Abstract:Zebrafish oocyte maturation was induced by various concentrations of FSH and 17α,20β-DHP. The results indicated that FSH of 1IU/mL and 17α, 20β-DHP of 5ng/mL could initiate significantly germinal vesicle breakdown (GVBD) of zebrafish, separately, while the 17α, 20β-DHP of 5ng/mL had better performance. The analysis of SDS-PDGE and Western blot showed that 17α, 20β-DHP of 5ng/mL activated oocyte MAPK and increased GVBD in the zebrafish. The histochemistry assay showed MAPK was activated at the stages Ⅱ and Ⅲ of oocyte development in the plasm while no MAPK activity was observed at the stage Ⅰ. At the stage Ⅴ, the activated MAPK could be observed both in the plasm and nucleus while GVBD took place. The results indicated that activated MAPK played an important role in promoting the ovary development and began to move from the plasm to the nucleus while it was activated.

Abstract:The effects of different dissolved oxygen (DO) concentrations, including severe hypoxia(0.2, 1.0, 2.0 mg/L), hypoxia(3.0, 5.0 mg/L), normoxia(normal aeration as control, about 7.0 mg/L) and hyperoxia(11.0, 14.0 mg/L), on nucleus anomaly in erythrocytes, superoxide dismutase(SOD), catalase(CAT) activities in serum and malondialdehyde(MDA) concentration in liver were studied in juvenile turbot Scophthalmus maximus. Fish mortality was only observed in the 0.2, 1.0 and 2.0 mg/L groups, in which all fish died in 2 h, 24 h and 48 h, respectively. The effects of DO on nucleus anomalies were shown with obvious timecumulative effects and dosecumulative effects in the 28day experiment. The micronuclei were first observed in the 5.0, 14.0 mg/L and control groups on day 28. They were still found in hyperoxia and control groups after recovery for 14 days. The SOD activities in different groups showed significant difference at each sampling time (P<0.05). The difference in CAT activities among experiment groups was significant only after 7day exposure (P<0.05). The MDA concentration increased with the increasing concentration at 2 h. The MDA concentration in hypoxia and hyperoxia groups decreased with time duration. After 14day recovery, the MDA concentration in hypoxia groups was significantly higher compared with those in control groups (P<0.05). Additionally, the oxidative stresses were also found in control groups. No obvious changes on the total nucleus anomalies and other antioxidative index were found in 5.0 mg/L groups.

Abstract:The aim of the present study was to determine the effect of starvation on body appearance,muscle Composition and blood physiolgical of the carp (Cyprinus carpio) at the water temperature 18-20℃ in the cycle and flow water aquarium. The experiment was designed for four groups with three replication, which were 0 day(control), 10 days, 20 days, 30 days group. The results showed：With the carp hunger time extending,there was a trend to decline for the obese relative,intestine and body length ratio, live index of the carp, but the effects was not significantly(P>0.05); there was a trend to increase for contents of moisture, crude ash in the body muscle of the carp,but a trend to decline for the crude fat in the body muscle of the carp, and the differences was not significantly (P>0.05),while body contents of crude protein was remarkable difference（P<0.05）; With the carp hunger time extending, red blood cell quantity and hemoglobin value dropped significantly（P<0.05）,and white blood cell quantity decreased not significantly (P>0.05), blood glucose levels rasied first and then went down. The experiment illustrated that the carp body appearance had changed, and with the prolongatin of starvation,the muscle fat was decompose to exploit first,the muscle protein then to be used,at the same time ,the body physiological of the carpwas changed to be fit for the starvation condition.

