Abstract:采用16S rDNA特征序列PCR-DGGE法,分析了不同饵料饲养的暗纹东方的表皮、性腺和肠道中,以弧菌为主的细菌群落的组成。结果表明:(1)共鉴定出暗纹东方体内18种微生物,均归属于变形细菌的gamma亚群,其中13种为弧菌科细菌,3种为肠杆菌科细菌,2种为气单胞菌属细菌;(2)投喂鲜活鱼虾的与投喂人工配合饲料的暗纹东方肠道和性腺中的细菌组成是不同的,在性腺中分离到的所有12种细菌中,只有2种细菌是相同的,约占17%;在肠道中分离到的所有13种细菌中,有4种是相同的,约占31%。而在表皮上所分离到的11种细菌中,有7种细菌是相同的,约占64%;(3)DGGE特征条带V1、V13、V18、V14和V17所代表的细菌只在表皮组织被分离到,V6所代表的细菌仅出现在以鲜活鱼虾作为饵料的暗纹东方性腺中,V5所代表的细菌仅出现在以鲜活鱼虾作为饵料的暗纹东方肠道中;(4)DGGE特征条带V5、V8、V13、V18、V6、V14和V17所代表的细菌不能在TCBS培养基上培养,占18种被鉴定的细菌总数的39%。

Abstract:使用三角帆蚌、池蝶蚌作为亲本进行自交与杂交,获得了4群体F1。对实施手术无核插片手术后1年、2年、3年的4群体F1的养殖效果与育珠性能进行了比较。结果表明,在各年龄组育珠性能方面,反交F1>池蝶蚌>正交F1>三角帆蚌,且反交F1具有显著杂交优势;正交F1在壳长、成活率方面具有一定杂交优势,其它方面指标均低于池蝶蚌,略高于三角帆蚌。实施插片手术3年的反交F1较同龄的三角帆蚌每只蚌平均产珠重量增加31.96%,大规格珍珠(Φ>8 mm)比例增加2.71倍,成活率提高20.14%;较同龄的池蝶蚌每只蚌平均产珠重量增加14.95%,大规格珍珠(Φ>8 mm)比例增加54.01%,成活率提高10.14%。

Abstract:The cDNA encoding gonadotropin-releasing hormone(GnRH) and GnRH associated peptide(GAP) was amplified from total RNA from Oreochromis aurea pituitary glands by reverse transcription polymerase chain reaction(RT-PCR) method,and then blasted against other GnRH cDNA sequences in the GenBank.The analysis of the sequence data indicated that the coding region of the cDNA fragment,which encoded 89 amino acid redidues,is about 400 bp in size.A high homology was found with the GnRH coding region within O.niloticus,O.aurea and S.aurata.The amplified cDNA fragment was cloned into the prokaryotic expression vector,pMAL-c2x,to produce the expression vector pMAL-GnRH.The recombinant plasmid was transformed into E.coli TB1.GnRH-MBP fusion protein was obtained after the addition of IPTG into the growth media.SDS-PAGE analysis revealed that the GnRH-MBP was expressed after induction with IPTG for 4h.A protein band of 56 kD appeared on SDS-PAGE gel.It is anticipated that the fusion protein will be proved useful for further research.

Abstract:A function-checking vector,pYF3553 was constructed,GUS gene as the reporter gene,whose upstream was the fusion-promoter consisted of cis-acting elements of Lentinula edodes and a yeast Cyc1 gene mini-promoter.Two function-checking vector,whose cis-acting elements of Lentinula edodes were XG108 and XG111,were named pXG108 and pXG111.By transforming of the pXG108 into protoplasts of Lentinula edodes,transient expression of GUS activity was twice as high as the control.This phenomenon revealed the obvious expression of GUS gene and the modulation function of its upstream sequence.Transient GUS gene expression of the two vector,pXG108 and pXG111,was different.Difference existed in the transient GUS gene expression between the two cis-acting elements of Lentinula edodes,pXG108 and pXG111 resemble the expression effect in yeast of that.A detailed analysis of superiority and disadvantage for the method utilizing the transient GUS gene expression to detect the unknow function of cis-acting elements of edible fungi was also brought forward.

