed the annual variations on diversity of fish community and its status of this lake based on the survey data in Dianshan Lake from 2010 to 2012. The results showed that fish species composition was similar among the three years and the dominant species tended to be mini type fish of fish composition. It indicated that the Diversity Indices of fish community in Dianshan Lake were not significantly different among the three years (P>0.05). Moreover, the analysis of k dominance curves showed that the fish community was disturbed in Dianshan Lake in the three years. It is necessary to adjust management rules to maintain fisheries resources by improving water quality, relieving the fishing intensity, and improving fish stock enhancement in this lake.

Abstract:Green tide outbreak results in massive Ulva prolifera accumulation in coast China. Because Ulva prolifera has high proportion of cellulose content, it can be used as the raw material for manufacturing bio-ethanol when cellulose degradation produces oligosaccharides. In this study, by using Congo red staining method, eight larger lysis zone strains which have the ability of cellulose-decomposing were obtained from rotted Ulva prolifera and green tide outbreak sea area. Cellulose enzyme activity characteristics and the ability to decompose Ulva prolifera of each strain were studied and high cellulose enzyme activity strains were identified. Enzyme activity of FPA and CMC in strains H3, H4, H6 and Q1 was higher than others, and carboxymethylcellulose as carbon source induced enzyme work best. Enzyme activity of CMC in the strain H3 was the highest (56.98 U/mL). And the best reducing sugar yield were 10.4% and 12.8% from strain H3 and strain H4. All the four strains were obtained by morphological identification and molecular biology identification.The experiments show that Ulva prolifera can be used as a raw material for preparation of bio-ethanol, and the screened strains can used for cellulose degradation.

Abstract:This study was designed to measure the changes of the carotenoid content in tissues(skin, fin, muscle, eyeball, gill)of pink, red spots and black spots red tilapia, and thus to infer carotenoid expression and sedimentary method between different tissues by adding different amounts of astaxanthin in the feed. The results show that when the dosage of dietary astaxanthin is 400 mg/kg,the carotenoid content in tissues of red tilapia was significantly increased and the body color of red tilapia was significantly improved compared with the control group, while the carotenoid sedimentary amount in the gill of dark spots red tilapia has improved significantly compared to the control group (P<0.05). When the dosage of dietary astaxanthin is 800 mg/kg,the carotenoid sedimentary amount in the fin of pink red tilapia and the gill of dark spots red tilapia has improved significantly compared to the control group (P<0.05),but the other tissues of the carotenoid content of other tissues showed a downward trend compared to the control group and the dosage of 400 mg/kg, and the body color of the red tilapia did not change significantly. Astaxanthin content has a significant impact on the total carotenoid content of red tilapia tissues (P<0.01). The impact of the body color of red tilapia on total carotenoid content was not significant (P>0.05). The interaction between astaxanthin and body color has a more significant effect on carotenoid content (P<0.05). Cluster analysis showed that deposition rate in fin is the highest, followed by gill, and the next is the eyeball, deposition rate in skin and muscle is the lowest.

Abstract:We studied the effects of anti-infection of white spot syndrome virus (WSSV) by adding different concentrations of astragalus polysaccharides (APS) to the basal diet in Procambarus clarkii. The healthy P.clarkii which were negative of WSSV by PCR detection were injected intraperitoneally, with 100 μL WSSV, and fed with 0%, 0.2%, 0.4%, 0.8% of APS bait. After feeding 20 d, the result revealed that when the positive control group mortality rate was 100±0%, the mortality of 0.2%, 0.4%, 0.8 % APS addition groups were 86.67%±13.33%, 91.11%±7.70%, 73.33%±17.64%,respectively. In order to evaluate the effect of APS on visceral organs, such as gill, hepatopancreas, and myocardial tissue in P. clarkii, the tissue sections were observed. The results showed that there was significant pathological change like nuclear pyknosis, cell ruptured and arranged desultorily in the group of 0% APS bait, but there were no obviously pathological changes in the gills and hepatopancreas, whereas nucleoli displayed a certain degree of shrinkage, while the cells have not yet broken in the myocardial tissue in the group of adding 0.8% APS bait. This experiment showed that the survival rate can be increased to 26.67% when adding 0.8% APS bait compared with the positive control in P.clarkii. The APS can significantly improve the effect of resistance of P. clarkii to the WSSV infection, and it can be expected to receive good economic benefits in the actual aquaculture production.

