The composition and contents of hydrolytic amino acids and fatty acids of greenhouse cultured (40, 60, 74, 94 d) and outdoor cultured (40,60,80,95 d) Litopenaeus vannamei were analyzed during similar growth phases and the nutritional values were assessed. The results showed that:(1)Under the greenhouse culture and outdoor culture mode, the contents of crude protein, crude fat and ash in shrimp muscles showed no obvious difference among Litopenaeus vannamei in similar growth phases.(2)Under the greenhouse culture mode,the unsaturated fatty acids of Litopenaeus vannamei at 40, 60, 74 and 94 d accounted for 64.46%, 60.97%, 62.47% and 63.16% of the total fatty acid content, respectively.Under the outdoor culture mode, the unsaturated fatty acid contents of Litopenaeus vannamei at 40, 60, 80 and 95 d accounted for 67.69%, 64.74%, 65.35% and 65.63% of the total fatty acid content, respectively.The muscles of Litopenaeus vannamei were rich in unsaturated fatty acids under the two cultivation modes, and the polyunsaturated fatty acids of Litopenaeus vannamei in the similar growth stage under the greenhouse cultivation mode were significantly lower than that in the outdoor cultivation mode. (3)The amino acids composition in greenhouse cultured and outdoor cultured Litopenaeus vannamei in similar growth stages were consistent, which contained 17 kinds of amino acids.When Litopenaeus vannamei grew to 40 d, 60 d, 80 d and 95 d in the greenhouse culture mode,ΣEAA/ΣAA ratios were 29.36%, 30.71%, 33.28%, 32.91% and ΣEAA/ΣNEAA ratios were 50.04%, 55.63%, 63.14%, 60.56%.When Litopenaeus vannameigrew to 40 d, 60 d, 80 d and 95 d in the outdoor culture mode,ΣEAA/ΣAA ratios were 34.70%, 33.69%, 32.93%, 34.11%,ΣEAA/ΣNEAA ratios were 64.44%, 63.72%, 62.66%, 66.76%, which basically conformed to the FAO/WHO ideal model. The total amount of amino acids, essential amino acids and delicious amino acids in outdoor cultured Litopenaeus vannamei were higher than those cultured in greenhouse.Conclusions are as follows:Greenhouse cultured and outdoor cultured Litopenaeus vannamei was a kind of shrimp which had high protein, low fat and high nutritional value; The nutritional quality of muscles of outdoor cultured Litopenaeus vannamei was better than that of greenhouse cultured Litopenaeus vannamei.