The total RNA was extracted from brain of zebra fish.The GnRH cDNA was amplified by RT-PCR method using isolated total RNA as template.The GnRH cDNA is 646 bp in length that contain an open reading fram(ORF) of 258 bp,which encode the 86 amino acids residues that including a signal peptide(SP),a GnRH decapeptide and a GnRH-associated peptide(GAP) which is linked by the processing site Gly-Lys-Arg sequence.The cGnRH cDNA encode SP of 24 amino acids residues and GAP of 49 amino acid residues.The sequence stru...