Abstract:Drifting fish aggregation devices (FAD) are used widely to aggregate and catch tropical tunas in the purse seine fishery. Dolphinfish (Coryphaena hippurus) is one of the common by-catch species in purse seine fishing with FADs. The study of their biological characteristics is helpful to better understand the population structure, and has important significance on the reasonable resource conservation. The biological characteristics (length distribution, sex ratio, sexual maturity and diet) of 189 individuals are analyzed using the fishery biological data collected by the scientific observers onboard purse seine fishing vessels operating in the Western and Central Pacific Ocean (5°N-10°S, 145°E-170°E) from 2010 to 2013. The results indicated (1) fork length of dolphinfish associated with drifting FADs ranged from 10.0 to 126.3 cm, with the dominant fork length of 70.0 to 100.0 cm, accounting for 66.7% of the total; (2) sex ratio is about 4.1:1, and a significant deviation (χ² =68.17, P<0.05) from the expected 1：1 female to male can be observed; (3) the mature individuals (Ⅴ-Ⅵ) were dominant under the FADs, accounted for 73.6%, and the estimated size at 50% maturity is 49.95 cm; (4) 76.0% of stomachs are empty, but some commonly small associated fish species, such as Katsuwonus pelamis, Decapterus macarellus, Caranx sexfasciatus, Canthidermis maculate, and Aluterus monoceros are found in the stomach of dolphinfish. The information of fishery biology suggests the mature and large individuals are dominant in the dolphinfish school associated with drifting FADs, which have a stronger aggregation effect on females, and feeding is one of the possible potential motivations for dolphinfish aggregating around the FADs.

Abstract:Yellow catfish (Pelteobagrus nitidus)is an important target species in Dianshan Lake fishery. From August 2011 to July 2012, 780 specimens of Pelteobagrus nitidus were sampled to study their biology.The results showed that: (1) The length distribution of yellow catfish samples was 4.7-16.0 cm, with a dominant group of 8.0-12.0 cm; The weight distribution of those samples was 1.3-28.4 g, with a dominant group of 5.0-15.0 g. (2) The mean length of females and males were 10.93 cm and 11.37 cm, with a mean weight of 15.1 g and 15.88 g, respectively. (3)The length-weight relationships of females and males were described as W_♀=0.041×L^2.533, and W_♂=0.037×L^2.557, respectively, and with significant difference based on covariance analysis. (4) The length of minimum sexual maturity of female and male yellow catfish were 8.6 cm and 9.4 cm respectively. (5) The mature individuals' gonad somatic index (GSI) of yellow catfish was obviously higher than the immature individuals' and the female immature individuals' GSI,which tended to increase with total length and conversely for the female mature individuals'. (6) The main prey of yellow catfish was shrimp and the feeding intensity was high in the classes 2 and 3 which accounted for 57.66% of the total specimens.

Abstract:Based on the 539 samples of Sthenoteuthis oualaniensis collected by Chinese squid jig fishing fleets during April to June of 2013 in the high seas nearby the equator, the biological characteristics were studied. The result indicated that the mantle length (ML) ranged from 137 mm to 338 mm with a high concentration in 160 mm to 220 mm accounting for about 81.45% of all the samples, and the body weight (BW) ranged from 95 g to 1 300 g with a dominative weight from 150 g to 452 g accounting for about 80.76%, respectively. The composition of ML and BW was different among different months, and the dominative ML and BW range increased with the month increase. The ratio of the somatic body weight (SBW) to BW ranged from 40.57% to 87.76% with an average of 59.43%. The relationship between the ML to BW and ML to SBW was best described by the power function and exponential function by the Akaike's information criterion (AIC), respectively. The ratio of females to males was 76:1, and the samples were mostly composed of maturity individuals accounting for about 68.83% of all the samples.However, the maturity stage differed from month and increased with the month increase. The feeding stage was mainly in 0, 1 and 2 grades accounting for 89.42% of all the samples and the food was mainly composed of squids, crustacean and immature fishes.

