Luster is the index to measure the maximum value of pearl. To study the relative contribution of Hyriopsis cumingii donor mussel and pearl mussel to the luster of nucleated pearls, white mussel (inner shell is white) is used as material, shell length and green shell are used as selection traits. According to different selection strategies, eight groups of H. cumingii populations were established. Each group was used as donor mussel and host mussel respectively for combined nuclear insertion. After 18 months of cultivation, it was found that: Among the different pearl breeding combinations, the pearl luster value of YAA pearl breeding combination from the selected population of group I was the greatest, which was 15.46% and 21.51% higher than that of YGG and YHH respectively. There was a significant difference in the luster of pearls cultivated by different groups of donor mussel. There was a very significant positive correlation between the inner shell luster of donor mussel and the luster of pearls (r=0.717-0.939). The regression equation between inner shell luster and pearl luster was established as follows: y=16.81+0.93x(R²=0.777).There was no significant correlation between the growth traits of donor mussel and the pearl luster. There were also significant differences in the luster of pearls cultivated by different groups of host mussels, but there was no significant correlation between the growth traits and luster of host mussels and the luster of pearls. The above results show that the breeding of H. cumingii with green shell color at the juvenile mussel stage has achieved the goal of improving pearl luster, which can be regarded as an indirect trait of breeding H. cumingii with high luster,the inner shell luster of donor mussel is significantly correlated with pearl luster, and the inner shell luster is better as the target trait.