In order to explore the response characteristics of Apostichopus japonicus transplanted to the south under high temperature stress,and to determine the expression changes of heat shock protein genes hsp10, hsp60, hsp70 and hsp90 at different temperatures, we studied the activity changes of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LSZ) at different temperature. The results showed that the expressions of heat shock protein genes in the experimental group (20, 24, 28, 32℃) were different from those in the control group (16℃), especially in the high temperature groups of 28 and 32℃. The expression of each gene increased significantly. Hsp70 is more sensitive to heat stress than other genes. Under 28℃ stress, in the comparison of intestinal tissues of southern population (group S) and northern population (group N), the expression of group N decreased gradually with the increase of time, while that of group S increased at first and then decreased. Except for hsp70, the expression of other genes in group S was lower than that in group N at each time point. The activities of SOD and LSZ in S and N groups increased at first and then decreased with the increase of temperature, while the activities of T-AOC, CAT, ACP and AKP decreased with the increase of temperature. Except that the activities of T-AOC and CAT in group S were lower than those in group N at 28℃, the activities of all enzymes in group S were higher than those in group N at other temperature. The results showed that the A. japonicus population that moved south showed higher temperature tolerance after a long period of annual culture in Fujian.