In order to evaluate the status of different tissues and analyze the functional differentiation of different intestinal segments of black carp under cultivation conditions, histological semi-quantitative system was used to evaluate the gill, liver, spleen and intestine and enzyme activity detection method was used to analyze the distribution of digestive enzyme and antioxidant enzyme in different intestinal segments of black carp. The results showed that the gill, liver, and spleen of black carp were in good condition and with classⅠand class Ⅱ changes and the intestine was the most affected organ and with class Ⅲ changes. Mucosal epithelial cell hyperplasia and lamina propria friable and necrotic were found in Intestinal histological analysis. Fulton’s Conditional factor (CF) of the black carp ranged from 1.01 to 2.30 with an average value of 1.19 and Hepatic somatic index (HSI) ranged from 0.29 to 0.93 with an average of 0.58, which indicated that the detected black carp were in good condition. The distribution of digestive enzyme and antioxidant enzyme in the different intestine segments was significant differences and the activity of amylase, lipase and trypsin in foregut is significantly higher than hindgut. Different from the digestive enzyme, the activity of superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT) and total antioxidant capacity (T-AOC) in the hindgut were significantly higher than foregut. The results can provide references for the evaluation of the tissue status of cultured fish and the analysis of the functional differences between different parts of the intestine.