XU Gang-chun , DONG Jing-jing , NIE Zhi-juan , XU Pao , GU Ruo-bo
2012, 21(4):481-488.
Abstract:The aim of this study was to provide technical parameters for the foundation of genetic background and resources in Coilia nasus.In the present study, lactate dehydrogenase isozymes (LDH) in liver, eye, kidney, muscle, gill and serum, and DNA content in tail fin, gill, muscle, gonad and liver were studied by isozyme and flow cytometry techniques. The results indicated that: (1) The LDH in different tissues had a tissue specialty. All the tissues exhibited five isozyme bands and same relative migration rate, except for LDH c expression. No LDH2 bands were found in muscle and serum. (2) No significant differences were observed in DNA content in gill, muscle and liver (P>0.05), but DNA content in those tissues was significantly lower than that in the tail fin and ovary cells (P<0.05). The ratio of DNA content in gill, muscle and liver and haploid spermatide were 2.16,2.19 and 2.22, respectively. Moreover, the hepatocytes which were easily isolated from tissue and made into single cell suspension were good materials for ploidy analysis by flow cytometry.
GE Hui-zheng , JIA Zhi-ying , LI Chi-tao , BAI Ying-ying , WU Yao , SHI Lian-yu
2012, 21(4):489-494.
Abstract:A set of 8 microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp♀×German mirror carp♂, and inverse cross group of German mirror carp♀×Heilongjiang carp♂. The results showed that: (1) observed heterozygosity values of parents and offspring were 0.607 1, 0.714 3, 0.681 8 and 0.708 3, 0.538 6, 0.743 8, and polymorphism information content were 0.442 1, 0.281 3, 0.553 5 and 0.673 5, 0.375 9, 0.624 1 in two groups, indicating that all 8 microsatellite loci showed polymorphism. (2) In the obverse cross group, the accumulative exclusion rate using 8 microsatellite loci was 99.92% and the appraisal ability was 98.00% while confidence was 95%. In the inverse cross group, the accumulative exclusion rate was 99.99% and the appraisal ability was 99.00%. (3) about relation between microsatellite markers and appraisal ability, we compared the accumulative exclusion rates and the appraisal ability using 3, 4, 5, 6, 7, 8 microsatellite markers. 6 and 8 high polymorphism markers were successfully applied in paternity determination of two cross groups.
WEI You-chuan , HUAN Rong-jun , LU Zhuan-ling , CHEN Dai-jian , JI Yong-jie , CHENG Guang-ping
2012, 21(4):495-501.
Abstract:The full cDNA sequence of hepcidin in grass carp Ctenopharyngodon idellus has been isolated by using RT PCR and RACE method. The hepcidin consists of 774 bp, including an 282 bp open reading frame (ORF), a 117 bp 5′-UTR and a 383 bp 3′-UTR . The 3′-UTR contains a polyadenylation tailed signal (AATAAA) and 1 mRNA instable motifs (ATTTA). The deduced hepcidin protein comprised 93 amino acid residuces with a signal peptide predicded by using SignalP 4.0 program. Putative amino acid sequence of hepcidin shared 27.9%-51.6% identity with its counterparts from other fish species. Phylogenetic tree constructed by using neighbor joining method revealed that prohepcidin was clustered with that from other teleost fish reported previously. The transcription of hepcidin was examined by quantitative real time PCR, and its mRNA was mainly expressed in liver, spleen and head kidney, and increased significantly in liver, spleen and head kidney between 4 h and 48 h after injection with Flavobacterium columnare.
LIU Li , LIU Peng , JIN Jia-li , WU Hai-li , WANG Gai-gai , JIANG Chen , CAI Ke-jun
2012, 21(4):502-508.
