The effects of the different dietary carbohydrates and different carbohydrate levels on GH /IGF-Ⅰ gene expression, body composition and amino acid composition were studied in juvenile mirror carp (Cyprinus carpio). 420 Songpu mirror carp (mean weight=8.30±0.15g) were randomly divided into 4 dietary treatments triplicate groups and 35 fish per triplicate. The fish in 4 groups were fed two carbohydrates (starch and glucose) diets with two levels (250 g/kg and 500 g/kg) (LS/HS/LG/HG) and were reared for 60 days. Results showed that the GH mRNA expression levels of HS and LS groups were significantly lower than that of LG group (P<0.05). The IGF-Ⅰ mRNA expression level of HG group was significantly lower than that of LS and LG groups (P<0.05). The crude protein was not significantly different among groups (P>0.05). The moisture of HS group was significantly lower than that of the other groups (P>0.05). The crude fat of HS group was significantly higher than that of the other groups (P>0.05). The body amino acid composition results showed that Leu and Cys contents of HS group were significantly lower than those of LG group (P<0.05). Ala content of HS group was significantly lower than that of LS and LG groups (P<0.05). The other amino acids contents were not significantly different among groups (P>0.05). The total essential amino acids, semi-essential amino acid, total non-essential amino acids and total amino acids had no significant difference mong groups (P>0.05). In conclusion, high dietary starch could inhibit the expression of GH and IGF-Ⅰ genes, increase body crude fat content and decrease some amino acids contents.