• Article
  • | |
  • Metrics
  • | |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    A method to determine tetrodotoxin (TTX) in globefish by solid phase extractions and gas chromatography mass spectrometry (GC-MS) is described. TTX in globefish sample was extracted with 2% acetic acid/methanol, defatted by petroleum ether, evaporated to dryness under reduced pressure. Then the compound was converted to 2 amino 6 hydroxymethyl 8 hydroxyquinazoline (C9 base) under alkaline conditions and extracted by use of an MCX extraction cartridge. The C9 base was analyzed after trimethylsilyl derivation (TMS) C9 base by GC-MS scan mode, the qualified ions are 376, 392, 407. (TMS) C9 base is quantified by GC-MS selected ion monitoring. The quantified ion was 392. The calibration curve was linear in the range of 0.05-5.0 μg /mL and linear relationship R2=0.997 5. For the 0.02,0.1,0.5 mg/kg spiked samples , the recovery rate were 64.0%-92.8%, RSDs were 5.41%-8.63% for six times, and the limitation of quantification was 5.0 μg/kg. From the results of above, detection of TTX in aquatic products by solid phase extractions and gas chromatography/mass spectrometry (GC MS) is sensitive, accurate and feasible.

    Reference
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

黄清发,孙振中,戚隽渊,孙建华,黄德凤,张玉平,刘 琴.河鲀毒素固相萃取-气相色谱-质谱法研究[J].上海海洋大学学报,2012,21(6):1058-1063.
HUANG Qing-fa, SUN Zheng-zhong, QI Jun-yuan, SUN Jian-hua, HUANG De-feng, ZHANG Yu-ping, LIU Qin. A method to determine tetrodotoxin (TTX) in globefish by solid phase extractions and gas chromatography mass spectrometry (GC-MS)[J]. Journal of Shanghai Ocean University,2012,21(6):1058-1063.

Copy
Share
Article Metrics
  • Abstract:3364
  • PDF: 2731
  • HTML: 0
  • Cited by: 0
History
  • Online: November 22,2012
Article QR Code