Macrobenthos were collected in the subtidal waters of the wetland of the north branch of the Yangtze River estuary. The changes of macrobenthos community before and after the release of shellfish in the area were analyzed. A total of 51 species of macrobenthos were collected in the shellfish released area adjacent to the subtidal zone. There are 15 species of mollusks, 17 species of arthropods, 4 species of annelids, 14 species of chordates, and 1 species of nemertea. Eight dominant species were found throughout the year. The annual average density was 0.265 ind/m², the annual average biomass was 0.06 g/m², the annual Shannon-Wiener index was 0.93, the annual Margalef index was 0.895, the Pielou index was 0.39. Compared with the macrobenthic community before release, the release of shellfish affects the composition of benthic communities and dominant species in the adjacent waters of the released area.