Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish (Takifugu oblongus)

doi:10.12024/jsou.20180302233

Home > Archive>Volume 27, Issue 6, 2018 >924-929. DOI:10.12024/jsou.20180302233

Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish (Takifugu oblongus)
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                        10.12024/jsou.20180302233
                    
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Affiliation:College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road,无,College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road,College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road,College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road,College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road,College of Food Science and Technology,Shanghai Engineering Research Center of Aquatic-Product Processing Preservation,Shanghai Ocean University,No ,Huchenghuan Road
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Abstract:

To provide some advice for people when they eat pufferfish, the toxicity of wild pufferfish(Takifugu oblongus Bloch) were investigated and compared with the toxicity of artificial feeding pufferfish (Takifugu flavidus; Takifugu obscurus). In this study, 17 pufferfish tissues were randomly selected, next the tissue was extracted by boiling in 2% acetic acid. After centrifugation, the protein of supernatant was removed by ultrafiltration. The toxicity of 17 wild Takifugu oblongus samples was detected by magnetic immunochromatographic test strip prepared by our laboratory. The representative samples were analyzed and compared with mouse bioassay in regard to the toxicity. Our results showed that the toxicity of muscle and testicle was hypotoxicity, ranging between 0-7 μg/g because of individual difference; The toxicity of ovary and liver were in the range of 4-400 μg/g, was strongest toxicity; The tissues of cultured Fugu flavidus and Fugu obscures were non-toxic.

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梁田田,卢玉平,潘迎捷,方国锋,林昕,黄天娇,卢瑛.免疫磁珠层析试纸法快速检测野生横纹东方鲀毒性[J].上海海洋大学学报,2018,27(6):924-929.
LIANG Tiantian, LU Yuping, PAN Yingjie, FANG Guofeng, LIN Xin, HUANG Tianjiao, LU Ying. Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish (Takifugu oblongus)[J]. Journal of Shanghai Ocean University,2018,27(6):924-929.

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Received:March 07,2018
Revised:April 30,2018
Adopted:June 06,2018
Online: December 02,2018
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