Abstract:运用Sybr Green Ⅰ荧光定量RT-PCR方法和放射免疫方法（RIA）分析了外源性皮质醇（cortisol）对异育银鲫促甲状腺激素β亚基（TSH-β）mRNA表达和血清甲状腺激素水平的影响.实验设计为10周,实验组每两周注射一次0.2 mg/kg体重的cortisol（用57%酒精溶解）,对照组注射同剂量的57%酒精.实验期间没有投喂饲料.在本实验条件下,研究结果显示：注射3次外源性cortisol后,异育银鲫TSH-β mRNA表达量与对照组相比略有下降;注射5次后,与对照组相比显著降低（P＜0.05）.另一方面,RIA结果显示：注射3次外源性cortisol后,实验组与对照组相比,血清甲状腺激素T3和T4含量虽略有升高,但无显著差异;而注射5次后显著升高（P＜0.05）.以上结果提示：外源性cortisol能够影响异育银鲫脑下垂体-甲状腺轴.

Abstract:采用RT-PCR法和SMART RACE（rapid amplification of cDNA ends）技术从南方鲇肝脏RNA克隆获得Hsc70基因的全长cDNA（scHsc70 cDNA）.此cDNA全长2384 bp,其中,5＇＇非编码区194 bp,3＇＇非编码区249 bp,编码区域（coding sequence,CDS）长度为1941 bp.根据编码序列推导出相应的646个氨基酸,与其他脊椎动物Hsc70序列进行同源性比较,发现南方鲇Hsc70与欧洲银鲫（Carassius auratus gibelio）、鲦鱼（Pimephales promelas）、人（Homo sapien）和鸡（Gallus gallus）的Hsc70相似性分别为96.0%、95.5%、94.9%和93.8%,表现出较高的保守性.序列分析发现scHsc70 cDNA编码的氨基酸序列中含有2个HSP70家族的特征模体并具有Dnak特征性基序（DLGTY-S-V）.南方鲇Hsc70基因的克隆为进一步深入研究南方鲇抗逆机理以及指导南方鲇的遗传选育和遗传改良都具有重要的理论意义.

Abstract:Changes of hormonal levels during seawater acclimation in Amur sturgeon (Acipenser schrenckii) were studied.Serum prolactin levels decreased continuously with the growing salinity.Serum cortisol levels in Amur sturgeon decreased after transfer from fresh water into seawater in the beginning.Serum cortisol levels in Amur sturgeon increased after transfer into 25 salinity.Serum insulin-like growth factors-I levels changed slightly during seawater acclimation.Serum thyroxine and triiodothyronine in Amur sturgeon increased slightly.Therefore these hormones acted together to promote seawater adaptation in Amur sturgeon.However,prolactin and cortisol were most important.It was feasible to culture Amur sturgeon in seawater after seawater acclimation.

Abstract:A total of 1 915 individuals of Liza haematocheila larvae and juveniles were collected with a plankton net(80 cm mouth-diameter,0.5 mm mesh-apesture) by way of horizontal trawl in 19 coastal stations of the South Yellow Sea from May 22 to 28,2005.The body-length ranged from 2 to 23 mm and those of 4 to 5 mm took the most dominance.Among these stations,the most dominant individuals were flexion larvae,accounting for 58.8%,and then preflexion larvae(25.4%),postflexion larvae(15.4%) and juveniles(0.4%).The highest average density was at St.4(243.3 ind./100 m3) and the lowest was at St.2(0.2 ind./100 m3),and others were from 0.2 to 35 ind./100 m3.The developmental stage showed the gradually increasing tendency from north to south area.In addition,the developmental stage in outer area was relatively earlier than in coastal area.These results revealed the possibility of the moving tendency toward the coast along with the current.It was necessary to protect the coastal area in order to maintain the resource recruitment of this species.

