In order to compare the differences between the CO₂ fluxes at the water-air interface between the aquaculture areas and the non-aquaculture areas of Eriocheir sinensis on the lake surface and the factors affecting the CO₂ fluxes. The experiment was carried out at Luoma Lake from August 4 to August 5， 2023， where CO₂ fluxes were measured at the water-air interface between the aquaculture areas and the non-aquaculture areas of Eriocheir sinensis of the lake by using the PS-9000 Portable Automatic Measurement System of Carbon Fluxes and the synchronized measurement of the meteorological factors and water quality indices were analyzed at the same time. The results showed that the average value of CO₂ fluxes in the aquaculture area was 0.012 μmol/（m²·s）， which showed an overall CO₂ emission source， and the CO₂ fluxes in the non-aquaculture area was -0.006 μmol/（m²·s）， which showed an overall CO₂ sink. However， both aquaculture and non-aquaculture areas exhibit CO₂ as a source during the day and a sink at night. Multiple stepwise regression analyses showed that the main environmental factors affecting CO₂ fluxes differed between aquaculture and non-aquaculture areas， with air temperature and pH being the the former and total phosphorus， nitrate nitrogen and total organic carbon being the the latter. The results of the study revealed the characteristics and influencing factors of CO₂ fluxes at the water-gas interface between aquaculture and non-aquaculture areas of Eriocheir sinensis in lake， which provides data support for an in-depth understanding of the CO₂ fluxes characteristics of different aquaculture functional areas of freshwater lakes.