In order to further improve the quality of crude large yellow croaker liver oil and remove undesirable flavors, develop high-quality large yellow croaker liver oil products, in this study, four processes were used to refine the crude oil extraction of large yellow croaker liver (degumming, deacidification, decolorization, distillation and deodorizing) and different deodorization methods (distillation, solid phase adsorption, deodorizing liquid, green tea polyphenols). After treatment, the physical and chemical properties, fatty acids, sensory and volatile flavor components were comprehensively analyzed. The results showed that the acid value of refined large yellow croaker liver oil was reduced by 63.50% compared with crude oil, the iodine value was increased by 1.19 times, and the peroxide value was reduced by 39.02%. The relative content of fatty acids displayed a significant optimization effect. The saturated fatty acids of large yellow croaker liver oil increased by 1.20 times, the polyunsaturated fatty acids increased by 1.71 times, and the content of EPA and DHA increased by 1.84 times. The six deodorization methods can significantly reduce the oily, fishy and sour tastes, and the fatty acid composition and content are slightly different, but the overall quality is relatively high, and their nutritional content and functional characteristics can be guaranteed. The deodorizing effect of GTP is better, it has no effect on the acid value, the iodine value is increased by 1.12 times, the peroxide value is reduced by 37.8%, and the content of unsaturated fatty acids is increased. Among them, the contents of EPA and DHA doubled compared with crude oil. Aldehydes such as 2,4-heptadienal and 2,4-decadienal were reduced by 31.25% and 56.88%, and 3,5-octadienone was removed as much as 81.84%, 1-octene-3 -Alcohol was reduced by 52.76%, and grassy hexanal and nonanal were added, which effectively removed the oily and fishy odor, produced a pleasant fragrance, and greatly improves the flavor of large yellow croaker liver oil. This research provides a theoretical basis for the development of large yellow croaker liver oil and the production technology of liver oil rich in high unsaturated fatty acids and good flavor and high quality.