Molecular mechanism and the cause for high ratio of male Pengze crucian carp under high-density culture condition

doi:10.12024/jsou.20150101299

Home > Archive>Volume 24, Issue 6, 2015 >826-833. DOI:10.12024/jsou.20150101299

Molecular mechanism and the cause for high ratio of male Pengze crucian carp under high-density culture condition
DOI:
                        10.12024/jsou.20150101299
                    
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                        ZHENG YaoZHENG Yao
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China;Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture;Wuxi 214081, Jiangsu, China;Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
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QU JianhongQU Jianhong
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China;Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture;Wuxi 214081, Jiangsu, China;Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
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BING XuwenBING Xuwen
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China;Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture;Wuxi 214081, Jiangsu, China;Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
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CHEN JiazhangCHEN Jiazhang
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China;Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture;Wuxi 214081, Jiangsu, China;Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
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WANG ZaizhaoWANG Zaizhao
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China;Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, Shaanxi, China
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Affiliation:Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,College of Animal Science and Technology, Northwest A&F University
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Abstract:

In laboratory culture conditions, a high proportion of gynogenic Pengze crucian carp (Carassius auratus var. Pengze, Pcc) male fish occurred in F₁ progenies compared with pond culture conditions. All these F₁ progenies were bred from the same parents and obtained via artificial gynogenic method. In order to find the accurate relationship between this variant sex ratio and the culture density, gynogenic PccF₂ progenies were obtained from female PccF₁ offspring and activated by the sperm of red carp (Cyprinus carpio var. red style). They were maintained in different culture densities. Then we chose the male ones from the high-density culture groups, and the female ones from the low-density culture groups. Ovarian gene expression profiles were detected and compared between the male and female F₂ progenies respectively. Results showed that ovarian transcripts of dmrt1c, anti-Mullerian hormone (amh), aromatase (cyp19a1a) were higher than those corresponding transcripts in the testis of PccF₂ offspring both in low-density and high-density culture groups. Ovarian dmrt1a, dmrt1b, 3β-hydroxysteroid dehydrogenase (3bhsd), 11β-hydroxysteroid dehydrogenase 2(11bhsd2), 17α-hydroxylase/17,20-lyase 1(cyp17a1) and steroidogenic acute regulator protein (star) were lower than those corresponding transcripts in the testis of PccF₂ offspring both in low-density and high-density culture groups. The gene expression profiles for steroidogenic genes of PccF₂ progenies differed from different genders and densities in laboratory culture conditions, which may cause the phenomenon of high proportion of the existing male ones.

Key words:culture density;sex differentiation related genes;estrogen and androgen steroid receptor;steroidogenic enzyme;gene

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郑尧,瞿建宏,邴旭文,陈家长,王在照.高密度养殖彭泽鲫造成雄鱼较多的分子机制及成因分析[J].上海海洋大学学报,2015,24(6):826-833.
ZHENG Yao, QU Jianhong, BING Xuwen, CHEN Jiazhang, WANG Zaizhao. Molecular mechanism and the cause for high ratio of male Pengze crucian carp under high-density culture condition[J]. Journal of Shanghai Ocean University,2015,24(6):826-833.

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Received:January 08,2015
Revised:July 23,2015
Adopted:September 09,2015
Online: December 01,2015
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