Abstract:在基础饲料中分别添加2%、4%杜仲叶粉,0.1%、0.15%杜仲纯粉,饲喂规格为37±3 g的草鱼鱼种（Ctenopharyngodon idellus）,考察杜仲对其生长、非特异免疫功能的影响.经过60 d的饲养,结果表明：（1）对照组、2%杜仲叶粉组、4%杜仲叶粉组,0.1%杜仲纯粉组、0.15%杜仲纯粉组的鱼体增重率分别为315%、308%、322%、310%、342%,饲料系数分别为1.10、1.17、1.09、1.11、0.97,其中添加0.15%杜仲纯粉显著提高了草鱼增重率（P＜0.05）,降低了饲料系数（P＜0.05）;（2）对照组、2%杜仲叶粉组、4%杜仲叶粉组,0.1%杜仲纯粉组、0.15%杜仲纯粉组的血清SOD活性分别为63.03 μ/mL、88.13 μ/mL、112.2 μ/mL、97.59 μ/mL、112.0 μ/mL;溶菌酶活性分别为9.07 μg/mL、7.83 μg/mL、23.55 μg/mL、19.27 μg/mL、30.44 μg/mL.其中4%杜仲叶粉组、0.15%杜仲纯粉组显著提高了草鱼的SOD、溶菌酶活性（P＜0.05）.上述结果表明,杜仲具有促进草鱼鱼种生长和提高其非特异性免疫功能的作用.

Abstract:研究了亚铁离子、醋酸盐和双氧水三种化学因子对雨生红球藻积累虾青素的交叉影响和虾青素积累过程中藻体总可溶性蛋白的变化.结果表明,在营养盐缺乏的前提下,添加45 mM醋酸盐和450 μM亚铁离子,25 ℃,24 h连续光照,6 000 lx光强下进行逆境胁迫诱导,能够大大缩短雨生红球藻虾青素积累周期,比空白对照提前一个月使藻细胞完全变红（显微观察）.但诱导过程中会造成部分藻细胞白化,自溶或破壁死亡,虾青素产量比空白对照下降了7.4%;在雨生红球藻虾青素积累过程中,藻体总可溶性蛋白含量逐渐降低,说明雨生红球藻中虾青素的积累可能以蛋白质的消耗为基础.

Abstract:池塘养殖斑节对虾在卵巢发育成熟过程中,卵巢和肝胰腺中的EPA（20∶5n-3）的含量呈下降趋势,在产卵后恢复到未成熟期的水平.18∶2n-6和20∶4n-6呈现一定幅度上升,分别在成熟期（Ⅴ）达到最高.18∶1n-9在肝胰腺中呈现一定幅度上升,而在卵巢中则下降.DHA（22∶6n-3）在卵巢中呈现波动,成熟期最低;肝胰腺中相对稳定而轻微下降.随产卵次数增加,卵中的16∶0、总的饱和脂肪酸（SFA）的含量及DHA/EPA的比值呈上升趋势,18∶2n-6,20∶4n-6,22∶6n-3及总的n-6含量保持相对稳定,而18∶1n-9,20∶5n-3,22∶5n-3及总的n-3含量呈下降趋势.分析结果表明,n-3、n-6系列脂肪酸,特别是DHA、EPA、18∶2n-6、20∶4n-6对卵巢和幼体的发育非常重要,不同脂肪酸之间的平衡也很重要.在斑节对虾亲虾饲料中应提供丰富的高度不饱和脂肪酸,并保持适宜的n-3 /n-6、PUFA/SFA、DHA/EPA之比.

Abstract:根据2001年6-8月我国鱿钓船对秘鲁外海（79°～90° W、5°～16° S）茎柔鱼资源的调查,对其胴长组成和性成熟作了初步分析.结果表明,渔获物胴长范围为200～880 mm,优势胴长为240～480 mm,约占总数的80%左右.依据胴长组成和性成熟度,调查海域茎柔鱼存在3个群体.雌雄性别比为2.52∶1.00.渔获物中,雄性个体的性成熟度高于雌性,雄性Ⅲ和Ⅳ期占总数84.3%,雌性仅占47.5%.同一性成熟阶段,雌性个体的胴长范围均大于雄性.经估算,雌性和雄性个体的初次性成熟胴长分别为374 mm、228 mm.

