To optimize the formulation and preparation technology of Fungi Fibrinolytic Compound 1 (FGFC1) powder injection by experiments, the appearance, drug content, water content, redissolving time and pH of the freeze-dried FGFC1 powder were investigated to determine the best formulation and preparation technology. When the formulation craft is FGFC1: Sodium citrate: mannitol = 1:3:10, -48 ℃ freeze-dried for 48 h, the appearance of the powder injection is well, the average redissolving time is (15.33±1.53) s, pH =6.86±0.03, concentration of FGFC1 has no obvious change in glucose injection and mannitol injection. This freeze-dried power is stable in high humidity conditions, and has a certain degree of degradation at high temperature and strong light environment, and drug properties are very stable under low temperature conditions. The FGFC1 freeze-dried power formulation is reasonable, and the preparation technology is feasible.