Screening and identification of a cellulase producing marine bacterium and optimization of its fermentation conditions
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    Abstract:

    A total of 23 cellulase producing marine bacteria had been isolated from Ningbo Sea area by the CMC/Congo Red method. Then the alkaline cellulase producing strain MB117 had been made an intensive study of its production of cellulase. According to the 16S rDNA analysis, the strain belonged to the Pseudoalteromonas sp.. The optimum initial pH of culture medium for the cullulase production was 7.0. And with 1% inoculum and 10% broth content, the maximum production of cellulase can be obtained. Low temperature is good for the growth and cullulase producing of MB117. The optimum pH for cellulase activity was 9.0, and the strain has a high enzyme activity and a good stability in the basic conditions. The optimum temperature for cellulase activity was 40 ℃.

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曲均革,姚晓敏,朱 鹏,严小军.产纤维素酶海洋细菌的筛选鉴定和产酶条件优化[J].上海海洋大学学报,2012,21(6):1053-1057.
QU Jun-ge, YAO Xiao-min, ZHU Peng, YAN Xiao-jun. Screening and identification of a cellulase producing marine bacterium and optimization of its fermentation conditions[J]. Journal of Shanghai Ocean University,2012,21(6):1053-1057.

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  • Online: November 22,2012
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