Isolation and identification of an efficient aerobic denitrifier
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Fisheries Institute of Zhoushan,Zhoushan Fisheries Research Institute of Zhejiang Province, Aquaculture Research and Promotion Center, Zhejiang Marine Development Research Insitute Zhoushan 316000, China,Zhoushan Fisheries Research Institute of Zhejiang Province,Food and Drug Testing Institute of Zhoushan,Food and Drug Testing Institute of Zhoushan,Fisheries Technology Extention Station of Zhoushan,Food and Drug Testing Institute of Zhoushan,Food and Drug Testing Institute of Zhoushan

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    Abstract:

    Isolation and identification of an efficient aerobic denitrifying bacteria strain by using the selective medium to isolate and screen aerobic denitrifying bacteria through the quantitative increase of nitrite content, after a preliminary screening, and then second screening through denitrification performance testing for bacterial isolation. Physiological and biochemical tests and 16S rRNA gene amplification and blasting in GenBank ,and phylogenetic tree construction were used to identify the selected strain. Only the DB-6 with effective denitrification .During 5 days, NO2- -N was decreased from 3 570 mg/L to 22 mg/L, the removal efficiency was 99.4%, NO3- -N was decreased from 2 464 mg/L to 27 mg/L, the removal efficiency was 98.9% and without nitrite accumulation. The strain was identified as Enterobacter cloacae DB-6 according to its morphological, physiological and biochemical characters. In order to further confirm the result, 1 445 bp sequence of DB-6 16S rRNA was amplified and blasted in GenBank. Homology analysis study showed that DB-6 has the highest similarity to Enterobacter cloacae with 99% identity. And phylogenetic tree was constructed together with the discovered nitrifying bacteria, denitrifying bacteria and Enterobacter cloacae. It was suggested that Enterobacter cloacae DB-6 be a novel strain of aerobic denitrifying bacteria,which will be helpful in nitrogen removal of mariculture water.

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彭志兰,柳敏海,郭海波,罗海忠,薛超波,吴益春,罗海军,夏枫峰.一株高效好氧反硝化细菌的分离与鉴定[J].上海海洋大学学报,2015,24(4):632-639.
PENG Zhilan, LIU Minhai, GUO Haibo, LUO Haizhong, XUE Chaobo, WU Yichun, LUO Haijun, XIA Fengfeng. Isolation and identification of an efficient aerobic denitrifier[J]. Journal of Shanghai Ocean University,2015,24(4):632-639.

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History
  • Received:October 22,2014
  • Revised:March 20,2015
  • Adopted:March 30,2015
  • Online: July 22,2015
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