Abstract:Mx protein is one of the classes of antiviral proteins induced by type I interferon (IFN).A full length cDNA sequence of Mx gene from grass carp (Ctenopharyngodon indellus) was amplified using reverse transcription polymerase chain reaction (RT PCR) with total RNA extracted from Ctenopharyngodon indellus kidney (CIK) cells treated with PolyI∶C and cloned into pMD20-T vector named pMD20-T-Mx, and sequenced. The recombinant plasmid pMD20-T-Mx and eukaryotic expression vector pEGFP-C1 were cleaved by restriction endonuclease, Mx gene full-length cDNA sequence linkaged with pEGFP-C1. The data indicate that grass carp Mx gene full length cDNA sequence has 1 921 nucleotides, encoding 627 amino acid residues of the mature peptide with a molecular weight of about 71.1 ku and a theoretical isoelectric point of 8.33. The grass carp Mx protein contains a triparitite guanosine 5′-Triphosphate(GTP) binding motif and a dynamin family signature sequence, which are conserved in all IFN induced Mx proteins of the vertebrates. Bioinformatics analysis reveals that the protein encoded by Mx gene in grass carp is hydrophilic in most regions and has rich B cell antigenicity positions without obvious nuclear localization signal sequence. The dimensional structure of the grass carp Mx protein includes GTPase domain in the amino terminus region, central interactive domain (CID) in the middle and GTPase effector domain (GED) in the carboxyl terminus region. Comparative analysis shows that the Mx protein amino acid sequence of grass carp shares similarity of 45.1%-99.7% to those of other species. pEGFP-C1-Mx, which contains 〗Mx gene full length cDNA sequence and EGFP gene, has been constructed.
LIU Jing , YANG Gui-mei , BAO Bao-long
2012, 21(4):509-515.
Abstract:Many unrelated bacteria in phylogenetic relationship with TTX producing ability were isolated from various animals and environment, reminding us that horizontal gene transfer (HGT) might exist among various TTX producing bacteria, but the exact mechanism is unclear. The objective of this study is to investigate whether HGT related genes exist in the plasmid pNe-1 isolated from tetrodotoxin producing Aeromonas sp. The tra, rum and vird4 genes were amplified by PCR. The 906 bp length tra fragment, 681 bp length rum fragment and 1 319 bp length vird4 fragment had significant homology to some transposase, relaxase and vird4 in sequence and contained conserved domains of these putative proteins respectively. The results indicate that the tetrodotoxin producing Aeromonas sp. has the ability to transfer genes by conjugation and transposition, and also has the ability to transfer TTX to the hosts. The result of research in this study is believed to be significant for the further understanding of the relationship among tetrodotoxin producing bacteria and the relationship between tetrodotoxin producing bacteria and the puffer fish.
LI San lei , XU Dong-dong , LOU Bao , XIN Jian , GENG Zhi , CHENG Guo-bao
2012, 21(4):516-523.
Abstract:The studied Japanese flounders (Paralichthys olivaceus) were divided into two categories: thermal tolerance stock and thermal susceptible stock by exposing in heated water (32 ℃). Amplified fragment length polymorphism (AFLP) and microsatellite technology were employed to screen the molecular markers correlated to thermal tolerance and analyze the genetic diversity of thermal tolerance stock and thermal susceptible stock of Japanese flounder. Eighty one AFLP primer combinations and forty two microsatellite primers were used to screen molecular markers correlated to thermal tolerance of Japanese flounder. AFLP and microsatellite loci which occurred in different genotype frequencies were analyzed using χ2 test to examine the correlation between the molecular markers and thermal tolerance in Japanese flounder. The results showed that the frequency of AFLP locus A3 was significantly higher in thermal susceptible stock than that in thermal tolerance stock, which indicated that this locus was negatively correlated with thermal tolerance, and the parameter of correlation was -0.453 (P< 0.01). Also, the frequencies of 4 AFLP loci were signicantly higher in thermal tolerance stock than those in thermal susceptible stock, which indicated that the 4 loci were correlated with thermal tolerance (P< 0.01). Based on microsatellite analysis, the loci S1 and S2 amplified by Po25A and 205TUF showed to be negatively correlated with thermal tolerance, and the parameters of correlations were -0.408 (P< 0.01) and -0.398 (P< 0.01), respectively. In addition, the genetic diversity of thermal tolerance stock and thermal susceptible stock were analyzed using eight AFLP primer combinations and six microsatellite primers. The results showed high genetic variations in the two stocks, and the genetic diversity of the two stocks was comparable. The results of present study could be helpful for the selective breeding of heat resistant flounders and provide reference basis for the stress resistance study of fish.
LI Bing , WANG Shuai , ZHANG Wei , Lv Wei-qun
2012, 21(4):524-529.