Abstract:2004年3-8月在长江口沿岸碎波带5个站位用小型拖网(1 m×4 m,网目1 mm)采集到鱼稚鱼754尾,平均密度为10.9尾/网次,以6月的出现尾数最多,5月份最少。平均密度以St.2最高(80.6尾/网次)。通过研究St.2鱼稚鱼的平均体长及生长规律,其平均体长呈逐月递增趋势,表明该鱼以沿岸碎波带作为其保育场。对205尾鱼稚鱼的耳石日生长轮的观察结果,其日轮数与体长呈对数相关,并由耳石日轮数推算出其为3-6月孵化的个体。孵化后约20~46 d的个体在沿岸碎波带水域进行短期生活。鱼稚鱼在保育场的生长规律揭示了在进行海岸工程时必须强调对生态环境和水生生物保护的重要性。

Abstract:根据1997-2000年东海23°30′~33°00′N1、18°30′~128°00′E海域4个季节海洋调查资料,运用生态学指标,探讨了东海有尾类种的组成、种数季节变化和平面分布、多样性指数(H′)变化。结果表明:本次调查共记录东海有尾类6种,其中异体住囊虫(Oikopleura dioica)、长尾住囊虫(O.longicauda)和红粒住囊虫(O.rufescens)是东海的常见种,中型住囊虫(O.intermedia)也较为常见,梭形住囊虫(O.fusiformis)和Oikopleura.Spp.则是稀有种;除了Oikopleura.Spp.,其他种在4个季节均有出现,种类季节交替不明显。异体住囊虫有较高的出现率,对环境有广泛的适应;长尾住囊虫也有广泛的分布,但其出现率明显低于异体住囊虫,与异体住囊虫相比,其更有暖水种的特征。红粒住囊虫出现的海区具有高温高盐的特征,可以认为该种是暖水种。中型住囊虫主要在夏季出现,也是暖水种。东海有尾类以分布在外海暖流势力控制的水域为主,显示出该类浮游动物具有暖水性的特征。东海有尾类多样性指数(H′)值较低,个别优势种的集群性,是形成多样性指数值较低的主要原因。

Abstract:在长江南通如皋江段和安徽安庆江段分两个时期野外采集刀鲚样本，通过对其性腺发育的观察发现：长江刀鲚种群无论是从体长还是体重方面都呈下降趋势，越靠近上游的个体越小。刀鲚性腺成熟程度与体长、体重等生物学指标没有必然联系，与所处长江江段关系不大，而与洄游时间及水温度密切相关，4月洄游群体性腺发育程度分别为Ⅱ、Ⅲ期，6月上旬洄游群体的性腺发育程度为Ⅳ、Ⅴ期。越晚洄游进入长江的刀鲚，其产卵场可能越处于下游，越接近长江口。

Abstract:28℃水温下,实验室内比较观察了盐度为0、3、69、、152、0、30、40八组水体中石磺(Onchidiumsp.)受精卵的胚胎发育状况,并对囊胚期的胚胎进行了低盐度(0、3、6、9)培育试验。结果表明:石磺受精卵在不同盐度水体中的胚胎发育状况有很大的差异,盐度0组中的胚胎畸形率高达97%±2%,盐度3组中的胚胎畸形率达75%±2%,盐度30组中停止发育的胚胎(即单卵黄球胚胎)比例为60%±4%,盐度40组中停止发育胚胎的比例达98%±2%,盐度6、91、5、20组的胚胎能正常发育,孵化率均在90%以上;石磺卵群孵化的适宜盐度范围为6~20。囊胚期的胚胎适应低盐度的能力稍强,盐度0组中的囊胚期胚胎分裂球解体,不能正常发育;盐度3、6、9组中的囊胚期胚胎能正常发育。盐度为23.4和27.4的自然海水环境中,石磺胚胎也能正常发育和孵化。

