In order to explore the changes of nutrients in the fertilized eggs of Australian freshwater lobster during development, the fertilized eggs in the egg-nauplius stage, the embryo with eye pigment forming stage, the prepare-hatchling stage and newly hatched shrimp were systematically analyzed in this study. The results showed that, moisture content in fertilized eggs increased significantly with the development of fertilized eggs to newly hatched shrimp, crude protein and lipid content decreased significantly with the development process, the ash content of newly hatched shrimp was the highest; Total amino acids, essential amino acids, and flavoring amino acids content of fertilized eggs decreased significantly with the development process; Based on the nutritional evaluation by amino acids score (AAS) and chemical score (CS), (Met+Cys) was the first limiting amino acids of fertilized eggs and newly hatched shrimp; EAAI of fertilized eggs and newly hatched shrimp decreased significantly with the development of fertilized eggs to newly hatched shrimp; 10 main fatty acids were detected in fertilized eggs and newly hatched shrimp of Australian freshwater lobster, and the contents of PUFA and DHA in newly hatched shrimp were significantly lower than those in fertilized eggs, but the content of EPA+DHA was significantly higher than that of fertilized eggs. In conclusion, the content of ash and EPA+DHA of newly hatched shrimp were significantly higher than those of fertilized eggs, so the addition of calcium dihydrogen phosphate and EPA+DHA can be appropriately increased in the preparation of open bait for Australian freshwater lobster larvae.