Based on the terminal inverted repeats of Tc1-like superfamily transposons from fish, a single primer was designed to amplify similar transposon in genome of Salmo trutta fario Linnaeus. Two Tc1-like transposons sequences were amplified. The sequencing results showed that the length of the obtained fragments were 1 607 bp and 1 473 bp, named Tbt1 and Tbt2. Sequence analysis showed that there was a 196 nt and a 225 nt inverted terminal repeats (ITR) at the ends of Salmo trutta fario Tbt1, respectively. Two 12 nt Sub-terminal inverted repeats (SIR) were found among the left and right ITR. In contrast, Salmo trutta fario Tbt2 transposon had a 32 nt and 31 nt short ITRs, and the left and right ITRs each contained only one 12 nt of SIR. During evolutionary process, 4 and 9 stop codon mutations had been accumulated among transposase coding region in Salmo trutta fario Tbt1 and Tbt2, respectively, which led both transposons to lose their capacity to produce transposases. The Salmo trutta fario Tbt2 had a sequence similarity less than 30% to other salmon Tc1-like transposons, while there had a sequence identity as high as 98% to goldfish Tca2 transposon. This indicates that this transposon is very likely originated in the horizontal gene transfer mode.