Abstract:以精子活力和受精率为参数,就收集精子的方法、抗冻剂的种类和浓度及冷冻程序和平衡时间对虾夷扇贝精子超低温保存效果的影响进行了研究,并进行了冻存精子与栉孔扇贝卵子的杂交实验.结果表明,肾管处收集到的精液与海水配的16%DMSO 1∶3（v/v）混合,在4 ℃冰箱内平衡5～10 min,在液氮面上方20cm、3 cm处分别停留3 min、10 min后浸入液氮保存,冻精活力在40%以上.在液氮中保存1 d、8 d、30 d、90 d后解冻,冷冻精子活力没有显著差异（P＞0.05）,但明显低于新鲜精子的精子活力.虾夷扇贝的冻精可以使栉孔扇贝卵子受精,受精率在20%以上,并能得到正常的D型幼虫.

Abstract:用高效液相色谱法同时分离检测水产品中的多组分生物胺.这些生物胺包括：色胺,章鱼胺,2-苯乙胺,腐胺,尸胺,组胺,5-羟色胺,酪胺,亚精胺和精胺.高氯酸溶液提取,丹酰氯衍生,再用氨水去除尸胺干扰峰.C18反相色谱柱,用醋酸胺和乙腈为流动相,梯度洗脱,流速为1.0 mL/min,柱温40 ℃;254 nm下紫外检测器检测.结果表明：平均回收率是79.59%～111.10%;相对标准偏差为2.6%～15.8%;方法检测限（信噪比S/N=3）：腐胺、亚精胺0.8 μg/g;尸胺、组胺、酪胺、精胺1 μg/g;章鱼胺、5-羟色胺2 μg/g;2-苯乙胺3 μg/g;色胺5 μg/g.

Abstract:对来自春季（4月份）、夏季（8月份）、秋季（11月份）和冬季（1月份）的草鱼（Ctenopharyngodon idellus）和鲢（Hypophthalmichthys molitrix）骨骼肌肌球蛋白进行提取和纯化,通过测定肌球蛋白在不同热变性温度下Ca^2＋-ATPase的变性速率常数（KD）,考察了两种淡水鱼肌球蛋白热稳定性的季节变化.研究结果表明,两种鱼肌球蛋白Ca^2＋-ATPase的活性均显示夏季鱼＞春季鱼＞秋季鱼＞冬季鱼.在35 ℃、40 ℃、45 ℃和50 ℃的热变性温度下,测定了两种鱼的肌球蛋白Ca^2＋-ATPase变性速率常数（KD）,证明了肌球蛋白的热变性符合一级反应速度方程.经35 ℃热变性的肌球蛋白的KD值显示了冬季鱼约为夏季鱼的4倍,而秋季鱼和春季鱼介于冬季鱼和夏季鱼之间,且秋季鱼的KD值略低于冬季鱼,而春季鱼的KD值略高于夏季鱼.在40 ℃、45 ℃和50 ℃的热变性温度下,对各种肌球蛋白测得的KD值的变化趋势与上述结果相似.本研究证明了淡水鱼肌球蛋白的热稳定性夏季鱼明显优于冬季鱼,而春季鱼和秋季鱼分别与夏季鱼和冬季鱼的相似.

Abstract:This study analysed the papers published in SCIE web database during the period of recent ten years of five universities: Shanghai Fisheries University,Jimei University,Guangdong Ocean University,Zhejiang Marine University,and Dalian Fisheries University.The characteristics of the papers among five universities were compared in various aspects,such as paper amount,author affiliation,cited number,subjects and published journal.The results showed that the papers embodied by SCIE of five universities have increased year by year,especially since 2000.From the subjects of papers,the dominant academic disciplines of Jime University were changed from the fisheries to basic disciplines such as math,physics and chemistry etc,and the dominant academic disciplines of Shanghai Fisheries University,Guangdong Ocean University and Dalian Fisheries University were ocean and freshwater biology,fisheries subjects,but that of Zhejiang Ocean University was computer science.As for the influence of papers,Shanghai Fisheries University and Jimei University kept being in the first rank.

