• Volume 0,Issue 3,2002 Table of Contents
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    • Study on digestive enzymes of Carassius auratus glibelio

      2002(3):193-198.

      Abstract (2653) HTML (0) PDF 199.77 K (1970) Comment (0) Favorites

      Abstract:对处于不同生长阶段银鲫的蛋白酶、脂肪酶、淀粉酶、纤维素酶的活性分布进行了初步研究。结果表明:在鱼苗、鱼种、幼鱼、成鱼和亲鱼5个生长阶段,胰蛋白酶和脂肪酶活性基本保持不变,纤维素酶活性呈上升趋势,淀粉酶活性从鱼苗到成鱼不断提高,但在亲鱼阶段降低。胰蛋白酶和淀粉酶活性分布均以中肠最大,纤维素酶活性则在肝胰脏中最大。在肝胰、前肠、中肠、后肠,胰蛋白酶的最适pH值分别是7.5、7.5、8.5、8.0;脂肪酶的最适pH值均为7.5。淀粉酶的最适pH值均为6.5;纤维素酶在肝胰脏的最适pH值是4.8,在前肠、中肠、后肠最适pH值均有两个峰值,分别为3.6和4.8。

    • Enzyme linked immunosorbent assay (ELISA) for the quantitative analysis of diethylstilbestrol (DES) residues in the muscle of Trionyx sinensis

      2002(3):199-202.

      Abstract (2397) HTML (0) PDF 128.18 K (1842) Comment (0) Favorites

      Abstract:根据酶标免疫法原理,用含有针对兔IgG(已烯雌酚抗体)的羊抗体与待测中华鳖肌肉中的已烯雌酚发生特异性的结合反应。洗涤后,加入的酶标底物连接未结合的已烯雌酚抗体,在酶基质的作用下与无色发色剂生成有色产物。反应后于450nm处理量吸光度,吸光强度与样品中的已烯雌酚浓度成反比。该检测方法平均最低检测下限为0.0125μg/kg,平均回收率74.8%,变异系数为1.763-3.084%。表明本方法快速、灵敏、可靠,适合对已烯雌酚残留限量检测的要求。

    • The toxicity of four heavy metals on Macrobrachium rosenbergii postlarva

      2002(3):203-207.

      Abstract (2903) HTML (0) PDF 183.17 K (2470) Comment (0) Favorites

      Abstract:探讨了Cu、Zn、Cr、Cd四种重金属离子对罗氏沼虾仔虾的急性毒性及联合毒性作用。Cu2 + 、Zn2 + 、Cr(Ⅵ )、Cd2 + 对仔虾的 96hLC50 分别为 0 .46 2mg/L、0 .5 80mg/L、0 .114mg/L、0 .0 15mg/L ;急性毒性由强至弱依次为 :Cd2 + >Cr(Ⅵ ) >Cu2 + >Zn2 + ;Cu2 + 和Zn2 + 、Zn2 + 和Cd2 + 对罗氏沼虾仔虾的联合作用表现为拮抗 ,Cu2 + 和Cd2 + 、Cu2 + 和Cr6+ 、Zn2 + 和Cr(Ⅵ )则为协同作用。

    • Spectrum properties of intact cells, phycobilisomes and phycocyanin from Se-rich Spirulina maxima

      2002(3):208-214.

      Abstract (2557) HTML (0) PDF 273.14 K (1921) Comment (0) Favorites

      Abstract:The best concentration of selenium for culturing Se-rich Spirulina maxima was studied. The results indicated that 40 mg/L Se can greatly increase the alga's growth. The spectra properties of intact cells,phycobilisomes and phycocyanin from Se-rich S.maxima and S.maxima were studied. No difference was found between them. All these results showed that selenium did not affect the absorbance and transfer of solar energy in S.maxima.

    • The growth of Nannochloropsis oculata cultured in different nutrient concentrations and the variations of nitrogen and phosphorus in culture tanks

      2002(3):215-218.

