The invasion of Pomacea canaliculata poses a significant threat to the survival of native species and disrupts the functioning of native ecosystems. Daphnia magna， known for its rapid reproduction， malleable body shape， and ease of observation， is frequently used as a model organism in environmental risk assessment studies. This study aimed to explore the effects of the invasion of P. canaliculata on the life history， morphology， antioxidant performance， and gut microbiota of D. magna by establishing four experimental groups: no-snail control group （Ctr）， native snail Cipangopaludina chinensis group （CC）， limited-bacterial P. canaliculata group （LBPC） and P. canaliculata group （PC）. The results showed that the invasion of P. canaliculata promoted the growth， and reproduction of D. magna， and affected its life cycle. D. magna in the PC group had significantly reduced body length and tail spine length （P<0.05）， while the population size and reproductive rate were significantly increased （P<0.05）. Additionally， the activity of antioxidant enzymes was significantly enhanced （P<0.05）. Additionally， the β-diversity of intestinal microbiota in D. magna was significantly altered in the PC group （P<0.05）， with predicted microbial pathways shifting toward anti-inflammatory functions and enhanced metabolic capacity. However， inhibiting pathogenic bacteria in the intestinal tract of P. canaliculata alleviated its indirect stress effects on the growth， reproduction， and intestinal microbiota composition of D. magna. These findings indicate that P. canaliculata exerts significant indirect pressure through non-predatory interactions， affecting the physiological characteristics and gut microbial community of D. magna， potentially posing a risk to native freshwater ecosystems， which may be partly attributed to changes in its gut microbiota. This study enriches the understanding of the interspecific interaction mechanisms between P. canaliculata and native zooplankton， providing important insights into the life history strategies of P. canaliculata.