Abstract:在16L：8D和自然光N（Amb）两种光周期条件下养殖黄金鲈鱼，观察其体重增长及饲料转化情况。养殖试验结果发现经过5个月的饲养，黄金鲈鱼在16L：8D的光周期下鱼体总重量的净增量是自然光周期下的2．90倍，饵料系数比自然光照条件下低54．8％。结果表明适当延长光照时间可以促进黄金鲈鱼的生长，降低饵料系数。

Abstract:Research on the current situation of fish resources in Ulungur Lake.The study shows: There are 7 species of native fish,after transplanting by introducing species and filling the lake with water,the species were increased to 22 among them,18 species are the economic fish and the superior species is transferred from Leusiscus leusiscus baicalensis(Dybowski) to Hypomesus olidus(Pallas).During the past 40 years,the annual fish production has been fluctuated 800-4400 t.With the exacerating of human activities,the water surroundings have been changed and the fish resources have lost its balance,especially,the superior native fish resources have an invisible decline.Therefore,to strengthen care of the fish by law,the science and the technology encouraging the fish and lower catch strength,protect the fishery water environment,change to depend on the nature to multiply greatly only to add the resources,science leads to transplantation,making use of fish to produce potential well,constructing high-quality fish to grow the quality resources database,the value processes to protect the fresh industry,developing the recreational fishery etc,counter plan,attainability the fishery resources of that lake can keep on to develop the target.

Abstract:To examine the effect of stocking and management models on growth and yield of freshwater pearl mussel,Hyriopsis cumingii,an enclosure experiment was carried out in the Science and Technology Service Center for Freshwater Pearl Industry of Zhejiang Province at Fengqiao,Zhuji,from July 28 to October 28,2004.Three types of stocking including monoculture of the pearl mussel,polyculture of the pearl mussel and gibel carp(Carassius auratus gibelio), and polyculture of the pearl mussel,silver carp(Hypophthalmichthys molitrix) and bighead carp(Aristichthys nobilis) were established.For each type of stocking,two types of management including fertilizing and the combined use of fertilizer and formulated diet were used.At the start and end of the enclosure experiment,shell length,shell width,total weight of the pearl mussel,and body weight of the fish were measured.Yield of the pearl mussel and fish was calculated based on survival and growth of the pearl mussel and fish.Results of the experiment show that growth and yield of the 1 year-old and 2 year-old pearl mussel are closely dependent on the stocking model used,while the management model examined does not affect growth and yield of the pearl mussel significantly.By using polyculture,yield of both the pearl mussel and fish can be multiplied.Further comparison shows polyculture with gibel carp results in relatively good growth of 1 year-old mussel,and polyculture with silver carp and bighead carp is helpful to enhance growth of 2 year-old mussel.

Abstract:The survey on the resources of benthic algae was carried out in Naozhoudao in spring from 2003 to 2005.The composition and biomass of them were studied,and the ecological distribution sections of every alga were summarized.The results are as follows:1.56 genera,87 species of them were found.They are identified as: 3 genus,4 species in Cyanophyta;14 genera,27 species in Chlorophyta,32 genera,44 species in Rhodophyta,7 genera,12 species in Phaeophyta.2.The number of species in Tanjing was the most.3.The quantity and distributing of species among months were different.4.On the average biomass in Tanjing was the most in Mar.;Dalang was the most in Apr.,and Cunliang was the most in May.5.The algae were chiefly distributed in middle intertidal zone and lower intertidal zone.

