Isolation and characterization of a new strain with fast growth in Pyropia suborbiculata (Bangiales, Rhodophyta)
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S968.431

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    Abstract:

    Compared with Pyropia haitanensis cultivated in southern China, Pyropia suborbiculata has stronger high-temperature resistance, but its round algae are not suitable for production. Therefore, several long-type mutants were isolated from the wild-type strain of P. suborbiculata by artificial mutagenesis. In this study, the blades of the long-type mutant (CCT-2) of P. suborbiculata were induced by 60Co-γ ray, and a new strain (PS-M1) with fast growth was screened from its progeny with growth rate as the main breeding index. The PS-M1 blades cultured at 23 ℃ for 60 days had a mean length of 68.1 cm and a length-width ratio of 26.3, which were 2.7 and 2.4 times of CCT-2, respectively. The blades of PS-M1 and CCT-2 cultured at 23 ℃ for 30 days and then cultured at 30 ℃ for another 30 days, their mean length had increased by 6.4 and 3.0 times, respectively. The survival rates of conchospores of PS-M1 and CCT-2 were 47.4 and 42.2%, respectively, after 14 days of culture at 30 ℃, with no significant difference between them. The blades cultured at 23 ℃ for 45 days had a mean chlorophyll a content of 6.5 mg/g and phycobiliprotein content of 64.9 mg/g in PS-M1, which were 20.4% and 22.9% higher than those of CCT-2, respectively. The thickness of PS-M1 was 33.6 μm, which was 15.8% thinner than that of CCT-2. The total numbers of conchospores released by PS-M1 was up to 2.223 million per shell for 10 consecutive days, which was 4.8 times that of CCT-2. In conclusion, compared with long-type mutant (CCT-2), the new strain (PS-M1) of P. suborbiculata has a higher growth rate, better resistance to high-temperature and also shows obvious advantages in main pigments protein contents, and number of conchospores releasing, which has potential to be applied in commercial cultivation.

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应家轩,丁洪昌,严兴洪.圆紫菜快速生长新品系的分离与特性分析[J].上海海洋大学学报,2025,34(3):519-529.
YING Jiaxuan, DING Hongchang, YAN Xinghong. Isolation and characterization of a new strain with fast growth in Pyropia suborbiculata (Bangiales, Rhodophyta)[J]. Journal of Shanghai Ocean University,2025,34(3):519-529.

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History
  • Received:April 02,2024
  • Revised:May 03,2024
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  • Online: May 23,2025
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