Abstract:The morphous and ultrastructural organization of Salvelinus leucomaenis spermatozoon was observed by scanning and transmission electron microscopes. The spermatozoon with no acrosome consisted of the head, middle piece and the tail. The head of the spermatozoon was oval shape and mainly consisted of the nucleus. The nucleus, within which there were some netlike gaps, consisted of electron dense chromatin materials. In posterior extremity of the nucleus, implantation fossa trapped into the internal to the 1/3. The middle piece consisted of centriolar complex and sleeve. The proximal centriole, which is made up of nine triplets of peripheral microtubules, is perpendicular to the distal centriol which extended downward to form the kinetosome of axial filament. The sleeve, which contained a high number of mitochondria and two kinds of vesicles, was connected to the posterior end of the nucleus. Some mitochondria were fused with each other and formed a complex chondriosome. The tail of 〖WTBX〗Salvelinus leucomaenis〖WTBZ〗 spermatozoon was slim and long. The main composition of the tail was axoneme whose structure was the typical model of “9+2” and surrounded by vesicles. The plasma membrane was prominent symmetrically up and down to form two lateral fins with different sizes.

Abstract:In biological evolution research area, the embryo and larva of sea anemone are important experimental animal to find the bilaterian evolutionary developmental ancestor. Based on the early developmental process and morphological characters of the sea anemone, Cereus sinensis, in the laboratory, it’s embryo development, planula stage, larval settlement and juvenile polyp developmental stages were described in this study. At the each stage, the changes of morphological characters, shape size and inner structure of zygote, egg cleavage, planula larval and polyp was observed along with the early developmental sequence. Photo picture was recorded in the study to present the live status. The difference of larval settlement between sea anemone Cereus sinensis and jellyfish Rhopilema esculentum was discussed. The comparison on early development of Cereus sinensis with three other species of sea anemones was made to elucidate the distinction of tentacle eruption. The process of metamorphosis from the oocyte to the 16 tentacles juvenile polyp of the sea anemone Cereus sinensis was illustrated.

Abstract:The aim of the present study was to investigate the effects of different temperatures and salinities on the survival and development of larva Trachinotus ovatus. The newly hatched larva was transferred from sea water (24 ℃, salinity 32) to and kept in a combination of four temperatures (18 ℃, 21 ℃, 24 ℃, 27 ℃) and six salinities (16, 20, 24, 28, 32, 36). After 1 day, the survival of larva Trachinotus ovatus exposed to combinations of salinities 16, 20, 24 and 21 ℃, 24 ℃, 27 ℃ are the lowest, less than 10%. The mortality of larva at salinities 28, 32, 36 were lower, less than 50%. Surprisingly, the survival rates of larvae at 18 ℃ with different salinities were higher, more than 50%. Our experiment showed that temperature plays a critical role in larva development.The larvae developed faster at higher temperature to a certain extent, the development of larvae Trachinotus ovatus became slow or even stopped at 18 ℃. Taking both survival and the development conditions into account, we draw a conclusion that the preferable temperature and salinity for larval survival and development is 24 ℃and 32, respectively.

Abstract:The primary observation on propagation and study on artificial reproduction of Barbatia virescens were performed from 2009 to 2010，which revealed the processes of propagation and embryonic development. The feasible environmental conditions for hatching, larval growth and settlement of young shellfish were selected via experiments. The natural breeding season of Barbatia virescens in the south of Zhejiang Province is from June to July，when water temperature is 24-28 ℃. The ranges of suitable temperature and salinity in hatching were 24-28 ℃ and 22-26 respectively, and the initial Dstage veliger appeared about 18 h after fertilization. In Dstage larvae，the feasible temperature range was 24-28 ℃，feasible salinity was 26 in larval growth, and the dainty food was Isochrysis galbana. After about 10-12 days rearing, the larvae develop to the stage of metamorphosis. The hard cement brick with rough surface was suitable for attaching and growth.

