Development of EST-SSR marker based on transcriptome and the research of germplasm identification for the new strain (SW-81) of Pyropia haitanensis
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S917

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Major Science and Technology Specific Program of Zhejiang Province (2016C02055-6);The National Key Research and Development Program of China(2018YFD0900606);National Natural Science Foundation of China(31072208);Science and Technology Planning Project of Jiangsu Province, China(BE2018335)

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    Abstract:

    EST-SSR markers were developed based on the transcriptome of Pyropia haitanensis and DNA fingerprinting was constructed using polymorphic SSR markers for the germplasm identification of the new strain SW-81 of Pyropia haitanensis. Experimental results:9 033 microsatellite loci were detected in the transcriptome data, of which the trinucleotide repeat motif was the absolute dominant type, accounting for 95%; Designing primers for microsatellite loci by Primer 3.0 software, and 187 EST-SSR primers were synthesized randomly to analyze the effectiveness, and the 92 pairs of effective primers were finally obtained, of which 42 pairs were polymorphism EST-SSR markers, with the polymorphism amplification rate 22.46%; DNA fingerprints were constructed using the polymorphism EST-SSR marker, which was suitable for 5 strains of P. haitanensis, providing an effective molecular detection method for the germplasm identification of the new strain SW-81. The results above showed that there were abundant microsatellite loci in the genome of P. haitanensis, and the gene sequence of the same loci varied among different strains, showing the polymorphism of amplification products. The polymorphism analysis based on EST-SSR marker can provide an effective means of molecular detection for the management and identification of germplasm resources.

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费勤娇,丁洪昌,张鹏,王铁杆,严兴洪.基于转录组信息的坛紫菜EST-SSR标记的开发及新品系SW-81的种质鉴定[J].上海海洋大学学报,2021,30(3):464-474.
FEI Qinjiao, DING Hongchang, ZHANG Peng, WANG Tiegan, YAN Xinghong. Development of EST-SSR marker based on transcriptome and the research of germplasm identification for the new strain (SW-81) of Pyropia haitanensis[J]. Journal of Shanghai Ocean University,2021,30(3):464-474.

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History
  • Received:May 08,2020
  • Revised:June 26,2020
  • Adopted:July 31,2020
  • Online: May 25,2021
  • Published: May 25,2021
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