• Article
  • | |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • | |
  • Comments
    Abstract:

    According to zebrafish GDF9 and BMP15 gene conservative region of nucleotide sequence to design primers, the GDF9 and BMP15 gene fragment of Carassius curatus cuvieri was amplified by RT PCR. The partial sequence of GDF9 is 778 bp and codes a protein of about 145 amino acids, the partial sequence of BMP15 is 811 bp and codes a protein of about 270 amino acids. The amino acid sequence comparison results showed that the GDF9 gene of Carassius curatus cuvieri shared 58%-78% identity with other species,and Carassius curatus cuvieri is most similar to zebrafish(78%); BMP15 gene ofCarassius curatus cuvieri shared 30%-80% identity with other species,and it is most similar to zebrafish(80%).Phylogenetic tree of GDF9 and BMP15 gene generated by Neighbor Joining method suggested that all teleosts cluster together,and Carassius curatus cuvieri GDF9 is clustered closely with that of zebrafish, rainbow trout and European sea bass, and Carassius curatus cuvieri BMP15 is clustered closely with that of zebrafish and European sea bass.Then the expressions of different tissues including spleen, gill, liver, brain, heart, muscle, kidney and ovary were analyzed by semi quantitative PCR.The results showed that the BMP15 gene of Carassius curatus cuvieri was expressed in all tissues and highest in ovary; the GDF9 gene of Carassius curatus cuvieri was expressed in liver, brain, heart, muscle and ovary, while highest in ovary and extremely low even no expression in spleen, gill and kidney. This study will be helpful for further understanding the structure and function of GDF9 and BMP15 gene.

    Reference
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

王 瑶,陈阿琴,杨志刚,刘志伟,郭子好,曾奇韬,沈 城.日本白鲫BMP15GDF9基因片段的克隆及组织表达分析[J].上海海洋大学学报,2012,21(5):693-700.
WANG Yao, CHEN A-qin, YANG Zhi-gang, LIU Zhi-wei, GUO Zi-hao, ZENG Qi-tao, SHEN Cheng. Cloning and tissue expression of GDF9 and BMP15 genes in Carassius auratus cuvieri[J]. Journal of Shanghai Ocean University,2012,21(5):693-700.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Online: October 12,2012
Article QR Code