Abstract:An 8week laboratory trial was designed to study the effect of phytase on growth, body composition and phosphorus content in tissues of juvenile grass carp (〖WTBX〗C. idellus〖WTBZ〗) fed plant protein based diet. Seven trial diets were formulated to feed fish (with mean initial body weight of 39.68±3.05 g) that were divided into 5 experimental groups and 2 control groups. Five triplicate experimental groups were supplemented with five different levels of phytase (500 U/kg, 750 U/kg, 1 000 U/kg, 1 250 U/kg, 2 500 U/kg). The positive control group was supplemented with 1.5% monobasic calcium phosphate; and the negative control group was not supplemented with phytase and monobasic calcium phosphate. Through 60 d experiment,the effects of phytase were examind on growth, body composition and phosphorus content in tissues of juvenile grass carp(C. idellus)fed plant protein based diet.The results showed that，the feed conversion rate (FCR) was significantly lower, whereas protein efficiency ratio (PER) and specific growth rate (SGR) were significantly higher than those of other groups (P<0.05), when only monobasic calcium phosphate was supplemented. Compared with negative control group，after added phytase in diet, when the supplemented level of phytase was higher than 1 000 U/kg, the FCR of experimental group 3 (1 000 U/kg) was significantly lower than that of negative control group (P<0.05)，and PER was significantly higher than that of negative control group (P<0.05); The phytase has no effcets on the HSI, VSI, and CF of fish; the content of crude fat decreased in body, when the phytase content was higher than 1000U/kg, when the supplemented level of phytase content was 1 000 U/kg, the crude fat in muscle was significantly improved; The phosphorus content was enhanced to some degree in body, muscle and spine after added phytase, and when the content was 1 250 U/kg, the phosphorus content were significantly improved in muscle and spine. The result indicated that, when the supplemented level of phytase was 1 000 U/kg-1 250 U/kg, the SGR, PER and phosphorus content of grass carp were enhanced, and FCR was improved compared with negative control group. If you want to reduce the supplemented level of monobasic calcium phosphate, the suitable supplemented level of phytase was 1 000 U/kg-1 250 U/kg.

Abstract:The present experiments were conducted to investigate the moisture content, lipid class and fatty acid composition in mature ovary and early embryo of Portunus pelagicus. The results indicated that: (1) Although the early embryo of P. pelagicus had higher moisture content than that of mature ovary, the higher total lipid/wet tissue could be found in the mature ovary than that of early embryo; (2) There were no significatn differences in the triacyglycerol (TG) and phospholipids (PL) contents between mature ovary and early embryo, however, the early embryo had higher free fatty acids and cholesterol contents than the mature ovary. This could be explained by the hydrolyzation of TG and PL; (3) There were twenty seven identified fatty acids that could be detected in the both mature ovary and early embryo. The significant differences existed only in 15∶〗0, 14∶1n7, 20∶1n7 and 18∶3n3 between mature ovary and early embryo while the main fatty acids were 16∶0, 18∶0, 16∶1n7, 18∶1n9, 20∶5n3 and 22∶6n3 for both tissues.

Abstract:Using reversed phase high performance liquid chromatography(RPHPLC), the pharmacokinetic differences of difloxacin(DIF) with a single dose of 20 mg/kg body weight after oral administration were compared in crucian carp （Carassais auratus gibebio） at the water temperatures of 16 ℃and 25 ℃.Analyzed with Practical Pharmacokinetics Program (3P97)，the results show that the kinetic profiles of DIF，which have significant differences within the two grades temperature, conformed to a twocompartment model, and the absorption halftimes(T_1/2Ka) were 0.009 9 h and 0.122 2 h at 16 ℃and 25 ℃, which were found to have a positive relationship with water temperature, while the elimination halftimes（T_1/2Ka: 70.968 and 18.322 h）,the apparent distribution volumes（Vd/F: 1.875/kg and 0.676/kg）,the area under the concentrationtime curves ［AUC,μg/(mL·h)］ of plasma (763.761 and 243.244) and tissues (746.622，1 095.711，1 222.75and 294.857，258.587，344.63 in liver, kidney and muscle ,respectively) have negative relationships with water temperature. Besides the metabolite of DIF, sarafloxacin (SAR), all can be detected in the plasma and tissues(liver, kidney and muscle) at 16 ℃and 25 ℃, whose kinetic profiles have multimodal phenomenon. The results indicate that the distribution of DIF at 16 ℃ is wider than that at 25 ℃. In addition, the time, when ratio of C_max/MIC₉₀≥10 was greater than or equal to 10 at 16 ℃,could reach 72 h，while it was 4-6 h at 25 ℃;Therefore, DIF may have higher efficacy at low water temperature(16 ℃).