      Abstract (2718) HTML (0) PDF 117.53 K (2123) Comment (0) Favorites

      Abstract:在单胞藻培养池中 ,以f/ 2为基本培养配方 (其主要成分为NaNO3 74.8mg ,NaH2 PO4 4.4mg ,FeC6H5O7·5H2 O 3.9mg) ,在经消毒的天然河口水中 ,分别添加 1倍、2倍、3倍浓度f/ 2配方的营养盐培养微绿球藻。结果表明 ,添加 2倍营养盐浓度组 ,微绿球藻的生长最快 ,其相对生长常数显著大于添加 1倍营养盐浓度组。在培养过程中 ,水体中NO-3 N、NH+ 4 N、总氮及PO3 -4 P含量下降 ,而NO-2 N含量在培养过程中先降后升。在高浓度营养盐条件下 ,生产单位产量的微绿球藻需要消耗更多的氮肥。

    • Hydrochemistry of the coast along Caojing, Hangzhou Bay

      2002(3):219-224.

      Abstract (2529) HTML (0) PDF 160.63 K (1874) Comment (0) Favorites

      Abstract:报道了 2 0 0 1年 1- 12月杭州湾畔金山漕泾地区用于水产养殖的河口沿岸水化学状况 :总含盐量 (12 .2 4± 1.92 )g/L ,碱度 (2 .12± 0 .2 7)mmol/L ,pH值为 7.99± 0 .11,阴阳离子中含量最高者分别为Cl-(6 .6 4± 1.0 4)g/L与Na+ +K+ (3.76± 0 .6 0 )g/L ,属典型河口区水质特点 ,系海水水质类型 (ClNaⅢ)。常规水质指标如下 :Nt为 (1.32± 0 .44 )mg/L ,其中NH3 Nt占 19.7%,NO-3 N占 78.8%,NO-2 N占 1.6 %,活性磷PO3 -4 P含量为 (2 .0 4± 1.5 7) μg/L ,氮磷比 (N/P)为 6 47(原子比 ) ;化学需氧量 (CODMn)为 (6 .89± 1.76 )mg/L ;五日生化需氧量(BOD5)为 (2 .5 6± 0 .5 2 )mg/L ;溶解氧 (DO)为 (7.95± 0 .5 6 )mg/L。

    • Effects of environmental factors on density of the planktonic rotifer in Xinlin Bay Reservoir

      2002(3):225-229.

      Abstract (2175) HTML (0) PDF 153.92 K (1968) Comment (0) Favorites

      Abstract:A mechanism model simulated density dynamics of the planktonic rotifer in Xinlin Bay Reservoir.The data were based on the samples taken at 2 months intervals from October and 1999 to August 2001.The factors included ciliate biomass, phytoplankton biomass, bacteria-density, temperature (T), salinity, dissolved oxygen(DO), water transparency(SD), chemical oxygen demand(COD) and the results showed that water transparency, salinity, ciliate biomass in south part of Xinlin Bay Reservoir were the dominant factors on controlling rotifer density, while phytoplankton biomass, bacteria-density, ciliate biomass, water transparency in north part of Xinlin Bay Reservoir were the dominant factors. By using multiple linear regression and non-linear regression to the model identification,two non-linear regression models were established.

    • Primary study on some environmental behaviors of 110mAg in the West Daya Bay

      2002(3):230-236.

      Abstract (2461) HTML (0) PDF 235.99 K (1933) Comment (0) Favorites

      Abstract:mAg released from normal operation of the Daya Bay nuclear power plant had been assessed the critical radionuclide to the environment. Availability monitoring and assessment of environmental impacts depend upon comprehension of the environmental behaviors. The 110mAg contents in various environmental media involving seawater, sediment, and some marine organisms are measured in the West Daya Bay. Some environmental behaviors of 110mAg in the bay are discussed primarily. The result of screening biological indicator shows that the bivalvia and gastropod are probably appropriate objective for 110mAg environmental monitoring in the Bay. Sargassum is also available indicator in spring.

