To investigate the effects of light color on the behavior and physiology of juvenile Sebastes schlegelii， the behavioral responses of individuals and populations of Sebastes schlegeliiwere studied under five different light colors. Five color light zones， including white， red， yellow， green， and blue， were set up in the experimental water tank to record the behavioral responses of individuals and populations.At the same time， samples were taken and analyzed for plasma cortisol （COR） concentration， glucose （GLU） content， aspartate aminotransferase （AST） and alanine aminotransferase （ALT） activity at 2， 4， 8， and 12 hours after the start of the experiment.The results showed that Sebastes schlegelii had a certain preference for light color. The proportion of residence time of individual Sebastes schlegelii under different light colors was blue light 22.08%， green light 20.55%， white light 17.59%， yellow light 15.04%， and red light 15.03%， respectively. Blue light was significantly higher than yellow and red light， and there was no significant difference between green light， white light， yellow light， and red light. The aggregation rate of Sebastes schlegeliiin the group was blue light 57.33%， yellow light 15.05%， white light 13.10%， green light 8.49%， and red light 3.89%， respectively. Blue light was significantly higher than the other four light colors， while red light was significantly lower than yellow and white light， and there was no significant difference compared to green light.Both individuals and groups showed a preference （positive trend） for blue light and an avoidance （negative trend） for red light， while their preference for yellow， white， and green light was not significant.There was a significant difference in plasma cortisol concentration among the 5 different light color conditions， with blue light being the lowest， followed by yellow light， white light， and green light， and red light being the highest. There was no significant difference in glucose content in plasma under blue， yellow， and green light conditions， while glucose content was higher under red light conditions. The effects of the five light colors on AST and ALT activity in plasma were not significant.Research showed that the color of light had a certain impact on the behavior and physiology of Sebastes schlegelii， and there was a significant negative correlation between preference selection behavior and stress physiology and metabolic levels. The Sebastes schlegeliiexhibited a preference for blue light in behavior， lower plasma cortisol and glucose levels in physiology， avoidance of red light， and higher plasma cortisol and glucose levels in physiology.