To discuss the relationship between fatty acid and the ovary development, the lipid contents and fatty acid compositions of muscle, liver and egg were analysed using gas chromatography. Comparisons were made between Ⅲ-Ⅳ and Ⅴ stages of ovary development in wild broodstocks of Paralichthys olivaceus. The results indicated that there were significant differences of lipid contents among muscle, liver and egg tissues. The lipid content in liver of Ⅲ-Ⅳ stages was significantly lower than that in Ⅴ stage. There were significant differences of fatty acid compositions in muscle, liver and egg tissues between Ⅲ-Ⅳ andⅤ stages, especially PUFA.There were higher relative contents of n3HUFA, DHA, DPA, EPA in egg at Ⅴ stage than those in liver and muscle at the same stage. The ratio of DHA/EPA in liver of Ⅲ-Ⅳ stage broodstocks was significantly higher than those at Ⅴ stage, but the ratio of EPA/ARA was significantly lower. These results suggested that some fatty acids were essential to Paralichthys olivaceus during sexual maturation. In order to ensure the normal reproduction, during cultivating, PUFA, especially DHA, EPA and ARA need to be supplemented.