- Volume 0,Issue 2,2010 Table of Contents
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SHEN Jin-yu , LI Xin-hua , PAN Xiao-yi , YIN Wen-lin , CAO Zheng(Zhejiang Institute of Freshwater Fisheries , Huzhou , China)
2010(2).
Abstract:Aeromonas sobria TL97528,isolated from soft-shelled turtle(Trionyx sinensis),is the pathogen of Trionyx sinensis bacterial disease.And serine protease is the major virulence factor of aeromonas.A pair of primers was designed according to the nucleotide sequences of the serine protease gene of aeromonas species reported in GenBank.Desired fragment about 809 bp was amplified from TL97528 genome by the specific primers.The PCR product was cloned into PMD 18-T vector and sequenced.The sequence has been deposite...
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SHEN Li-hong , XIONG Liang-wei , TANG Yong-kai , WANG Shuai-bing(.Shanghai Pudong New Area Aquatic Product Technical Advice Station , Shanghai , China , .Jiangsu Animal Husb , ry , Veterinary College , Taizhou , .Freshwater Fisheries Research Center , Chinese Academy of Fishery Sciences , Wuxi , China)
2010(2).
Abstract:通过PCR法从奥利亚罗非鱼DNA中扩增出MSTN基因。该序列全长1 957 bp,含有3个外显子,2个内含子。外显子Ⅰ379 bp,外显子Ⅱ371 bp,部分外显子Ⅲ145 bp,内含子Ⅰ305 bp,内含子Ⅱ751 bp,编码区895 bp,共翻译298个氨基酸。通过随机测序法筛选SNPs,获得了6个突变位点,外显子Ⅰ、Ⅱ各有一个,而内含子Ⅱ则含有4个突变位点。外显子Ⅰ、Ⅱ的突变位点均导致翻译的氨基酸序列发生改变,为错义突变。研究结果为SNPs位点与奥利亚罗非鱼生长性能关联分析奠定了基础。
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YANG Wu , ZHAO Jin-liang , DENG Yan-fei , CHU Jun , HUANG Ai-ping(.Key laboratory of Aquatic Genetic Resources , Utilization , Ministry of Agriculture , Shanghai Ocean University , Shanghai , China.Tianlu Precious Aquatic Products Co.Ltd , Taizhou , China)
2010(2).
Abstract:Siniperca scherzeri steindachner is one kind of freshwater species which is exploited and widely cultured recently,however,propagation at large scale is still on the way.To further understand the general characteristics of ovary maturation development,ovary development stages and the content of serum phosphoprotein(SPP) of S.scherzeri collected from Changjiang River from mid-March to mid-May were analyzed by histological and biochemical metheds,respectively.During this periods,primary oocytes of S.scherzeri...
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WANG Wen-juan , CAI Chun-fang , YE Yuan-tu , SHI Chen-jiang , XIA Yan-mei(College of Basic Medical Science , Biology Science , Soochow University , Suzhou , China)
2010(2).
Abstract:从异育银鲫外周血中分离淋巴细胞,利用RPM I-1640培养基培养48 h后,采用流式细胞仪分析方法研究不同浓度的植物凝集素(phytohem agglutin in,PHA)(0μg/mL、80μg/mL、200μg/mL、400μg/mL、600μg/mL、800μg/mL、1 000μg/mL、1 200μg/mL)对外周血淋巴细胞转化率的影响,旨在探索异育银鲫外周血淋巴细胞体外转化最适刺激剂量。结果表明:(1)在PHA浓度0~400μg/mL的范围内,随着PHA浓度的增加,淋巴细胞转化率也增加,呈浓度依赖的关系,但当PHA的浓度增加到1 200μg/mL,淋巴细胞转化率明显下降。其中PHA浓度为400μg/mL时,淋巴细胞转化率最高,显著高于低浓度组(80μg/mL)和高浓度组(1000μg/mL、1200μg/mL)(P<0.05),略高于中浓度组(200μg/mL、600μg/mL、800μg/mL),差异不显著(P>0.05)。由此可见,在本试验条件下,PHA体外刺激异育银鲫淋巴细胞转化的最适剂量为400μg/mL。(2)用流式细胞仪检测细胞内DNA合成情况,Mu lticyc le AV软件进...
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PENG Zhi-lan , LIU Min-hai , FU Rong-bin , LUO Hai-zhong , XIA Feng-feng , XU Zhi-jin(.Fisheries Institute of Zhoushan , Zhoushan , China , .Zhoushan Marine Survey , Design Institute , .Zhoushan Fishery Technical Extension Station , China)
2010(2).