    • A primary discussion on the mode of fisheries management in USA

      2002(3):237-241.

      Abstract (2248) HTML (0) PDF 106.68 K (2031) Comment (0) Favorites

      Abstract:Fisheries management system in the United States has become more and more complete after decades of practice. In this system, fisheries legislation and fisheries law enforcement has been greatly strengthened. The core of the fisheries management work has been changed from fishery itself to management of fishery, from management of the resources and environment to the management of people. Management measures are also well supported by the fisheries scientific research. Local fishery associations have been established in order to improve the role of the fishermen in the management work. Compared with the management system in the United States, the management system in China should be improved. More budget should be invested in the fisheries scientific research. Fishery association composed of mostly fishermen should be established to improve the role of the fishermen in the management work. This paper tries to discuss the mode of fisheries management in USA, and compare that mode with the Chinese fisheries management mode; then find out some aspects which are worth learning and using for reference . Finally, it concludes with some primary suggestions and advice for the fisheries management department for reference.

    • Large scale artifical breeding of hard clam Meretrix meretrix Linneus

      2002(3):242-247.

      Abstract (2560) HTML (0) PDF 214.70 K (2101) Comment (0) Favorites

      Abstract:1998年至2001年就文蛤亲贝能肥促熟、诱导产卵、孵化、幼虫和稚贝培育、稚贝生态习性等开展研究。研究发现,养殖文蛤可以通过室内控温强化培育促熟,比自然海区提早近2个月;通过阴干-充气的催产方法,能使亲贝集中大批排放精卵。在水温26-32℃,pH7.8-8.5,盐度15-30的条件下,幼虫生长及变态最快。试验结果表明:采用无附着基质的方法进行采苗及稚贝培育是可行的;文蛤稚贝对干露、海水盐度、温度、pH、氨氮等具有较强的耐受性,适应范围:海水盐度9.6-32,水温4-34℃,pH7.0-8.5,在pH=7.9时,能忍受10mg/L的总氨氮浓度。1999年至2001年,共育出1.02-1.75mm(壳长)的文蛤稚贝2.2亿颗,单位面积出苗28.01-44.60万颗/m^2,达到产业化的要求。

    • The technology of induction tetraploid in Crassostrea gigas

      2002(3):248-252.

      Abstract (2379) HTML (0) PDF 181.33 K (1916) Comment (0) Favorites

      Abstract:报道了长牡蛎四体诱导的新技术。采用长牡蛎二倍体卵子与精子授精,用1.5mg/dm^3CB在受精后4min处理受精卵40min抑制第一和第二极体排放诱导四倍体,获得稚贝(1-3mm)2000粒,其中四倍体占85%,三倍体占5%,三倍体/四倍体嵌合体占10%。本文首次报道可存活的三倍体/四倍体嵌合体稚贝。

    • Effect of water temperature variation on reproduction of Cyprinus carpio haematopterus

      2002(3):252-258.

      Abstract (2438) HTML (0) PDF 175.11 K (1918) Comment (0) Favorites

      Abstract:抚过江段的黑龙江野鲤产卵时间为5月下旬水温上升到16-17℃后的2-3d,水温迅速上升是产卵的刺激信号。在低温(11-15℃)环境中进行人工催产,保持水温稳定或上升,催产率与18-20℃水温时相近。在16-20℃环境中进行人工催产,水温下降超过3℃,催产率接近0。捕获的亲鱼分别在10-11℃、14-15℃、17-18℃水温环境中雌雄分离蓄养7-30d后进行人工催产,蓄养时间越长,温度越高,雌鱼的催产率越低,雄鱼则无明显影响。黑龙江野鲤襄胚原肠期胚胎在10℃、孵出期胚胎在14℃时发育停滞。神经胚期胚胎在12℃死亡率超60%;仔鱼期幼鱼的9℃死亡率达80%。

    • Effects of different protein levels and animal protein percentage on Pelteobagrus fulvidraco

      2002(3):259-263.

