Litopenaeus vannamei(body weight of 0.084g)were fed on basal diet with 0.0%,0.1%,0.5%,1.0%,1.5%,2.0%,2.5% of the peptides for 37 days respectively, with basal diet as the control. Activities of protease and amylase of hepatopancreas, stomach and the intestine of Litopenaeus vannamei were measured. The result showed that the peptides had no significant effect on the activities of tryptase in the hepatopancreas(P>0.05). Activities of pepsin of the stomach and the hepatopancreas and activities of tryptase and amylase of the stomach and the intestine in 1.0% group and 1.5% group were significantly higher than that of the control(P>0.01). The activity of amylaes of the hepatopancreas in 1.5% group was notably significantly higher that of the control(P>(0.05)).