Abstract:In order to breed Rhodospirillaceae bacteria with high temperatureresistance and saltresistance for powdery agentmanufacture, the conditions of protoplast fusion between saltresistant Rhodospirillaceae bacteria which could degrade Nnitroso over 90% and Bacillus stearothermophilus which grew at 60-70 ℃ were studied using orthogonal test. The physiological characteristics of fusants such as temperature, growth salt and purifying the water were determined and the identifications between parent strains and the excellent fusant by electron microscope and absorption spectroscopy were studied. The results showed that the optimal conditions of protoplast fusion were as follows: temperature 37 ℃, reaction time 3h and the adding amount of PEG 40%.Under these conditions, the rate of protoplast fusion was 1.536% and three fusants(a,b,c) which could grow well at 30-50 ℃ were obtained. The tolerance of growth temperature of three fusants was improved by 66% than saltresistance Rhodospirillaceae bacteria, but some characteristics of saltresistance ,degrading N ammonia and Nnitroso were similar to it. Fusant a grew faster and steadier than other fusants and its characteristics of cell shape, Gram stain and pigment absorbing peak were similar to saltresistantRhodospirillaceae bacteria.

Abstract:The fish stock assessment is important groundwork for presentday fish stock management.The report presents a stock assessment of small yellow croaker in East China Sea by Bayesbased PellaTomlinson model,and a forecasting of biomass and catch from different fisheries policy in 2003-2020. All of the data used in this study are from the monitoring for fisheries resources in East China Sea.The research results show that the carrying capacity K is 410 281tons and the intrinsic growth rate r is 1.129;the estimated stock biomass increased from approximately 332 654 tons in 1991 to 370 923 tons in 1994 and then declined to the minimum 131 416 tons in 2003;the estimated MSY is 110 000tons; the estimated biomass for achieving MSY is about 200 000 tons ;the estimated effort for achieving MSY is 1 373 380 nets; the stock depletion level (Bcur/K ) is about 0.319, i.e. the current biomass is less than by 40 per cent of the initial biomass;if using less than 0.4 of fishing mortality after 2004, this will bring about an optimal utilization to the fishery.

Abstract:Resources multiplication was evaluated preliminarily based on 3 surveys carried out in March of 2004, May of 2007 and August of 2010 respectively. The results show that: after the development of artificial reef (ARs) in reef area, its species number was 1.50 times in 2007 and 2.15 times in 2010 of the number in background survey. The species numbers in control area were 1.27 times in 2007 and 2.00 times in 2010 of the number in background survey. Resources density in ARs area became 2.13 times in 2007 and 3.00 times in 2010 as many as in 2004. Resources densities in control area were 0.46 times in 2007 and 1.96 times in 2010 of density in 2004. Pileou uniformity index, ShannonWinener diversity index and Margalef species richness index increased gradually after ARs were deployed in both ARs area and control area. Fish has become the dominant species in both ARs area and control area after ARs were deployed. Some kinds of economic species which did not appear in background survey became normal species in tracking surveys. It is concluded from those results that the fishery productively in ARs area has improved a lot, and the community structure has became multiplication and stabilization. The resources multiplication effect and economic effect of ARs in Wushi Leizhou have been proved to be efficacious.

