Genetic diversity and phylogenetic relationship in goldfish(Carssius auratus var) were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp.The eight representative varieties were Red Common Goldfish(RC),Red Fantail(RF),White Oranda with Red Carp(WR),White Pearlscale(WP),Red Tigerhead(RT),Black Moor(BM),Red Moor with Butterfly Tail(MB) and Red Bubble-eye with Dorsal Fin(RB).699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism.Sequences data showed that all 129 sequences were grouped into 17 haplotypes.Nine representative varieties shared same haplotype.Five representative varieties of goldfishes(BM,MB,WR,RF,RC)had haplotypes respectively.The results from the analyses of genetic distance and cluster analysis showed that six representative varieties(WP,BM,MB,RT,WR,RB) were clustered together first,which then clustered together with RF and finally clustered together with RC and WC.Wild common carp evolved to common goldfish first,and then common goldfish evolved to some primitive varieties of fantail goldfish,then differentiated to other representative varieties.