The optimum conditions for the extraction of cadmium from Oyster homogenate by succinic acid and citric acid were determined using single facter and orthogonal array design methodology. The results showed that the pH value of organic acid has the maximal effect on extraction rate of cadmium in Oyster homogenate, followed by materialliquid ratio and extraction time. The effects of concentration and temperature on extraction rate were found to be slightly dependent on the type of organic acid. The optimal conditions to obtain the highest extraction rate by succinic acid and citric acid were 2 h, 35 ℃, pH 3.0, 0.02 mol/L of sucinic acid and 1∶15 of material to solution, as well as 2 h, 35 ℃, pH 2.5, 0.04 mol/L of citric acid and 1∶15 of material to liquid, respectively. Under these optimal conditions, extraction rate of cadmium from Oyster homogenate by the two organic acids reached up to 90.2%-91.8%.