Red Carassius auratus was used to investigate differences in the extraction efficiency of carotenoids by different extraction processes. The optimum extraction conditions including different factors were determined by single factor test and orthogonal test respectively. According to the results, the best extraction conditions of carotenoids extraction process of red Carassius auratus are showed as follows:dried red Carassius auratus was used as the sample, the ratio of solid to liquid is 1:15, the extraction was conducted at 80℃, for 90 min each time, twice, organic solution ratio (ethanol to acetone) is 1:2. The carotenoid extracted can reach up to 140.58 mg/kg. An important basis is provided for the development on the extraction process afterwards and some references are also provided for the further study on the determination of carotenoids in goldfish.