In order to understand the feeding habits of Ommastrephes bartramii and explore its position in the food web and ecological function, we analyzed the food composition of 15 Ommastrephes bartramii samples which were collected in the high sea of Northwest Pacific Ocean using high-throughput sequencing.The results showed that there were 646 980 high-quality sequences in 15 samples, a total of 624 operational taxonomic units （OTUs） were obtained through cluster analysis. A total of 65 food species, belonging to 6 phyla, 11 classes, 22 orders, 37 families and 51 genera, were detected by screening and comparison.Ommastrephes bartramii fed mainly on cephalopods, fishes, and a variety of zooplanktons （including Euphausiid, Ostracod, Medusae, etc.）. The dominant prey species included Sardinops sagax, Onychoteuthis borealijaponica, Gonatopsis borealis, Watasenia scintillans, Notoscopelus caudispinosus, etc. In conclusion, the feeding range of Ommastrephes bartramii was wide, and the food species were related to the natural preys in the sea area. Furthermore, Ommastrephes bartramii showed different feeding preferences among different mantle length groups. The analysis of the whole composition of stomach contents of Ommastrephes bartramii plays an important role in digging out the relationship between feeding and life history, such as growth, migration and reproduction. This provides a scientific basis for the sustainable development and conservation of Ommastrephes bartramii resources from the perspective of ecosystem.