Abstract:Based on the 46 samples of Sardinella aurita and 53 samples of Sardina pilchardus taken from the Chinese trawling vessel working in the Morocco coastal waters from June to August in 2011, their otolith morphology was analyzed based on traditional measurement and Fourier analysis. Six of variables were measured on the otolith and differences were compared by t-test; digitalized photographs of otolith were transformed to Ellipse Fourier descriptors (EFDs). Finally, six variables and 77 standardized EFDs were analyzed by principal component analysis (PCA) and stepwise discriminant analysis (SDA). The results showed that the total length (TL), total width (TW), dorsal width (DW), and ventral length (VL) had significant differences between two species (P<0.01), whereas dorsal length (DL) and wing length (WL) had no difference (P>0.05). Result of PCA showed that the first and second components made total contribution of mutation for 77.0% by traditional measurement, and the highest variables were total length/fork length (TL/FL) and dorsal width/fork length (DW/FL) respectively, the scatter-plot reveal a good dispersion; there were 16 principal components with 82.8% cumulative contribution by Fourier analysis, but the first two components had too much overlap with a bad separation. SDA showed that three variables of traditional measurement entered in the SDA, total correct classification was 83.76%; 9 harmonics of 77 EFDs entered in SDA with Fourier analysis, and the total correct classification was 92.02%. Overall, Fourier analysis is more effective than traditional measurement in the fish otolith morphology classification.

Abstract:Based on the survey from September 21, 2012 through November 15, 2012 in the waters near Cook Islands by Chinese tuna longliner, we analyzed the biological characteristics of albacore tuna, such as fork length distribution, fat content distribution, the temporal and spatial distribution of fat content, and the relationship between fat content and fork length. The results showed: (1) The fork length of the albacore tuna was 0.50-1.14 m, the average fork length was 0.95 m, and dominant with 0.90-0.99 m, accounted for 80.36%; (2) The minimum fat content of albacore tuna was 0.3%, the maximum fat content was 4.6%, and the average fat content was 2.0%, and the size composition was very rich; (3) From September 21 to October 13, 2012, the fat content of albacore tuna was low, while after October 13, 2012, the fat content of albacore tuna increased over time; (4) In the northwest area (11°S-13°S,162°W-168°W), the fat content of albacore tuna (2.7%-3.0%) was higher than that of the other areas (1.0%-2.5%); (5) There was no significant relationship between fat content of albacore tuna and its fork length(0.85-1.14 m, P>0.05). This study suggested: (1) After mid-October, the albacore tuna started to store fat to prepare for spawning; (2) The albacore tuna captured in the survey were mature individuals, basically, and they began to spawn and were migrating from the southern waters to northern tropical waters; (3) The spawning might occur in the northwest area (11°S-13°S, 162°W-168°W) of the waters near Cook Islands.

Abstract:Dongying City is the core region of Yellow River Delta Efficient and Ecological Economic Zone. Land salinization in this area is so serious that it has become the dominant factor that restricts the sustainable development of agriculture. Therefore developing fishery is an important way to reuse saline land, improve ecological environment, raise living standards and promote the transformation of resource-based city in Dongying. Five efficient ecology fishery models are put forward by means of positivist study on differences of ecological environment and embryos of eco-agriculture in Dongying. They are marine capture fisheries and fishery stock enhancement and releasing, sea cucumber aquaculture, crab aquaculture, shellfish culture and recreational fishery. The total yields, value of aquatic products and net income of fishermen have increased 9.5 times, 16.2 times, 13.9 times respectively during 1992 to 2011. Meanwhile, 25 136 hectares of saline land have been reused by fishery, which has made significant ecological, economic and social performance. Various methods are applied to assess the benefits of these models. The corresponding suggestions are proposed to optimize the fishery industry in Dongying City.

Abstract:As a kind of carnivorous fish, largemouth bass is of high economic value. When it is farmed, its food habit should be transformed from the living food into artificial diet. The traditional way of feeding domesticating has such problems as low survival rate, cannibalism and heavy workload. To solve these problems, on the basis of the food characteristics of largemouth bass, a device depending on waterflow was developed. Furthermore, the device was optimized under the guidance of the hydrodynamics calculation. The diet is taken into pond by the water current and the diet is evenly distributed on the water surface. In this way, the bass larvae grow at a similar speed, which alleviates the problem that the bigger larvae tend to eat the smaller ones. At the same time, this device can realize automatic feeding in fixed time and places, which is favorable to domesticating the larvae, and reducing labor intensity. Compared with the traditional way of farming, using the device to domesticate larvae will increase the survival rate and reduce the workload, thus significantly improve the economic benefit of farming.

Abstract:Two-buoy wave energy device is a form of oscillating float technology, and it is of great significance to study the power conversion characteristics for development of wave energy devices. This paper established the device's dual-degree of freedom forced vibration equations based on linear wave theory and viscous damping theory, and expression of optimal absorbed power of the device was deduced under condition of the optimum damping. Added masses coefficients, damping coefficients and wave excitation force were calculated by using HydroStar software, then, absorbed power and capture width ratio were obtained. The paper also analyzed effects on power absorption characteristics with the change of geometry parameters of two-buoy wave energy device in actual sea conditions.