      Abstract (2224) HTML (0) PDF 116.99 K (1947) Comment (0) Favorites

      Abstract:Using two-factor, five-level, orthogonal regression rotational combination design, using fishmeal as animal protein and soybean meal as plant protein, to formulate 9 diet combinations, 850 Pelteobagrus fulvidraco with an average intial weight of 11.4g, were randomly divided into 17 groups (one control and 16 treatments) in feeding trial for 68d to study the optimal levels of crude protein in diets and the optimal percentage of animal protein in crude protein. The results indicated that the optimal levels of crude protein in diets were 42.5% and the optimal percentage of animal protein in crude protein was 67%. They produced fastest growth, highest daily gain and constitute best ratio in the diet.

    • Use of inosine monophosphate and hy poxanthine as indices of quality of shrimp ( Macrobrachium rosenbergii )

      2002(3):264-267.

      Abstract (2313) HTML (0) PDF 168.65 K (2018) Comment (0) Favorites

      Abstract:测定了不同温度保藏期间罗氏沼虾肌肉组织中ATP及其降解产物含量变化,并对其鲜度进行感官评定。结果表明肌苷酸和次黄嘌呤的含量怀感官评定的结果之间有良好的相关性;IMP含量在保藏中有一积累过程,达最高值后逐渐下降;当感官评定结果表明沼虾已不可食用时,Hx的含量达0.70μmol/g以上;Hx可作为评定冷藏期间沼虾质量的一个指标。

    • The development of a campus logistic information system model based on Web

      2002(3):268-271.

      Abstract (2236) HTML (0) PDF 105.68 K (1939) Comment (0) Favorites

      Abstract:A campus logistic information system model based on Web was developed. The system has two functions; those are the release of logistic information and the feedback. HTML+ASP+VBScipt and Frontpage was adopted to develop network page. SQL sever 7 was adopted by background database, the foreground and background is linked by IIS. There are several characters in the system. The information was updated quickly, the use and management was convenient, it was economic in human and financial resource and it had high value in application because of multiply entrance.

    • A Simulation software for staring thermal imaging system

      2002(3):272-276.

      Abstract (2566) HTML (0) PDF 186.37 K (1904) Comment (0) Favorites

      Abstract:现代高科技的发展离不开系统的理论指导及计算机模拟。通过研究热成像系统原理,用VC 语言编制了一个热成像系统仿真软件包。该软件包除了可以计算热成像系统包括MRTD,MTF,NETD,MDTD,视距等的静态性能参数外,还有一个非常创新的特点:它允许用户输入原始图像,在选定的热成像系统的作用下,用户可以得到被所选定的热成像系统任何模块(如大气、光学系统,探测器、电子学等)处理过的图像,真实直观地观察各个模块对图像的影响,这对热成像系统设计指导和分析有关更深的指导意义。

    • Applications of 3S spatial information technology in marine fishery

      2002(3):277-282.

      Abstract (2546) HTML (0) PDF 134.32 K (2128) Comment (0) Favorites

      Abstract:海洋渔业面临着资源空间分布不均 ,开发强度失衡 ,渔具选择不当 ,渔船作业失控 ,海难补给与救助困难等问题。这些问题严重影响着海洋渔业的可持续发展。遥感 (RemoteSensing ,RS)、全球定位系统(GlobalPositionSystem ,GPS)和地理信息系统 (GeographicInformationSystem ,GIS)三种空间技术的出现为解决这些与空间信息相关的问题提供了可靠的技术基础。遥感提供了海洋渔业的环境信息 ,全球定位系统提供了渔船及其作业的位置 ,地理信息系统提供存储、管理、显示和分析海…

    • Advances in the study of toxin in halobios

      2002(3):283-288.