Abstract:Genetic diversity of a wild population and an artificial breeding population of Miichthys miiuy was investigated with amplified fragment length polymorphism(AFLP).The wild population was caught with cast net in the East China Sea near Zhoushan,and the cultured population was artificial breeding offspring of the wild population which was the first filial generation(F1).Eight EcoR I and Mse I primers combination produced 505 clear bands,of which 369 bands were polymorphic loci.The overall proportion of polymo...
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LIU Yong-shi , ZANG Wei-ling , HOU Wen-jie , ZHANG Yu , YANG Ming , DAI Xi-lin , DING Fu-jiang(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .Shanghai Shencao Special Fisheries Development Co. , Shanghai , China)
2010(2).
Abstract:通过室内60 d罗氏沼虾幼虾养殖试验,研究了不同养殖密度(150 ind/m3、400 ind/m3、550 ind/m3、800 ind/m3)对水质与幼虾生长特点的影响。试验发现,各密度组水质指标pH、DO、TAN、NH3-N、NO2--N和CODMn均控制在罗氏沼虾生长的适合范围之内,密度对主要水质指标未产生显著性影响;不同养殖密度对幼虾生长指标产生不同程度的影响,最低密度组成活率和增长率与其他各组存在显著性差异(P<0.05),次低密度组增长率与最高密度组存在显著性差异(P<0.05),最低密度组的增重率与其他各组存在极显著差异(P<0.01);4种密度组的罗氏沼虾幼虾体重(w)和体长(L)之间的关系都符合幂函数方程:w=aLb(a的范围为0.013 2~0.015 1,b的范围为3.433 0~3.502 1);体长(L)和养殖天数(d)间符合线性相关:L=ad+b(a的范围为0.038 5~0.049 2,b的范围为1.801 1~1.886 1)。室内高密度养殖罗氏沼虾幼虾可据自身养殖条件,参考150~400 ind/m3选用布苗密度。
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SUN Li-hui , WANG Ji-ying , DING Li-yun , CUI Li-jiao , SHUAI Ji-xiang , SUN Yong-zhi , ZHANG Li-min(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .Marine Fisheries Research Institute of Sh , ong Province , Yantai , China)
2010(2).
Abstract:A study was conducted to assess the effects of different feeding frequency(1,2,3 times per day and 2 times per day but Sunday) on growth performance,feed utilization,body and tissue composition of juvenile Starry Flounder(54.52 g).The results showed that weight growth rate,specific growth rate and feeding rate were enhanced significantly by feeding frequency(P<0.05).Weight growth rate and specific growth rate of 2 times per day but Sunday was significantly higher than 1 time per day(P<0.05) but no significa...
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HAN Bo , SONG Li-ping (.College of Fisheries , Life Sciense , Shanghai Ocean University , Shanghai , China , .Fresh Water Fishery Institute of Sh , ong Province , Jinan , China)
2010(2).
Abstract:To examine the effects of dietary starch on the growth performance and digestive encyze actirities of Hephaestus fuliginosus,diets containing iso-nitrogenous,iso-lipidic but increasing starch levels(26%,30%,34%,38% and 42%) were used to feed experimental fish for 60 days.At the end of the experiment,the Hephaestus fuliginosus fed 34% starch level showed higher relative weight and protein efficiency ratio,lower feed conversion ratio than other groups(P<0.05),higher level of crude lipid compositions of whole ...
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MENG Qing-jun , LIN Shao-zhen , XIANG Bin-bin , NAN Chun-rong(.Marine Science Research Institute , Marine Science Department , Wenzhou Medical College , Wenzhou , China , .Zhejiang Marine Culture Research Institute , Wenzhou , China)
2010(2).
Abstract:实验室条件下,研究了N、P浓度、不同化合态N及N∶P比值对坛紫菜(Porphyra haitanensis)N、P吸收速率,以及P浓度对坛紫菜生长速率和藻红蛋白含量的影响。结果表明,随着营养盐浓度的升高,坛紫菜对N、P的吸收速率也随之增高,当无机氮浓度达到100μmol/L时,坛紫菜对N、P的吸收速率趋向接近最大值;当NO3--N∶NH4+-N比值为1∶5时,坛紫菜对N的吸收达到最大值;坛紫菜对P的吸收速率随NO3--N∶NH4+-N比值的减小而略有增大;坛紫菜对N的吸收速率随着N∶P比值的增大而增大,而对P的吸收速率随着N∶P比值的增大而减小;在磷浓度低于12μmol/L的情况下,坛紫菜的生长速率和藻红蛋白含量随着P浓度的升高而增加,高于12μmol/L时,则不再增加。
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TONG Chun , CAO Zhen-jie , YANG Ling , QING Yu-guang , SUN Dong(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .Fresh Water Fishery Institute of Sh , ong Province , Jinan , China)
2010(2).