Abstract:Experiments were conducted to determine the feeding, growth and survival rate of eight month juvenile Pseudosciaena croceaduring low salinity domestication in an indoor circulating culture system. The juveniles were domesticated at salinity 10 ppt environment for 30 days and transported to the aquarium facilities at Shanghai Ocean University. After 8 hours transportation, the juveniles were cultured in indoor half closed circulating system at the salinity of 10. After 5 days environmental adaptation and 80 days of feeding period, the length of P.crocea increased from 5.31 cm to 8.36 cm, and the rate of growth was 0.04 cm per day. The weight increased from 2.59 g to 8.50 g, adding 0.07 g per day. The survival rate was 70.5%. Perhaps the commercial feed and the low grazing rate more or less lead to low growth rate of juveniles. Therefore, the result is expected to lay the foundation for the indoor desalination culture of P.crocea in the coastal reclamation and estuary district with low salinity waters.
LIU Min-hai , PENG Zhi-lan , ZHANG Feng-ping , LUO Hai-zhong , FU Rong-bing , LI Lai-guo
2012, 21(4):530-534.
Abstract:In this paper, We studied effect of the breeding density to the effect of Oplegnathus fasciatus in the growth,feeding and behaviour,at the water temperature of 23-24 ℃. The experiment was divided into four groups named D1, D2, D3 and D4, the initial desentiy was 1,2,3,4 kg/m3, respectively.The results showed that the larval Oplegnathus fasciatus mortality increased sharply beacause of the long term high density, which was disadvantageous to the lives of the larval Oplegnathus fasciatus. But at the low density, the larval Oplegnathus fasciatus growed very fast.While at the long term high density the growth was very slow, the specific growth rate dropped sharply(P<0.05).During the whole test, the feeding rate was decreased with the increase of the density in each tested group,the food net efficiency was higher in low denstiy than that in high density.In the whole test,the food net efficiency of D1 was the highest, then D2, D3, D4. We found that 6 kg/m3 was most suitable feeding denstiy of Oplegnathus fasciatus,while the density was higher than 9 kg/m3 which was unsuitable to the growth of 〖WTBX〗Oplegnathus fasciatus.
LI Xi-yang , BAI Jun-jie , FAN Jia-jia , YU Ling-yun , LIANG Xu-fang
2012, 21(4):535-541.
Abstract:To study the effects of morphological attributes on body weight of grass carp, 287 2 year old individuals were sampled for measuring seven metric traits, including the total length, standard length, head length, body width, body height, interorbital width, length before anus, The relationship between morphological attributes and body weight was quantified by correlation analysis, path analysis and multiple regression analysis to determine the major morphological attributes. The results showed that the correlation coefficients of each morphological trait to body weight were all very significant (P<0.01).Head length, body height were eliminated from the variable data, because of no significance in multiple regression analysis. Total length was also kicked out from variable data because it was co linear with standard length. The regression equation was obtained as Y=-3048.127+220.925X4+262.367X6+33.776X7+10.648X2, in which body weight(Y) was used as the dependent variables and body width(X4), interorbital width(X6), length before anus(X7), standard length(X2) were as independent variables. Judged from the results of high correlation index(R2 =0.900) it suggests that the main variables have been selected, among them the body width weighted the most(P=0.430)to the body weight, and it was key effective factor on body weight. In the selection process, four main morphological traits were considered as a whole,which provided essential theoretical basis and ideal measure for selective breeding of grass carp.
ZHANG Yu-ming , CHENG Shun , JIANG Jian-hu , LEI Shi-yong , YANG Liang-jie
2012, 21(4):542-548.
Abstract:Growth characteristics of generation 2010 ( 4 to 15 months after hatching) and generation 2009 (16 to 27 months after hatching) A. fasciatus were studied in Xinchang of Zhejiang province, including the male and female growth difference, growth feature and the change in body shape. The results show that there are quite significant differences on the total length (Lt), body height (Hb), body width (D) and body weight (W) between male and female, the growth rate of female is faster both generation 2010 and generation 2009 in this species. The growth equation of generation 2010 and generation 2009 A. fasciatus are Lt=0.268+4.932Hb(r=0.9568), Lt=0.236+9.014D (r=0.9941), W=8×10-3Lt3.087 (r=0.9921) andLt=1.255+4.726Hb(r=0.961), Lt=1.364+8.281D (r=0.9534), W=7×10-3Lt3.179 (r=0.9623), respectively. The generation 2010 A. fasciatus has a fast growth rate from April to August, whereas generation 2009 A. fasciatus grows slow from June to August in breeding period. During the whole growth process, there is difference on the body shape between male and female. There are quite significant differences on the total Length/body height, total length/body width, body height/body width, total length/head length, head height/body height and caudal peduncle length/caudal peduncle height indexes of generation 2010 and generation 2009 A. fasciatus each month, while the total length/head height index has no significant difference in the same case.