Abstract:Based on the samples from Chinese squid jigging vessels in the traditional fishing area (150°-165°E，39°-45°N) of northwest Pacific in 2007, the biological characteristics of squid Ommestrephes bartramii were studied. The results showed that the range of mantle length was from 200 to 436 mm and the average mantle length attained 282 mm, the dominant mantle length ranged from 230 to 320 mm which accounted for 81.6 percent of the total. The monthly mantle length of male squid was smaller than that of female, the growth index of relationship between mantle length and body weight was nearly three. The sex ratio of total number accorded with 1∶1（P>0.05）, but the proportion of mature males was obviously higher than that of females（P<0.05）. The sexual maturity stage dominated Ⅰ and Ⅱ, the average maturity stage of females was less than that of males. The length and weight of nidamental gland of squid increased with the growth of gland maturity. Since the composition of mantle length had two modes in samples and immature and mature individuals both exist, it is concluded that the individuals of the catch could be divided into two populations in the northwest Pacific Ocean, i.e. winterspring cohort with smaller size and autumnspawning cohort with bigger size, in which the winterspring cohort dominated in the catch.

Abstract:A total of 211 Chilean jack mackerel (Trachurus murphyi) were collected by a largescale trawler named “KAIXIN” from July to October 2010 in the southeast Pacific Ocean. The morphologic features of their sagittas were observed and measured. The sagittas of Chilean jack mackerel have four parts: front part, back part, dorsal part and ventral part. It has an apparent rostrum which is longer than the antirostrum. The result of principal component analysis on 24 morphologic indices shows that the total sagitta length (L₁), distance from the core to the end(L₃), distance from the end to the antirostrum(L₁₁) and distance from the end to the intersection(L₁₃) can reflect the length features of sagitta, and distance from the core to the ventral(L5), distance from the end to the ventral(L₁₀), distance from the ventral to the intersection(L₁₈) and antirostrum length(L₂₃) can reflect its width features. The relationships between the featuredlength of sagitta including L₁, L₃, L₁₁ and L₁₃, and fork length as well as body weight were best described by power functions(P=0 <0.05). Also, the relationships between the featuredwidth of sagitta including L₅, L₁₀ and L₁₈, and fork length as well as body weight were best described by power functions(P=0<0.05). Antirostrum length (L₂₃) was best described by exponential functions with fork length as well as body weight (P=0<0.05).

Abstract:Geographic information system (GIS), as a powerful spatiotemporal analysis tool, has been applied to marine fisheries since the late 1980s, and which is widely used in the fields of data processing and analysis of fishery conditions, relationship between fishery resources and environment, aquaculture selection, fishery resources assessment, tagging, marine ecosystem and fishing ground forecasting. But GIS in the marine fisheries still faces with great challenges. The information of fishery resources includes not only fishery data and environmental data, but also socialeconomic data which are closely related with fishermen. That is, in the future GIS in marine fishery should be a set of natural resources and environment systems, human systems, social systems and economic systems. Fisheries management and sustainability based marine ecosystem has been a common concern of community and international organizations, and GIS has greater advantages than traditional methods and will further enhances fishery decisionmaking. The applications of GIS in fisheries will focus on: （1） the establishment of the database and data standardization; （2） the design of key essential fish habitat; （3） the definition of marine protected areas; （4） longterm monitoring and management of resources; （5） distribution and migratory of fish specis and 3D analysis of marine environment. Finally, the establishment of GISbased framework for ocean fisheries information system in our country is proposed.

Abstract:The Chinese basic legal system of environmental protection and management in fishery waters was preliminarily formed in 1980s, which has been adjusted and strengthened since 1990s. On the basis of the integrated waters environmental protection and management system, the Chinese basic legal system of environmental protection and management in fishery waters preliminarily formed fishery waters environmental monitoring and evaluation system, fishery waters quality standard system, systems on inquiry, settlement and compensation on fishery pollution accidents, specialized system on injury prevention, remedy and compensation of ecological environment in fishery waters. The construction of legal system has gained achievement, but there are still some problems: （1）Matching regulations on relevant basic legal rules of Marine Environmental Protection Law, Water Pollution Prevention Law and Fisheries Law are imperfect, which leads to the legal system poor enforceability. （2） Part of critical laws are undefined, which has some difficulties on enforcement or is unable to enforce. （3） The current legal system failed to enforce strictly. （4）Some of the critical legal systems are imperfect. Suggestions: Make integrated special legislation on fishery waters environmental protection and management. Modify or reformulate rules and regulations for implementation on Fisheries Law and Water Pollution Prevention Law. Implement current legal system strictly. Take an active part in developing the system and mechanism on environmental protection and monitoring of fishery waters.

