Somatostatin (SST), is a kind of neurohormone with key roles in regulating growth and metabolism in all vertebrates. In zebrafish and other fishes, SST peptides are encoded by six genes, and sst1 exists in all vertebrates and is the most evolutionarily conservative. Here, we explored sst functions through a transcriptomic comparison of 6 days post fertilization (dpf) zebrafish sst1^-/- mutants generated by CRISPR/Cas9 gene-editing technology and their wild type counterparts. It is found that several biological processes and biochemical pathways were modified in sst1^-/- zebrafish. The mutant had 354 significantly up-regulated and 504 significantly down-regulated genes. Gene ontologyi(GO) enrichment analysis showed that most of the differentially expressed genes were related to the biosynthesis of amino acids and proteins. Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment indicated metabolic pathways as well as pathways related to amino acid biosynthesis, aminoacyl-tRNA biosynthesis, ribosome biogenesis and rRNA processing were highly represented in the mutant, indicating a more active protein biosynthesis and metabolism. The results of RT-qPCR illustrated that the mRNA expression trends of 10 randomly selected genes were consistent with the results of transcriptome sequencing analysis, indicating that the transcriptome results were accurate and reliable. There was no significant difference in body weight and body length between 6-month-old mutant and wild-type. The results provide a foundation for further exploring the potential functions of the somatostatin gene family.