Abstract:The status of fishery medicine in prevention and cure of fish and shellfish disease, status of research, management, usage of fishery drug in China were reviewed in this article, and also the problems in examination of fishery drug , and also including regulation of the management and usage .

Abstract:This paper reviewed the new techniques of molecular microbial ecology,which included two kinds of methods,and one was non-based on genomic DNA,including sole carbon source utilization patterns(SCSUP) and phospholipids fatty acid patterns(PLFA);the other was based on genomic DNA,including clone library analysis,metagenomics library analysis,guanine+cytosine(G+C) content analysis,total DNA re-association,community level-DNA cross hybridization,fluorescence in situ hybridization(FISH),DNA micro-array,denaturing/temperature gradient gel electrophoresis(DGGE/TGGE),single-stranded conformational polymorphisms(SSCP),restriction fragment length polymorphism(RFLP)/amplified ribosomal DNA restriction analysis(ARDRA),terminal restriction fragment length polymorphism(T-RFLP),ribosomal intergenic spacer analysis(RISA),randomly amplified polymorphic DNA(RAPD),and so on.Their potential applications in food industry were summarized: discovery of new strains,producing new enzymes,improving new techniques,and keeping food quality and safety.It provides new technical supports for deeper development of food industry.

Abstract:采用新型的生物素-磁珠吸附微卫星富集法与传统的放射性同位素杂交法相结合的方式,研究了草鱼（Ctenopharyngodon idella）基因组中存在的微卫星资源.结果,从1 000个细菌中,挑出90个阳性克隆,把其中45个进行测序分析,在这些序列中共含有68个微卫星核心序列,有55个含有重复次数大于5次的微卫星核心序列.其中,单一型标记49个,占72%,包括CA重复单元共22个,GT重复单元共18个,其他重复单元为9个;在72%的单一型标记中,完美单一型微卫星标记为44个,占65%.另外,混合型标记共19个,13为完美混合型;在所有微卫星标记中,CA重复类型最普遍,依次为GT、GA等.在68个微卫星标记中,有15个依据引物设计原理设计了引物.

Abstract:The variation of morphology about the glochidia of Hyriopsis curningii which went into the water parasitized in the gill of fish(yellow catfish) falled off the host fish was observed through optical microscope and scanning electron microscope.The results showed: the growth rate of the length and height of the shell and length of the intertwisting part were different.Under the scanning electron microscope,the glochidia of Hyriopsis cumingii had hook in the belly during the water,but the hook disappeared in the host fish.The glochidia had a little ridge and two thin wing which linked with the edge of belly,thick and little thornies were distributed over the ridge and wings.The silk of the feet of the glochidia disappeared and the interior apparatus formed gradually.

Abstract:Through the research into the export and foreign exchange contribution of China's aquaculture to agriculture,we find out that aquaculture plays an important role in agriculture,especially in export.In the meantime,both the H-O theory and the Engel's law inform us that our country's comparative advantage in aquaculture is relatively strong.Therefore,it is necessary to promote the export of aquatic products to develop our agriculture and then accelerate our country's economic growth.However,we are confronted with two problems in fishery exports,of which one is trade barriers and the other is the vicious competition of the same line.Therefore,it is necessary to take some measures,such as setting new industrial standards,systems of packing materials and label certification to eliminate technological barriers on fishery exports.Furthermore,we should produce more high value-added products to promote fishery exports.

Abstract:Assistant teaching web(ATW) is the reinforcement of classroom teaching.ATW could extend the time and space of teaching,achieve the gathering of multimedia teaching resources,change teaching mode in multi ways,update or open the teaching resources at any time as your wish.ATW was constructed in B/S structure with Web technique and structural design method.The whole was divided into several isolated but related subsystems and functional modules. ATW integrated different teaching resources such as teaching material,electronic teaching textbook,references and notices.It could run pictures,flash,audio & video files.The ATW is also the exchanging platform among teachers and students through answering questions,BBS and message board.The students could submit their schoolwork or exercises on ATW.The teachers could manage the information on ATW by themselves.