Abstract:In order to find out the impact of dumping dredged materials on Lanshan Port Temporary Ocean Dumping Site areas, with the methods of single factor index and eutrophication index,we evaluated the monitoring data of the seawater and sediments quality over years, and analysed the changes of marine biology and the water depth. The results show that the water quality of the investigated areas reached the secondclass water quality from The Chinese National Standard of Seawater Quality. In addition to inorganic nitrogen, other quality indices of the evaluation factors are generally more stable, fluctuate within a certain range. The sediment quality of the investigated areas reached the firstclass sediment quality from The Chinese National Standard of Sediment Quality, and the sediment quality indices of the evaluation factors fluctuate within a small range. Dumping dredged materials caused a temporary reduction in the total density and the total number of phytoplankton species. At the same time dumping dredged materials caused an obvious raised topography in the north and east area of the Lanshan Port Temporary Ocean Dumping Site. The results show that except the water depth had been impacted, the dumping of dredged materials has not caused significant impact on the ecological environmental quality of Lanshan Port Temporary Ocean Dumping Site areas.

Abstract:The contents of heavy metal Cd, Cu, Pb, As, Hg and Zn in shellfish were analyzed, and the space distributions and seasonal variation features of these heavy metals in oyster were discussed, according to the survey results of oysters, clams and pinnidaes from the coast of South China Sea in 2009. The statistics results showed that the contents of Cd, Cu, Pb, Hg, As and Zn in shellfish had obvious differences among various sampling areas, species and cultivation seasons. The Cu and Zn contents in oyster samples were much higher than those in clam and pinnidae samples. The diet exposure quantity of heavy metal in shellfish samples were calculated applying the crude point assessment method. The assessment results showed that, for a healthy adult, the exposure quantity of heavy metal by intake of oysters and clams was lower than the recommended values of JECFA. While the Cd exposure level by pinnidaes intake was higher than the recommended values of JECFA.

Abstract:In order to evaluate the improvement of water quality by the construction of the ecofloating bed and the bank restoration project in the Yinshui River of the Dripping Lake, indicators like nutrient ,transparency, chlorophyll, as well as phytoplankton density and distribution have been investigated in this study, which was carried out druing the Spring 2010. In addition, the biological diversity index of phytoplankton was calculated and TLI method was used to estimate the sites eutrophication level. Results: (1) the physical and chemical indicators variation trend demonstrated that the project had improved the water quality to some extent, with the TN removal rate 47.9% and TP removal rate 46.8%; (2) the variation of phytoplankton density showed a significant positive correlation with temperature and a significant negative correlation with TN/TP, and eutrophication was found in all sites through density evaluation. (3) the sites TLI ranged from 54.58 to 72.75, with the average of 64.07, which indicated a mesoeutrophication level with severe eutrophication in Site 1.(4) the comparison of ShannonWiener Index(H’) and Pielou Evenness Index(J) showed that most of the samplings were in the states of moderate and severe pollution, with the similarity of 52.4%.Besides, ShannonWiener Index(H’) demonstrated the pollution degree of the site in the rear of riparian buffer reduced. Finally, the number of potential pollution sources around the Dripping Lake is large while the water changing period is long and the community of phytoplankton is different from other freshwater lakes because of its special beach sediment. Considering the newlybuilt water ecological system of the Dripping Lake and its water sourcing rivers is not stable yet, longtime monitoring and assessment are of significant value especially for manmade lakes.

