The different desalination methods and salinities had an effect on survival rate and growth of Penaeus Vannamei juvenile. The best result, 95.0% of survival rate, was obtained by siphoning , intaking while flowing out. The desalination method feasible for the large scale of production was desalinating once every 6 hours and the rate of reduced salinity was 0.76/h. This salinity method got 83.3% of the survival rate. The different size of juvenile (body length: 0.59-2.22cm, weight: 8.12-30.12mg) had the same ability to tolerate the reduced salinities. The suitable range of salinities for the growth of Penaeus vannamei juvenile was 4.9-36.3, the most optimum 7.5-24.6,the juvenile could adapt to 0.59-36.3 of salinity range. Penaeus vannamei juvenile can grow in freshwater (S:0.59,0.62) where after 21d the increase rate of body length was 67.5%-73.0%, the increase rate 599.5%-615.6% for body weight, and survived rate 88.4%-90.0% larvae.