Abstract:探讨了酶法与Sevage法联用脱除紫贻贝（Mytilus edulis linnaeus）多糖蛋白。通过正交中心组合实验，应用响应面分析法考察酶法脱蛋白的工艺条件。结果表明，酶法脱蛋白的最优工艺参数为：pH7．1，枯草杆菌中性蛋白酶加入量0．5U／mg粗多糖，温度41℃，氯化钙加入量7．4mg／g，时间3h。在酶解的基础上应用Sevage法脱除游离蛋白，适宜的工艺条件为：氯仿：正丁醇=5：1，料液比4：1，振摇时间20min。酶法与Sevage联用法脱除贻贝多糖蛋白的脱除率为78．5％；与其它传统脱蛋白方法比较，该法适应范围广、样品损耗少、经济、快速、效果理想，为多糖的分离纯化研究提供了有益的参考。

Abstract:Recently,computational fluid dynamics(CFD) has been used extensively in many fields of industry along with the development of the computer science and fluid mechanics theory.In this paper,the unsteady numerical simulation in the doorway was done by CFD,the temperature field in the doorway of a minitype cold store was analyzed.The space of the influence on the cold store by the environment was also discussed.The unstesdy temperature field in two 20s' periods of opening and closing door was simulated,it's found that the natural flow caused by the density differences is the major flow of the air around the door.It was also found that the temperature field in the downside recovered quickly,but that in the upside was not as fast as it.It needs 20 seconds to get back the initial condition.The result shows that the simulation can reflect the reality well.

Abstract:The complex working status requires construction machinery having higher reliability. Using the interrelationship between fault phenomena and fault causation, and display simulation bar on LCD, sounding and lighting Mann, the fault tree of gear shift and engine of construction machinery was established on the basis of the expert system. A remote network monitoring and diagnosis system based on Virtual Instruments is presented and the method of remote network monitoring and fault diagnosis based on LabVIEW is given. The functions of fault returning checking, fault re-displaying, fault inquiring, and LCD auto-adjusting are carried out. The research result will be an effective measure of improving system reliability of fault diagnosis of construction machinery.

Abstract:OA system is one of key technology for college information construction and has important significance. Based on the real demand of college working on digital campus, the functions for college OA system are analyzed, such as official document processing, meeting management, information publishing, individual office, schedule management of leader, and transaction processing. According to the features of OA system, the system design is selectively summarized, including the design of system structure, design of security, design of compatibility. According to the actual application in college, some typical techniques in the implementation of college OA based on web are simply introduced, such as workflow, security, vestige reservation, electronic seal and office reminder.

Abstract:Offshore fish cage becomes an important marine culture facility because it has a relatively large volume to provide sufficient culture capacity with special designed structure to withstand strongerwave,flow and wind forces at open water areas.This paper gives an overview of classification methodology of cages based on their working mode,structures,material and performances,etc.Their structures,mechanical properties,ability of survival in stronger wave and current have been discussed on the 17 selected types of cages which are currently used in marine aquaculture around the world.The results indicated that,according to their working mode,the varieties of floating type cages are the most with 52.9%; According to the cage structures,the gravity type and self-tensioned-and-self-supporting type cages account for 52.9% and 41.2% respectively;According to the flexibility of frame,the cages can be classified as the flexible frame(or non-rigid frame) and the rigid frame,their proportions are about the same;According to frame material quality,the metal cages are the most widely used up to 52.9%.In those,the gravity cages show good performance and are widely used in fishery due to simple structure,lower costs,easy operation,however,higher tension existing and concentrated on part of the netting or meshes,which might cause netting tearing and the reduction of volume of cage due to stronger current etc.,will bring about limitation on the application of gravity cages in the open water area with strong current.The rigid self-tensioned-and-self-supporting cages have advantages having a stable operation platform and easy to apply modern automatic technology or facilities.It can be expected that the rigid self-tensioned-and-self-supporting cages will be widely applied to cage culture while new material and technology are introduced.

