Consumers' demand for freshness and safety of live aquatic products has become stricter and stricter in recent years, and live products transportation has become an effective way of keeping products fresh. However, there are some problems about domestic live products transport technology, such as the low degree of informative and intelligence control plus single monitoring factor, which are all urged to be solved. In view of the above, one intelligent monitoring system was designed for live aquatic products transportation. By the design of hardware and software, it realized the real-time monitoring of water temperature, pH, dissolved oxygen and other water quality parameters as well as the video data storage in the transport carriage. This system constructs three levels of Internet of Things (IoT), which are bottom sensor detection and control, center data controlling and processing, remote terminal management. It realized the remote intelligent monitoring of live aquatic products transportation by using wireless transmission technology. In addition, the system has designed an host monitoring interface by using LabVIEW, it is not only able to monitor the real-time water quality parameters and video data storage in the transport carriage, but also established a database of aquatic products which can be used to inquire the historical data. The system has been tested by small transport vehicles for live tilapia, which proved that the system is easy to operate and stable, reliable and practicable.