Abstract:配制添加不同浓度壳聚糖(0、0.2%、0.4%、0.6%、0.8%、1.0%)的6组实验饲料,网箱养殖淡水白鲳(Colossoma brachypomum)(82.26±5.13)g 60 d,探讨壳聚糖对淡水白鲳生长、非特异性免疫功能的影响和其在饲料中的适宜添加量。结果表明:0.6%组末体重、特定生长率、饲料效率和蛋白质效率显著高于0、0.2%、0.4%和1.0%组(P<0.05);0.6%、0.8%和1.0%组脏体比显著低于0、0.2%和0.4%组(P<0.05);0.6%、0.8%和1.0%组肝体比显著低于0组(P<0.05);0.6%组溶菌酶活性最高,显著高于0和0.2%组(P<0.05);0.6%组超氧化物歧化酶活性最高,显著高于其它组(P<0.05);0.6%组酸性磷酸酶活性显著高于0和0.2%组(P<0.05);用嗜水气单胞菌对淡水白鲳进行攻毒后的14 d内,0.6%、0.8%和1.0%组的死亡率显著低于0组(P<0.05)。综合各项实验指标,淡水白鲳饲料中添加适量的壳聚糖能提高其生长性能、饲料利用率、非特异性免疫功能以及抗嗜水气单胞菌感染能力,适宜添加量0.6%~0.8%。
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HUANG Lin-bin , YAN Xing-hong(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .Key Laboratory of Exploration , Utilization of Aquatic Genetic Resources , Ministry of Education , China)
2010(2).
Abstract:A strain of pathogenic bacterium was isolated from the blades of wild type Porphyra haitanensis with red-rotting disease collected from the wild population along the coasts of Pingtan Island of Fujian Province.The bacterium had a 100% similarity with Cobetia marina(Oceanospirillales,Proteobacteria) according to analysis of the 16S rDNA array.After the pathogenic bacterium was cultured in liquid medium for a period of time,an amount of bacterial suspension was inoculated onto the blades of different Porphyra...
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CAO Jie , CHEN Xin-jun , , LIU Bi-lin , TIAN Si-quan , QIAN Wei-guo , (.College of Marine Sciences , Shanghai Ocean University , Shanghai , China , .Key Laboratory of Shanghai Education Commission for Oceanic Fisheries Resources Exploitation , .Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources , Ministry of Education , China)
2010(2).
Abstract:Over the past decades,with the decline of economic finfish stocks around the world,the short-life cycle squid has become one of important fishing targets and its total catches keep on increasing.Becasue the short-life cycle squid is sensitive responding to environmental changes with great abundence fluctuations,which sometimes reduces the recruitment to the stocks,the assessment and management do not work well based on traditional population dynamic models.In this paper,we review the development of methods ...
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TIAN Si-quan , , CHEN Xin-jun , (.College of Marine Sciences , Shanghai Ocean University , Shanghai , China , .Key Laboratory of Shanghai Education Commission for Oceanic Fisheries Resources Exploitation , .Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources , Ministry of Education , China)
2010(2).
Abstract:CPUE standardization is a role in fisheries stock assessment.Different calculating methods for nominal CPUE can affect standardized CPUE which usually is used to index fisheries abundance.In CPUE standardization modeling,nominal CPUE is as a response variable which usually can be calculated in three methods:(1) for every record,CPUE is calculated by catch dividing the corresponding effort,and the all CPUEs were input the model;(2) all fisheries data records are first grouped by one spatial scale(fishing gri...
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HUANG Kang-ning , HUANG Shuo-lin(College of Marine Sciences , Shanghai Ocean University , Shanghai , China)
2010(2).
Abstract:In recent years,with the environmental degradation,resource damage,the frequency of disasters and population increasing,the coastal zones suffered the growing pressure.As an effective mechanism for ocean management,integrated coastal zone management(ICZM) is accepted by most countries increasingly,including China.In this paper,the concepts and the features of ICZM are introduced,then the current status and problems of ICZM are analyzed from three aspects.At last,based on the ICZM of USA,four relevant measur...
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TIAN Qian-tao , HUO Yuan-zi , ZHANG Han-ye , LI Xin-shu , FENG Zi-hui , WANG Yang-yang , ZHANG Yin-jiang , HE Pei-min(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .East China Sea Fishery Research Institute , Chinese Academy of Fishery Sciences , Shanghai , .School of Marine Science , Technology , Huaihai Institute of Technology , Lianyungang , China)
2010(2).