LV Yao-ping , CHEN Jie , BAO Bao-long , HUANG Pei-pei
2012, 21(4):549-553.
Abstract:To further understand the ossificational process of intermuscular bones in fishes from Cyprinidae, an important farming fish Hemibarbus labeo was investiaged in this study. Intermuscular bones of H. labeo at different developmental stages were stained with alizarin red. Before 35 dpf (days post fertilization), all bones in H. labeo were ossified except for intermusclar bones. Since 35 dpf, intermuscular bones in tail area begun ossification in 23.67 cm standard length H. labeo. The ossified epineurals were observed in the myoseptums from 37thto 41th myotome, and the ossified epipleurals was only found between 39th to 40th myotome. As H. labeo was developping, ossified intermuscular bones were gradually observed in the myoseptums close to the head. Till to 62 dpf, all intermuscular bones were found ossified in H. labeo with 30.03 cm standard length. In addition, the primary morphology of intermuscular bone was type I, and ossified intermuscular bones with more complicated morphology were gradually forming from the type I during ossifing process. On the whole, the timing of intermucular bone initial ossification and the forming process of complex morphology in H. labeo were very similar to that in other species in Cyprinidae, indicating conserved genetic mechanism control of ossification of intermuscular bones might exist in Cyprinidae.
CHEN De-hui , LIU Hong-sheng , HU Qing-song , WANG Wei-ding , ZHANG Shou-yu
2012, 21(4):554-560.
Abstract:Fish acoustic taming, as one of the technology in the control of fish behavior, have good promotion in fish control in sea ranching. Among the sound sources, habitat waters background sound of target fish based acoustic taming intends to involve better taming effect. To verify this, habitat waters’ background sound of Sparus macrocephalus is utilized to conduct the acoustic taming in a cage. The results based on the analysis of data and participant experiment observation showed excellent aggregation effect. Acoustic taming on juvenile Sparus macrocephalus needs three stages: acclimatization; impulsion of demanding food (outside sign is that fish reaches certain length and weight); building conditioned reflex between sound and bait. Sparus macrocephalus can identify different sounds and maintain longtime memory. As a strengthening method, offshore background sound based acoustic taming is a valid strategy to make fishes shift from short term memory to long term memory.
JI Lian-yuan , YANG Zhi-gang , QUE You-qing , GUO Zi-hao , ZENG Qi-tao , WANG Yao , CHENG Yong-xu
2012, 21(4):561-567.
Abstract:A feeding experiment which lasted 240 days was carried out to study the effects of different sizes and different diets on the edible part content and body composition of the female Chinese mitten crab (Eriocheir sinensis). The results showed that there was no significant influence on hepatopancreas index(HIS), gonad index(GSI) and meat percentage (P> 0.05) for different diets and size treatments. Chemical analyses of muscle nutrients showed that the contents of lipid, moisture and ash had no significant change for different sizes and diet treatments (P>0.05). As for muscle protein content of crab fed formula feed, the medium size was significantly higher than the small size (P<0.05), while for muscle protein content of crab fed trash fish, the small size was significantly higher than the large size (P<0.05) . The muscle protein content of small size crab fed trash fish was significantly higher than that fed feed (P<0.05). Chemical analyses of hepatopancreas nutrients showed that the contents of protein, moisture and ash had no significant change for different sizes and diet treatments (P>0.05). For hepatopancreas lipid content of crab fed formula feed, the small and medium size was significantly higher than large size (P<0.05), while for hepatopancreas lipid content of the crab fed trash fish, the small size and large size were significantly higher than medium size (P<0.05).The hepatopancreas lipid content of the same size crab, group fed trash fish was significantly higher than that fed feed (P<0.05). Chemical analyses of ovary nutrients showed that the content of protein had no significant change for different sizes and diet treatments (P>0.05). Moisture content of crab fed the formula feed had no significant change among the three sizes (P>0.05), lipid content increased significantly with breeding sizes (P<0.05), for ash content the large size was significantly higher than the medium and the small size (P<0.05). For lipid content of crab fed trash fish, the large size was significantly lower than the small size (〖WTBX〗P〖WTBZ〗<0.05). For moisture content of small size crab, group fed formula feed was significantly higher than that fed trash fish(P<0.05). For lipid content of the three size crab, group fed the trash fish was significantly higher than that fed formula feed (P<0.05). Combined, results in this study indicated that there was no significant influence on the edible part content of the female of Chinese mitten crab for different diet treatments and different breeding size treatments, while the body composition of the edible part had some differences because of breeding size or diet treatment(P> 0.05 or P<0.05).
