Comprehensive studies on triploid induction technology will greatly improve the development of Takifugu bimaculatus industry. This study applied hydrostatic pressure method to induce T. bimaculatus triploidy and investigated optimal inducing conditions of the starting time, the pressure and the treatment duration. Results showed that 96.67% triploid rate and 77% hatching rate were achieved under such condition:treatment temperature as 18-20℃, starting at 5 min after fertilization, hydrostatic pressure as 10-15 Mpa and treatment duration as 5-7 min. This is the first report for T. bimaculatus triploidy by hydrostatic pressure,which may provide an efficient technological process for T. bimaculatus triploidy. It also can offer a good reference for triploid induction in other Takifugu species.