The intermuscular bones of fish are closely related to their locomotion； the more athletic the fish is, the greater the number of intermuscular bones and the relatively higher the muscle fat content. In order to investigate whether C. brachygnathus exists migratory ecological taxa in Poyang Lake, samples of C. brachygnathus were collected from different sampling sites of Poyang Lake, such as Qianyanqiao. In addition, samples of C. nasus from Zhoushan Shengsi and Qingcaosha Reservoir at the mouth of the Yangtze River were collected in order to investigate the effect of migration on the formation of intermuscular bones of C. nasus. The morphology, number and muscle protein and fat content of medullary arch ossicles and pulse arch ossicles were compared by anatomical method and conventional measurement method. The results showed that： (1) The number of intermuscular bones of C. brachygnathus was the least in the Poyang Lake which was 100.67±6.34； The number of intermuscular bones in Qianyanqiao was the largest which was 127.67±6.18. (2)The intermuscular bone number of C.nasus in Poyang Lake was 129.60±0.98 which was significantly higher than C. brachygnathus of 110.60±2.97. (3) The number of Poyanghu intermuscular bones of the same species was significantly higher than that of 113.67±3.29 intermuscular bones of the Qingcaosha but lower than that of 147.67±3.09 intermuscular bones of Shengsi ； (4) The protein content of muscle in the C.nasus in Poyang Lake was lower than that of the C. brachygnathus, and the fat content was higher than that of the C.brachygnathus. Suggesting that the C.nasus need to oscillate more in order to fulfil the needs of long-distance migration and to stimulate more intermuscular spine formation. These results are important for the understanding of the difference in the origin of the migratory populations of C. nasus and C. brachygnathus in Poyang Lake, and lay the foundation for the further research on the biology of the migratory populations in Poyang Lake.