Effects of starvation on immune function of hemolymph in Pinctada martensii during pre-operation were investigated under laboratory conditions, including total hemocyte counts (THC), superoxide dismutase (SOD), catalase (CAT), lysozyme (LSZ), acid phosphatase (ACP) and alkaline phosphatase (AKP). There were three treatments in the experiments: feeding once daily, feeding once two days, and feeding twice daily as a control. Hemolymph was sampled at the beginning of the experiment, and 1, 2, 3, 4 weeks to evaluate the immune functions. The results showed THC, SOD, CAT, LSZ, ACP and AKP activities decreased with the increase of fasting degree after one week fasting, immune parameters of the two fasting treatments significantly reduced compared with the control group, and displayed significant difference (P<0.05). All immune parameters in feeding once two days treatment were lower than those of the control treatment, and the trend lasted for four weeks. Therefore, starvation can significantly reduce the physiological functions and immune functions, and this paper can provide theoretical basis for the pre-operation of pearl culture.