WANG Rui-xue , WANG Di , LI Shao-wu , LU Tong-yan
2012, 21(4):568-574.
Abstract:Using high performance liquid chromatography with tandem mass spectrometric (HPLC-MS/MS), we established a new method for detecting florfenicol and florfenicol amine which enabled us to study florfenicol, related metabolites (florfenicol amine) pharmacokinetics and tissue distribution of Acipenser baeri after oral administration of florvenicol in vivo. In this study, oral administration of florfenicol was performed on the Acipenser baeri at a dosage of 15 mg/kg body weight at 22 ℃, and the blood plasma, liver, kidney and muscle were then sampled to measure the concentration of florfenicol and its metabolite (florfenicol amine) using HPLC MS/MS method. The results indicated that the concentration time course of florfenicol and florfenicol amine can be described by a two compartment open model with first order absorption in vivo. The maximal concentration(Cmax)of florfenicol in blood plasma was 3.4 μg/mL. Time point of maximal concentration(Tpeak)was 2.943 h. Volume of distribution(V/F)was 3.267 L/kg. Half life of elimination(t1/2β)was 31.21 h. Area under the time concentration curve(AUC) was 76.51 μg·h/mL, Cmax(FFA)/ Cmax(FF)and AUCFFA/AUCFF were 5.44%, 20.73%;Florfenicol and florfenicol amine were found to be extensively distributed and had a similar regularity in several tissues, with a higher concentration in liver and kidney. The results indicate that florfenicol have following features in Acipenser baeri: rapidly absorbed, high peak concentration, slow elimination and wide range of tissue distribution, and mainly metabolized or eliminated in the form of initial drugs.
JIA Zhi-ying , SHI Lian-yu , SUN Xiao-wen
2012, 21(4):575-580.
Abstract:Despite impressive progress in common carp farming during the last years, the impact of fish diseases is still an important problem in aquaculture. Current methods to control diseases consist, among others, of cultural environment control, medication or vaccination. Another alternative approach is to prevent diseases by improving the immune capacity of fish by or genetic selection. Selection breeding has made a great progress for common carp, but only limited to the growth characteristics, and resistance breeding research was only in the initial stage. In the future, common carp industry should focus on using of genetic variation, choosing suitable breeding methods, developing diseases resistance breeding program actively. In this review, stress effects on diseases resistance, genetic basis of diseases resistance, relationship between diseases resistance and other traits, evaluation and and related methods of breeding for the genetic improvement of disease resistance in common carp were given, at last, the paper give some information on the domestic latest progress in related fields.
LIU Chun-yan , XIE Man-jun , XU Shi-liang , LIANG Yi-xiang , LI Juan-ying
2012, 21(4):581-588.
Abstract:Tetracycline antibiotics removal by adsorption with activated sludge from wastewater was accomplished in this paper, and adsorption characteristics, adsorption kinetics, and adsorption equilibrium were investigated in a batch system. The effects of initial concentration, temperature and solution pH on adsorption were also discussed. The results indicated that adsorption equilibrium of TC, OTC and CTC can be reached at 120, 90 and 90 min respectively under the conditions that the initial concentration of antibiotics was 1-2 mg/L, pH was 6 and temperature was 25 ℃.The adsorption kinetics of all three antibiotics followed pseudo second order rather than pseudo first order, and the corresponding adsorption capacity qeq(mg/g) of TC, OTC and CTC were 1.638, 1.548 and 1.325, and the second adsorption rate constants k2 [×103g/(mg·min)] of TC, OTC and CTC were 65.52, 66.70 and 67.28 respectively. The Freundlich model fitted the experimental data better than the Langmuir model, and the isotherm equations of TC, OTC and CTC were qeq=8.485c1/0.915,qeq=8.170c1/1.063 and qeq=7.573c1/1.076 respectively. The optimal pH and temperature of all three kinds of antibiotics were 6.0 and 25℃. The higher the initial concentration was, the higher the adsorption capacity was, while it needed more time to reach equilibrium and the equilibrium concentration increased.
