To evaluate the effects of crisp culture on the growth， serum biochemical indexes and intestinal health of tilapia， Nile tilapia（Orechromis niloticus）（665±34） g were fed with faba bean， crisp feed （approximately 50% faba bean） and ordinary feed. The growth， serum biochemistry， intestinal tissue morphology， tight junction protein， digestive enzyme， antioxidant enzyme， inflammatory gene and intestinal flora of tilapia were investigated at 40 d and 80 d. The results showed that： The body mass/length and weight gain rate in the faba bean/crisp feed group were significantly lower than those in the ordinary feed group. The serum lysozyme （LYZ） in the faba bean group was significantly higher than that in the ordinary feed group. There was no significant difference in serum alkaline phosphatase （AKP） between the faba bean group and ordinary feed group. The serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） increased at 80 days in faba bean group. There was no significant difference in serum ALT， AST and AKP between the crisp feed group and ordinary feed group， whereas LYZ was significantly higher in the crisp feed group. The length of intestinal villi in the faba bean/crisp feed group was shorter than that in the ordinary feed group. The expressions of tight junction protein genes Occludin/ZO-3 /Claudin-3 decreased significantly and the expression of Claudin-12 increased significantlyin the faba bean group. There was no significant difference in the expressions of the above genes between the crisp feed group and ordinary feed group. The activities of α-amylase and lipase in faba bean group decreased， and the activity of lipase in crisped feed group decreased only at 40 d. The content of malondialdehyde （MDA） in faba bean group significantly increased， and the total antioxidant capacity （T-AOC） significantly increased at 80 d. There was no significant difference in MDA content between the crisp feed group and ordinary feed group， but T-AOC significantly decreased. The expressions of pro-inflammatory genes IFN-γ， TNF-α and anti-inflammatory gene IgM were significantly higher in the faba bean /crisp feed group than those in the ordinary feed group. The expressions of anti-inflammatory genes TGF-β1 and IL-10 decreased in the faba bean group， but the TGF-β1 and IL-10 significantly increased in crisp feed group. The ACE， Chao1， Simpson and Shannon indexes of intestinal microorganisms in the crisped feed group were close to those in the ordinary feed group but higher than those in the broad bean group. The abundance of Lachnospiraceae and Lactobacillus in the crisped feed group decreased， while the abundance of Mycobacteria and multiple Clostridium increased. The abundance of Firmicutes， Fusobacteria and Cetobacterium in the broad bean group decreased， whereas the abundance of Proteobacteria and Mycobacteria increased. Compared with 40 d， there were no significant changes in most indexes in the crisp feed group at 80 d. In conclusion， feeding tilapia with faba bean or crisp feed （approximately 50% faba bean） inhibited growth and intestinal antioxidant capacity， shortened intestinal villi， induced intestinal inflammatory reactions， reduced the abundance of beneficial bacteria and increased the abundance of pathogenic bacteria in the intestine. However， the adverse effects of crisp feed were weaker than those of faba bean. The crisp feed had no significant effect on intestinal digestive enzyme activity， cell membrane permeability and anti-inflammatory system. Faba bean had significant negative effects on the above indexes.