Abstract:本文以日本对虾为材料提取和部分纯化酚氧化酶，并对其特性进行了研究，以邻苯二酚为作用底物，该酶的最适ｐＨ为６．５，在ｐＨ５．０－８范围内较高稳定性，最稳定的ｐＨ为７．０，最适温度为４０℃，在４０℃以下表现出较高的热稳定性，而在５０℃以上迅速失活。该酶对不同的酚类物质表现出不同的底物专一性，由高至低的趋势依次为三元酚、二无酚和单元酚。米氏常数Ｋｍ值测定表明，该酶对邻苯二酚比ＤＬ－多巴有更高的亲和力。浓

Abstract:Biochemical genetic characteristics of GIFT (Genetic Improvement of Farmed Tilapia) strain, "EGYPT" strain, "78" strain, "88" strain and "AMERICA" strain of Nile tilapia (Oreochromis niloticus) were analysed by polyacrylamide gel electrophoresis.The result showed that there were significant differences of phenotype of Est in the liver among different strains. Est-2 was only found in GIFT strain and "EGYPT" strain, which could be used as a genetic marker to differentiate these strains of Nile tilapia.

Abstract:In this paper, computer aided design(CAD) technology is applied to general V-belt transmission. A number of tables and diagrams for V-belt transmission design are programmed. Furthermore, according to the maximum load-bearing capacity of V-belt, optimum technique is introduced to determine the V-belt type, standerd length, numbers and center distance of belt pulley, etc. The design parameters are inputted in man-machine conversational mode, and the operational instructions are shown on screen in Chinese. The program can be carried on an IBM-PC or 486 or 586 micro-computer. Case study shows that the technique is applicable to engineering design and computer aided instruction (CAI) for courses of machine design, Fundamentals of Machine Design and CAD in Mechanical and Optimum Design, etc.

Abstract:In modern air conditioning with ice cooling storage and district cooling, ice slurry is a kind of effective material for cooling storage and for transmitting cool thermal energy. To design the pipe system, its friction loss characteristics in pipes are very important.In this paper, a three-layer model for ice slurry flowing in horizontal pipes has been presented, and an calculation example has also been made.

Abstract:本研究用同一蛋白质和能量水平、不同脂肪和糖含量的饲料投喂试验青鱼，分别测定其在一天中氨氮、尿氮排泄量及耗氧量、CO2排出量，计算出用作能量消耗的蛋白质、脂肪和糖量。结果表明：当饲料中脂肪含量为0时，用作能量消耗的蛋白质为10．38毫克／克·日，当脂肪含量为12％时，用作能量消耗的蛋白质为5．78毫克／克·日，脂肪含量过低或过高（0或12％），均能引起饲料中用作能量消耗的蛋白质量的增加，从而导致氨排泄量的升高，分别为1．68毫克氮／克·日和0．93毫克氮／克·日，当脂肪含量为7％时，用作能量消耗的蛋白质部分最少，只有1．95毫克／克·日，氨的排泄量最低，只有0．31毫克氮／克·日。因此，饲料中适宜的能量、蛋白质、糖和脂肪含量，对蛋白质能起到较好的节约效果。

Abstract:Measurement of organic acids derived from metabolites of biologically nourishing species of bacteria in fermented soybean milk was studied in this paper. In gas-liquid chromatograpth it is very necessary to pretreat the sample due to its high content in proteins and nonvolatile solubles. Firstly, for the pretreatment the precipitation of proteins in fermented soybean milk with diatomites was carried out. Moreover, as short-chain fatty acids and their esters are highly volatile and water soluble, it is rather difficult for measurement by gas-liquid chromatograpty. Therefore, a method of benzyl esterification was adapted in the experiment. The experimental results showed high precision and good recovery. In addition, organic acids in some tonic oral liquids and yogurts were also measured.

Abstract:According to obsrevation and comparison on the sensory canal pore and the regular arrangement of sensory papillae in head, this thesis reviews the classification and makes the textual research for the records and studies of Acentrogobius. The species of Acentrogobius should be divided into two sub-genera that are AcentrogObius and Creisson. The species with a longitudinal pattern belong to the sub-genus Creisson, with longitudinal and the transverse pattern satisfactory to both sides belong to the seb-genus Acentrogobius. Then write the synonyms and classify synopsis. In China there are 11 species of Acentrogobius as follows:1. Acentrogobius (Creisson) microps Chu et Wu2. Acentrogobius (Greisson) pflaumi (Bleeker)3. Acentrogobius (Creisson) moloanus (Herre )4. Acentrogobius (Creisson) chusanensis (Herre)5. Acentrogobius (Creisson) notophthalmus (Bleeker)6. Acentrogobius (Creisson) janthinopterus (Bleeker)7. Acentrogobius (Creisson) caninus (Valenciennes )8. Acentrogobius (Creisson) chlorostigmatoides (Bleeker )9. Acentrogobius (Acentrogobius) Viganensis (Steindachner)1O. Acentrogobius (Acentrogobius) ocyurus (Jordan et Seale)11. Acentrogobius (Acentrogobius) viridiPunctatus (Valenciennes )