Morphologic and genetic analysis of the artificially induced gynogenesis in Qingtian paddy field carp
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S917

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    Abstract:

    The resources of the indigenous common carp was exploited which are bred in paddy field to become a paddy-field carp in Qingtian, Zhejiang Province. The gynogenesis in Qingtian paddy field carp was artificially induced by sperm inactivated via ultraviolet irradiation, and followed by meiotic chromosome doubling in the method of cold shock. The gynogenesis offspring (G1) were obtained. Using common offspring (F1) as standard, the morphological characteristics and gonad developments of the 160-day-old G1 were observed. SNP technology was used for genetic identification of G1 at the genomic level. The results showed G1 had 3 types of body colors and different growth rates. Out of them, the black type shows the fastest growth, and an average body mass reached 392.45 g. The average growth rate of G1 was significantly slower than F1. G1 showed morphological proportion's difference from F1, and the ratio of total length/body depth was significantly different. In individuals, G1's ovaries developed in various periods, and ovaries of the large individuals had predominantly primary oocytes in stage Ⅲ which was similar to F1. For the purity rate of genetic material of paddy field carp by SNP detecting, the average rate of homozygous loci 61.85% of G1 was higher than 38.42% of maternal genome through gynogenesis technology, and some paternal genetic materials integrated into G1's individuals, resulting in the differences in body colors and growth rate. G1 will be used for the conservation of genetic resources, and further for the selected breeding of Qingtian paddy field carp.

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支宇,刘其根,吴嘉敏,刘东.人工诱导青田田鱼雌核发育子代形态和遗传分析[J].上海海洋大学学报,2022,31(4):839-848.
ZHI Yu, LIU Qigen, WU Jiamin, LIU Dong. Morphologic and genetic analysis of the artificially induced gynogenesis in Qingtian paddy field carp[J]. Journal of Shanghai Ocean University,2022,31(4):839-848.

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History
  • Received:April 30,2021
  • Revised:September 27,2021
  • Adopted:September 27,2021
  • Online: July 22,2022
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