The present study was conducted to investigate the effects of marine animal protein hydrolysates inclusion on growth performance and feed utilization of hybrid grouper, Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂. Seven isonitrogenous and isolipidic diets were formulated with the diet with 60% and 40% fish meal as the positive control (PC) and negative control (NC), respectively. Five diets were formulated with the supplementation of crystal amino acid (CAA), fish soluble (FS), shrimp paste (SP), squid paste (SUP) or the mixture of the above three marine proteins (Mix, 1:1:1). Results showed that the specific growth rate of grouper was significantly reduced in the NC and CAA groups compared to the PC group. However, the supplementation of marine protein hydrolysates significantly neutralized the negative effects on SGR induced by high fishmeal replacement. The fish in the NC group obtained the lowest feed intake (FI), while no significant difference was observed among other treatments. The feed efficiency ratio (FER) and protein efficiency ratio (PER) followed the similar patterns to those of FI. In conclusion, the inclusion of fish soluble, shrimp paste, squid paste and their mixture in low-fish meal diets can significantly improve the growth and feed utilization of hybrid grouper, indicating that the supplementation of marine animal protein hydrolysates can elevate the nutrition value of low fish meal based diets.