Abstract:研究了浒苔(Enteromorpha prolifera)和条浒苔(Enteromorpha clathrata)在室内条件下的生长及其氨氮吸收动力学特征。结果表明:浒苔和条浒苔藻体体重日生长率分别为17.30%和16.82%;浒苔和条浒苔藻体幼苗在温度25℃和光照140μmol/(m2.s)的条件下达到最大体长日生长率,分别为78.9%和82.1%。在1~10 g/L密度范围内,浒苔和条浒苔对NH4+-N的吸收速率随密度和时间的增加而增加,当藻体密度为10 g/L时,NH4+-N浓度分别下降了86.43%和84.13%。两种浒苔对NH4+-N吸收速率与介质中NH4+-N浓度呈显著的线性关系,在NH4+-N浓度为400μmol/L时,30 min后浒苔和条浒苔的吸收速率分别为421和409μmol/(gDW.h),说明两种浒苔吸收氨氮的方式以被动扩散为主。在400μmol/L的起始浓度下,浒苔和条浒苔对NH4+-N的吸收速率随时间变化呈现3个不同阶段:在75 min内呈快速吸收阶段,75~185 min为内部NH4+-N控制的吸收阶段,250 min后为外界NH4+-N浓度控制的吸收阶段;在700 min后,...
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FAN Zhi-feng , LI Ping , WANG Li-qing , JI Gao-hua , QIU Xue-mei(.College of Fisheries , Life Science , Shanghai Ocean University , Shanghai , China , .Shanghai Qingpu District Irrigation , Drainage Management Bureau , China)
2010(2).
Abstract:Compound constructed wetland was applied in treating agricultural irrigation polluted water studied the removal effect on nutrients and heavy metals influenced by it.The results indicated that in the case of low pollution load,the removal rate on total nitrogen(TN),ammonia nitrogen(NH3-N),nitrate nitrogen(NO3-N) as well as nitrite nitrogen(NO2-N) in wetland system were 44.11%,18.90%,52.86% and 73.32% respectively.The removal rate on total phosphorus(TP),organic compounds(CODMn) and chlorophyll a(Chla) were ...
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YANG Tao , XIN Jian-mei , XU Qing , LUO Hong-yu(Faculty of Food , Pharmacy , Zhejiang Ocean University , Zhoushan , China)
2010(2).
Abstract:The waste scrap from the North Pacific squid processing was used as raw material.Effect of extrusion process parameters on the physical properties of extruded squid protein using a twin screw extruder was studied employing response surface methodology.Twin extrusion cooking running parameters,feed moisture,barrel temperature and screw speed were examined as input variables.Meanwhile,as output variables,physical properties of the resultant extrudates included moisture and bulk density.Results showed that moi...
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HU Guang-ming , KE Li-xin(Modern Information , Education Technology Center , Shanghai Ocean University , Shanghai , China)
2010(2).
Abstract:As information technology is widely used in colleges and universities,the security of network on campus is more and more important.However,in traditional campus network,people always focus more on the exit than the inner access.A number of network security devices are set up at the exit of the network,such as firewall,IDS,Flow Control devices,while the security of inner access is always being ignored.Almost 90 percent of campus network security affairs come from the campus.Partial paralysis of the network h...
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KE Li-xin , CHEN Jia-qi , WU Kai-jun(.School of Optical-Electrical , Computer Engineering , University of Shanghai for Science , Technology , Shanghai , China , .Modern Information , Education Technology Center , Shanghai Ocean University , Shanghai , China)
2010(2).
Abstract:SNMP protocol,NetFlow technology,multi-threading technology of PHP are introduced into performance monitoring system in this paper.A set of intelligent network monitoring system is designed and developed by collecting,storing and analyzing performance data.PHP language is used to develop the system.It is divided into six modules,such as data acquisition,performance databases,data analysis and processing,as well as data display,threshold settings and warnings and user management.Different modules achieve dif...
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WANG Xing , LU Yi(Editorial Office for Journal of Shanghai Ocean University , Shanghai , China)
2010(2).
Abstract:With the construction goal establishment of high-level characteristic university,journal should still play a role of academic orientation of teaching and research and a supporting role in scientific development,and must explore a new way of development.Journal should relate the practicality of university,from the beginning of publication management concept innovation,seize the opportunity,change the idea;the innovation of the content structure should maintain the advantage in characteristics,as well as pay ...
