In order to explore the conditions for the catalytic synthesis of α-arbutin by recombinant marine sucrose phosphorylase Suc75290 from the ocean and its separation and purification conditions， sucrose and hydroquinone were used as substrates， and marine sucrose phosphorylase Suc75290 was used as a catalyst to synthesize α-arbutin. The macroporous adsorption resin HP20 was used to separate and purify α-arbutin and hydroquinone in the reaction solution. The results showed that the optimum conditions for the synthesis of α-arbutin catalyzed by marine sucrose phosphorylase Suc75290 were as follows ： MES-NaOH buffer with pH value of 7， reaction temperature of 40°C， optimum hydroquinone concentration of 38.5 g/L， optimum molar ratio of sucrose to hydroquinone of 5∶1 （ sucrose concentration of 600 g/L）， optimum enzyme dosage of 300 U/mL， and optimum reaction time of 28 h. The conversion rate of hydroquinone was 96.70%， and the content of α-arbutin was 64.3 g/L （238.7 mmol/L）. The macroporous resin adsorption method was used for separation， and deionized water was used as the loading buffer. The optimal separation conditions were determined as follows： the loading volume was 10 mL， the loading flow rate was 0.5 mL/min， the eluent was 30% ethanol， and the elution flow rate was 1 mL/min. 50 mg of α-arbutin was obtained after rotary evaporation， and the purity was 99.9%. In summary， the optimization of the conditions for the synthesis of high-concentration α-arbutin using marine sucrose phosphorylase Suc75290 and the study on the separation and purification process of α-arbutin provide an application basis for the development and utilization of high-purity α-arbutin.