大陈岛海域野生大黄鱼子代遗传多样性的初步对比分析
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S932.4

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国家重点研发计划(2023YFD2401903,2019YFD0901303)


A preliminary comparative analysis of genetic diversity in Larimichthys crocea progeny from Dachen Island marine waters
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    摘要:

    为更深入了解大黄鱼资源的恢复状况,基于2020—2021年大陈岛局地尺度4个季度的拖网采样数据,通过捕获的野生大黄鱼(Larimichthys crocea)幼鱼的体长参数与增殖放流个体的比对,初步判别其是否属于野生繁殖群体,测定大陈岛海域获取的大黄鱼的野生成鱼样本(LCA)、野生幼鱼样本(LCJ)以及铜网养殖群体样本(LCC)线粒体COⅠ基因序列片段差异,以初步探讨增殖放流对大黄鱼野生繁殖群体的遗传结构和多样性的影响。结果显示:研究海域采集的大黄鱼幼鱼集中出现在春季,其体长范围为11.24~70.00 mm,平均体长(33.46±12.90) mm,显著低于增殖放流群体的最小体长规格标准(>5 cm)。野生大黄鱼成鱼及稚鱼具有较高的单倍型多样性和中等的核苷酸多样性(Hd=0.516~0.879,Pi=0.001 8~0.003 7)。遗传分化指数显示,3个群体之间遗传分化程度极小,群体间基因流为6.814 1~∞,无显著遗传结构差异。系统发育和网络分析表明,野生群体与养殖群体间具有较高的遗传相关性。研究表明:增殖放流或网箱逃逸所形成的大黄鱼野外种群已经在大陈海域繁殖产生后代,并已形成一定规模的补充群体,且呈现基因突变的累积效应,大陈海域大黄鱼资源的恢复条件已逐渐成型。

    Abstract:

    To gain a deeper understanding of the recovery status of Larimichthys crocea (large yellow croaker) resources, this study analyzed four seasons of trawl survey data collected from the Dachen Island region at a local scale during 2020-2021. By comparing the body length parameters of captured wild juvenile L. crocea with those of stock-enhanced individuals, we preliminarily identified whether they belonged to wild reproductive populations. Mitochondrial COⅠ gene sequences were determined for wild adult samples (LCA), wild juvenile samples (LCJ), and copper-net-cultured samples (LCC) from the Dachen Island waters to preliminarily assess the impact of stock enhancement on the genetic structure and diversity of wild L. crocea populations.The results showed that wild juvenile L. crocea in the study area were predominantly found in spring, with body lengths ranging from 11.24 to 70.00 mm (mean: 33.46 ±12.9 mm), significantly smaller than the minimum size standard (>5 cm) of stock-enhanced individuals. Wild adults and juveniles exhibited high haplotype diversity and moderate nucleotide diversity (Hd = 0.516-0.879, Pi = 0.001 8-0.003 7). Genetic differentiation indices revealed minimal divergence among the three populations, with gene flow values ranging from 6.8141 to ∞, indicating no significant genetic structure differences. Phylogenetic and network analyses demonstrated high genetic relatedness between wild and cultured populations. This study suggests that the wild L. crocea population in the Dachen Island waters likely originated from stock enhancement or escapees from net pens, which have successfully reproduced and formed a replenishment population of observable scale. Moreover, these populations exhibit an accumulation of genetic mutations, indicating that the conditions for the recovery of L. crocea resources in the Dachen Island waters are gradually being established.

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蔡鹏雨,邹沁东,汪振华,章守宇.大陈岛海域野生大黄鱼子代遗传多样性的初步对比分析[J].上海海洋大学学报,2025,34(6):1358-1367.
CAI Pengyu, ZOU QinDong, WANG Zhenhua, ZHANG Shouyu. A preliminary comparative analysis of genetic diversity in Larimichthys crocea progeny from Dachen Island marine waters[J]. Journal of Shanghai Ocean University,2025,34(6):1358-1367.

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  • 收稿日期:2025-03-31
  • 最后修改日期:2025-05-20
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  • 在线发布日期: 2025-12-06
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