LIU Shou-hai , XU Zhao-li , TIAN Feng-ge
2012, 21(4):589-597.
Abstract:In order to study the diet composition and quantity of Coilia mystus, 1 355 stomach samples of C. mystus collected from Yangtze River Estuary and Hangzhou Bay from June to August in 2009 were analyzed in this paper. The feeding amount of Copepoda ranged from 0.10 ind/fish to 3.31 ind/fish in North Channel, Changxing Island, Shanghai, while 0.06-0.23 ind/fish in South Passage, Jiuduansha Wetland, Shanghai, which indicated that the feeding intensity of C. mystus in spawning ground was relatively higher than that during migration. It’s detected that the amount of Mysidacea was about 0.40 ind/fish in 100~150 mm size classes, and 0.10 ind/fish in 150-190 mm ones, which demonstrated that there was no obvious tendency of preying bigger Mysidacea by C. mystus in bigger size classes. Besides, the relationship between amount distribution of Copepoda and the size classes of C. mystus was not evident. Mysidacea was found both in stomachs of C. mystus and water samples collected from Hangzhou Bay, while there was a little amount of Mysidacea in C. mystus and none in water samples collected from Yangtze River Estuary. This showed that different composition of diet organism in various waters was the primary reason for the variety ofC. mystus feeding components. It’s also found that C. mystus showed a preference for Calanus sinicus (I = 0.89) and Acanthomysis longirostris (I = 0.86), while C. mystus had no active selectivity for dominant species in waters, such as Schmackeria poplesia (I = -0.39,Y = 0.30).
FANG Yong-qing , ZHONG Jun-sheng , MAO Cheng-ze , GE Cheng-gang , YANG Ping-hai , CHEN Yuan-ge
2012, 21(4):598-602.
Abstract:The surf zone of sandy beach in Tian’ao lies in a typical island harbor in the south of the Sijiao Island. In order to find out if there is any diurnal variation of species composition of larval and juvenile fishes, samplings were taken in surf zone of Tian’ao during the spring tide each month from October 2010 to September 2011 (seine net 1 m×4 m, 1 mm mesh aperture). A total of 33 species, 22 families, 484 individuals were collected which could be divided into 4 ecological patterns (marine fish 14; estuarine fish 11; migration fish 6; freshwater fish 2). The most abundant species Liza haematocheila was 29.5% of total individuals. The CPUE and species number of larval and juvenile fishes in the daytime were significantly higher than those at night(P<0.01). We also find out that CPUE of larval and juvenile fishes shows special distribution during different time periods in 24 h especially in the day time. There were more individuals collected during 6:00 8:00 and 14:00 18:00 than the rest of the 24 h. And the species numbers were higher during 6:00 8:00 and 12:00 18:00 than the rest of the 24 h. The results showed that larval and juvenile fishes prefer staying in shallow waters of surf zone in the daytime, and they had no obvious selection for surf zone in the night.
2012, 21(4):603-608.
Abstract:This paper introduces the effect of public participation in special marine reserves management from the connotation of public participation, and uses the public participation in Ma’an Archipelago special marine reserves management as the research object.From both resources protection and management strength the author analyzes the background of introducing the public participation in the reserves, then focuses on the enthusiasm and legal issues of the public participation and the problems of enforcing the public participation by introducing the methods of fishermen participating in reserves management.The article puts forward solutions to these problems: Enhancing consciousness of public participation; Improving the law of public participation in reserves; Giving the participants certain rights of management; Coordinating the problems of enforcing public participation in the management.
YAN Lei , ZHU Qing-cheng , ZHANG Yang , SHANG Li-lei
2012, 21(4):609-615.
Abstract:Based on the catch data collected by Chinese Pacific saury fishing vessels and remote sensing data in North Pacific, this paper analyzed the distribution of fishing ground of Pacific saury and their relationship with SST by using Explorer 4.0 on a grid of 1°×1°scale.The result indicated that the distribution of fishing ground changed widely in month, and it mainly concentrated in the waters near 36°N-47°N, 145°E-163°E; The movement of barycenter of catch had a tendency from south to north firstly and then moved in the opposite direction on the latitude, while on the longitude it moved from east to west. SST in the fishing grounds ranged from 10 to 17 ℃ with an average of 10-13 ℃.Meanwhile, SST decreased firstly and then increased as time goes on. Krnska1 Wallis test showed that there were no significant differences in CPUE related to temperatures during investigative period.