Abstract:To satisfy the requirement of density of aquatic for shrimp and crab pond aquaculture, the aquatic should be cut periodically. Based on the analysis of traditional manual reaping and river cleaning equipment, a whole new plan for reaping aquatic in shrimp and crab pond and designs of aquaticcuttingharvesting equipment have been proposed. Furthermore,demands were made on the size and data of exact dimensions and other important parameters of main working parts, which were based on the actual production needs. Hub cutter is analyzed by CFD software FLUENT in order to get the integrative data about in the field of dynamic pressure. The equipment is set to use cutter and worm conveyer with easy geometry, dehydrating in real time, without pumps, with good stability, high efficiency and adapting to any kinds of difficult working environments. Such equipment satisfies the needs of low cost,easy for assembling proposed by the actual demands.

Abstract:The standard system of fishery energysaving and emissionreduction is established for the standardization of energysaving and emissionreduction in fishery. This standard system also serves for the achievement of the ordering of technology and management. The standard is based on the energysaving and emissionreduction technology, and its standardization has a strong supporting on the innovation and extension of energysaving and emissionreduction technology. The aim of the research on the standard system of fishery energysaving and emissionreduction is to establish the standard system according to the Directives for the Work of Standardization of the People’s Republic of China, that is,“Principles and Requirements for Preparing Diagrams of Standard System”, and “Guideline for the Work of Complex Standardization”, in order to direct the revision of Chinese fishery energysaving and emissionreduction standard, to promote the combination of technology innovation and extension, and to meet the needs for sustainable development of Chinese fisheries. This paper briefly described the current status of fishery energysaving and emissionreduction standard, and listed the relevant standards needed to enact in the near future through the research on the standard system，and constituted fishery energysaving and emissionreduction standard system.

Abstract:A widespread social concern has been caused by the corruption with high frequency in universities in recent years. Only by providing against possible trouble,can incorruptibility risks be controlled and reduced. Both motivation and opportunity are the central factors leading up to corruption. Opportunity is the external cause. It comes into existence because of the loss of restriction system for the authority. Motivation is the internal cause. It can be translated into action because of the collapse of ideological and moral defence lines. Earlywarning to the power operation not only relies on the system building and supervision with technological means that can reduce corruption opportunity, but also relies on the culture infiltration that can firmly construct ideological and moral defence. It’s the key element in preventing incorruptibility risks efficiently to strengthen the system construction, raise the incorruptible consciousness and create the easy supervision environment. The earlywarning and prevention mechanism of incorruptibility risks based on system, culture and technology, that can combine binding force of system, persuasive power of the education and balance power of supervision, gives us satisfying choice. Only by ramming the cornerstones of system building，supervision with technological means and culture penetration，can we achieve the goal of preventing and controlling corruption from fountainhead from our study.

Abstract:To improve teaching forms and methods and to improve consistently the pertinence and effectiveness of ideological and political theory course in university are the basic tasks of new round teaching reform of ideological and political theory course. But the large classes that existed widely in China's universities after its mass expansion have become the bottleneck of taskperforming. For solving this problem, it is insufficient to optimize the large class teaching, and it is necessary to reduce the size of large class. Under current conditions, the medium class teaching is a feasible alternative. In this paper, a comparative study on large class and medium class of ideological and political theory course in university has been made. By analyzing the respective characteristics of large class and medium class, and revealing the different effects of these two teaching forms to the teaching effect, the necessity of medium class teaching is presented,and some basic problems about how to implement medium class teaching are investigated. From this study we have drawn the following conclusion: by implementing medium class teaching, the pertinence and effectiveness of ideological and political theory course in university can be greatly improved, and to change the class size from large class teaching to medium class teaching is concerned with not only the number of students, but also corresponding changes of the teaching forms and methods, teaching resources, teacher management system, and so on.