      Abstract (2594) HTML (0) PDF 216.58 K (2233) Comment (0) Favorites

      Abstract:在海洋生物中 ,存在一类高活性的特殊代谢成分 ,即海洋生物毒素。海洋生物毒素资源丰富 ,种类多 ,分布广 ,据估计有 1 0 0 0多种 ,其中已确定结构的有几十种。海洋生物毒素是海洋天然产物的重要组成部分 ,亦是海洋生物活性物质中研究进展最迅速的领域 ,海洋生物毒素具有结构奇特 ,活性广泛且活性强等特点。海洋生物毒素具有独特的药理活性 ,是典型的海洋生物药物。多数海洋生物毒素均有较强的神经毒性 ,而且作用于神经离子通道 ,因而对神经系统起重要作用。本文主要报道了河豚毒素、芋螺毒素和海葵毒素这几年的研究进展 ,其它几种毒素做了简…

    • The characterization and application of bacteriocins from lactic acid bacteria

      2002(3):289-294.

      Abstract (2407) HTML (0) PDF 216.58 K (1856) Comment (0) Favorites

      Abstract:乳酸菌素是从一般认为安全的乳酸菌产生的 ,这引起了人们极大的兴趣 ,通过它来控制食品中的某些腐败菌和病原菌。特别是乳酸菌产生的细菌素对单核细胞增生李斯特氏菌 (Listeriamonocytogenes)具有抑菌特性。乳酸菌素是具有生物活性的蛋白质 ,它对其它相近种类的细菌具有抑制作用 ,乳酸菌素一般是带正电荷的小分子蛋白 (3 0~ 6 0个氨基酸残基 ) ,具有高等电点和亲水特性[1 ] 。以前的研究主要集中于纯化 ,氨基酸顺序测定 ,遗传因子的描述等。而对它们作用机制所知甚少。根据Nettles和Barefoot的报道乳…

    • Energy efficiency retrofitting approaches for brine-way block-ice making technology

      2002(3):295-298.

      Abstract (2445) HTML (0) PDF 0.00 Byte (4) Comment (0) Favorites

      Abstract:盐水制冰是典型的间接冷却系统 ,与直接冷却系统的快速制冰工艺比较 ,盐水制冰因制冷效率低而耗能较大 ,同时盐水也容易腐蚀冰桶和其它制冰设备。但由于盐水制冰所产生的冰块密实坚硬 ,不易融化 ,易于贮藏和运输 ,所以盐水制冰目前仍然是渔业用冰的主要制冰手段。几十年的生产实践也证明盐水制冰在制冰业中的地位是其它制冰工艺难以取代的。因此探讨如何在生产实践中提高盐水制冰的效率 ,如何减少能耗 ,减少腐蚀是一个十分有意义的课题。1 盐水制冰工艺的电耗情况目前典型的盐水制冰工艺大多采用活塞压缩式制冷系统 ,蒸发温度一般是 - 1 5…

    • Layout and construction of the electronic reading room

      2002(3):299-303.

      Abstract (2395) HTML (0) PDF 123.15 K (1813) Comment (0) Favorites

      Abstract:随着计算机网络技术和光盘技术的快速发展 ,先进的Internet网和电子出版物正快步进入图书馆 ,图书馆数字化逐步由理想变成现实。信息载体与信息传输的电子化、数字化和网络化 ,使图书馆的服务功能也正经历着前所未有的变化与革新 ,大量光盘、多媒体出版物的发行和Internet网上信息的丰富 ,人类获取知识和信息的方式发生了巨大的变化 ,图书馆建立电子阅览室成为可能和必要。电子阅览室 ,以先进的电子计算机技术、多媒体技术、远程通讯技术和网络技术为依托 ,由一定数量的电脑和网络设施组成 ,具有声音、图像、图形、文字等传…

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