HAN Xiao-le , DAI Xiao-jie , ZHU Jiang-feng , TIAN Si-quan
2012, 21(4):616-620.
Abstract:Based on hook number information collected by scientific observers on the Chinese tuna longline vessels in the central Atlantic Ocean from 2007 to 2008 and from 2010 to 2011, the distribution of capture depth of swordfish (Xiphias gladius) was estimated by using catenary curve method. The depth range of the captured swordfish was from 124.6 to 280.5 m, with an average of 215.2 m and standard deviation of 46.5 m. With the increase of depths of hooks ,the proportion of swordfish in total catch increased to a maximum at the depth of 220 m to 250 m and then decreased. The proportion of female swordfish in catch increased with depths of hooks and then remained stable. The mean fork length of swordfish tended to increase with depths of hooks. This paper provided important information for improving our understanding of swordfish habitats and bycatch mitigation in the pelagic tuna fisheries.
HU Song , CHEN Chang-sheng , GAO Guo-ping , LAI Zhi-gang , GE Jian-zhong , LIN Hui-chan , QI Jian-hua
2012, 21(4):621-629.
Abstract:This study is aimed at validating the Global Finite Volume Community Ocean Model (Global FVCOM) for tidal simulation in the East China Sea (ECS) (24°N to 41°N and 118°E to 132° E). The model data comparisons were made for the amplitude and phase lag of M2, S2, K1 and O1 at 99 observations along the coast of the ECS. Generally, the model results agreed well with observations, except for areas where the resolution of grid does not resolve the local bathymetry well. The amplitude and phase lag root mean square errors are 15 cm and 10° for M2 tide and 8.2 cm and 9° for K1 tides. The results of Global FVCOM provide a foundation for further development on establishing a multi scale global regional ocean model for the ECS research.
2012, 21(4):630-634.
Abstract:Chinese colleges and universities have reached a new stage of development, at which they are shifting from scale expansion to internal quality improvement. So they are clear about their own developing orientation after making their long term planning. The subordinate colleges are the subsidiary units under the universities and directly responsible for achieving the universities’ developing goals; perfecting the management system of subordinate colleges has become the direction of reform of Chinese universities’ administrative management system. The joint conference system between the Party and the administration is the correct choice of the subordinate collage management system along with the tide of historical development; the joint conference system is an organic part of the modern university system with Chinese characteristics, and is the scientific practice to improve the modern university system; the joint conference system can promote democratic management,and is good for the Party to play its role, and is an effective measure to improve the scientific level of the party construction; the joint conference system built the operational mechanism of joint responsibility of Party and administration, and is needed to adapt to situations with Chinese characteristics, and is the inevitable choice of colleges and universities in China. The exploration of the theory and practice of the joint conference system between the Party and the administration of subordinate colleges can provide reference for the deepening of reform of university management system.
2012, 21(4):635-640.
Abstract:The culture protection and development have become hot topics all over the world, and the economic functions of the culture causes the universal attention from all sectors of society. As a new industry, cultural industry has characteristics of high technology, high intelligence, high efficiency, low consumption and low pollution, and it is also known as the “rising sun industry” in today’s society. Marine culture industry is an important part of the culture industry which has very good development prospect and potential value. The industrialization of culture industry is an important way that cultural resources achieve its own economic value under the conditions of market economy. It can both drive economic development, and develop the traditional culture, with a strong practicality and reality. Shanghai has rich marine culture resources, with a long history and a wide range of category and it almost includes all types of marine culture in China. At the same time, Shanghai also has many marine culture resources with unique features, such as Shanghai’s seawall culture, sea salt culture, fishing village folk beliefs and so on. Through the investigation, arrangement and analysis of these resources, we can reasonably exploit and protect these resources. In the case of fully realizing the economic value of marine culture resources, we should avoid the loss of resources and make sure the consistency and sustainability of the cultural resources. For governments, they should play the leading role, set up the property rights consciousness, support the development of relevant industries and create the brand.
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