Abstract:运用物理、化学及生物法对罗氏沼虾育苗废水进行处理。结果表明：育苗废水经物理法（沉淀、过滤）处理后，水质状况得到改善。浑浊度平均下降68．1％，NH3-Nt、CODMn平均下降24．2％与14．4％；细菌总数、弧菌数下降68．6％和75．4％。24h后二氧化氯3．0mg／L为最佳消毒浓度；24h后溴氯海因3．0mg／L为最佳消毒浓度；48h后百杀迪1．5mg／L为最佳消毒浓度。废水经臭氧消毒14h后对细菌的杀灭率达到92．7％，对弧菌的杀灭率在5h的时候便达到了100％，氧化还原电位提高了约18．6％。消毒剂净化废水的能力依次为臭氧〉百杀迪〉溴氯海因〉二氧化氯。到一定时间当生物膜成熟后，生物滤器对水质净化有良好的效果，系统可较好保持NH3-Nt低值，而保持NO2^--N低值不易。

Abstract:Two experiments were conducted to investigate the effect of coated amino acids.In Exp.1,crystal Lys,Met were coated with hardened lipid or gelatin,and the leaching loss in the water were 57%,14.3%,21.3% for crystal,hardened lipid coated and gelatin coated Lys,and 87.7%,19.0%,28.0% for crystal,harden lipid coated and gelatin coated Met.So the leaching loss of amino acid was significantly reduced by coating techniques.In Exp.2,0.23% Lys and 0.09% Met of crystal,hardened lipid coated and gelatin coated styles were added into basal diet(control group) containing 9% fish meal,to feed allogynogenetic crucian carp fingerling with body weight of(2.48 g.) After 6 weeks feeding,the growing rate of the four groups were 169.8%,173.3%,201.4%,200.2% for control group,crystal,hardened lipid coated and gelatin coated amino acids respectively.There was no effect of adding crystal amino acid on growing performance,but growing rate was improved by 18.6%,17.9%(P<0.05) and feed coefficient was decreased by 0.28,0.37(P<0.05) by adding hardened lipid or gelatin coated amino acid.Adding crystal or coated amino acid had no effect on muscle composition.Results above showed that the amino acid availability is significantly improved and the leaching loss in the water reduced by coating with hardened lipid or gelatin.

Abstract:Based on samples collected from Yichang segment of the Yangtze River,the nutritional composition in muscle of Coreius heterodon was analyzed.The results showed as follows: The flesh ratio of C.heterodon was(68.13%;) the content of moisture in muscle was 69.58%;the content of crude protein,crude fat,ash,Ca,and P in dry matter was 72.98%,17.10%,5.14%,0.64% and 1.14% respectively.In dry matter the total content of amino acids was 62.27%;the percentage of essential amino acids,delicious amino acids in total amino acids was 42.90%,44.05% respectively.The essential amino acids index(EAAI) of C.heterodon was 71.23.

Abstract:分别以羧甲基纤维素和淀粉为壁材，利用冷冻干燥法在不同的工艺条件下制备得到恩诺沙星微胶囊制剂。缓释试验表明：以羧甲基纤维索和淀粉为壁材的恩诺沙星微胶囊均表现出明显的缓释作用，且羧甲基纤维素的缓释性能优于淀粉，冷冻温度的降低能促进微胶囊缓释性能。其中，恩诺沙星原料药在4．5min时的溶出率达到99．4％。-40℃和-50℃条件下制备的以羧甲基纤维素为壁材的恩诺沙星微胶囊在14h的溶出率分别为99．2％和99．7％；-40℃和-50℃条件下制备的以淀粉为壁材的恩诺沙星微胶囊在12h的溶出率为99．4％和99．6％。

Abstract:For the implementation of the function of dynamic data exchange by different users under Windows and dynamic display and monitoring, the data from GPS receiver was displayed on the animation layers of MapX through serial interface communication controller provided by VB. This technology was applied in the minitoring of fisheries resources and the track of monitoring ship on the animation layers was converted into region through programming. The traipsed area was precisely calculated and fisheries resources was precisely estimated.

Abstract: