Residual characteristics and ecological risk of glyphosate in the culture environment of Macrobrachium rosenbergii(M. rosenbergii)were studied. From May to October 2019,the concentrations of glyphosate in aquaculture water,sediment and ridge soil samples of three M. rosenbergii ponds in Jinshan District of Shanghai were monitored,and the ecological risk of glyphosate in aquaculture water and sediment was assessed by the risk quotient method.Results show that the detection rate of glyphosate in aquaculture water was 46.7%,and the maximum residue value was 57 μg/L. The detection rate of glyphosate in culture pond sediment and ridge soil was 83.3% and 77.8%,and the maximum residue value was 1 149 μg/kg and 5 057 μg/kg,respectively. The results of ecological risk assessment showed that the ecological risk level of glyphosate in aquaculture water to the sensitive organisms was mainly no obvious risk and medium risk, while the ecological risk levels in sediment were mainly medium risk and low risk. Research shows that the residual level of glyphosate in the culture environment of M. rosenbergii in Jinshan was at the middle level compared with other areas at home and abroad,and the main source was the use of glyphosate in pond ridge weeding.The residual glyphosate in aquaculture water and sediment had potential risks to M. rosenbergii.