水体中添加乳酸钙对中华绒螯蟹生长、蜕壳、钙沉积和水质指标的影响
CSTR:
作者:
中图分类号:

S966.16

基金项目:

财政部和农业农村部国家产业技术体系建设项目(CARS-48)


Effects of adding calcium lactate to water on growth, molting, calcium deposition of Chinese mitten crab and water quality
Author:
  • HUANG Junjie

    HUANG Junjie

    Shanghai Aquaculture Engineering and Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Rice-Fishery, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHU Shaicheng

    ZHU Shaicheng

    Shanghai Aquaculture Engineering and Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Rice-Fishery, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • MA Tianli

    MA Tianli

    Shanghai Aquaculture Engineering and Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Rice-Fishery, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • JIANG Dongliang

    JIANG Dongliang

    Henan Jindan Lactic Acid Technology Co., Ltd, Dancheng 477150, Henan, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • CHENG Yongxu

    CHENG Yongxu

    Shanghai Aquaculture Engineering and Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Rice-Fishery, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [40]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为研究水体中添加乳酸钙对中华绒螯蟹幼蟹生长、蜕壳及体内钙沉积的影响,本研究分别在淡水中添加0、218、436和654 mg/L的乳酸钙,使水体中含有45.6、84.4、123.2和163.6 mg/L的钙离子(分别记为1# ~ 4#组),对初始体质量为(4.78±0.51) g的中华绒螯蟹雌性幼蟹进行为期90 d(经历2个完整蜕壳周期)的养殖实验。结果表明:(1)随着水体乳酸钙浓度的增加,幼蟹的成活率显著上升;(2)随水体乳酸钙含量增加,第一次蜕壳后体质量、体质量增长率、特定生长率和肝胰腺指数均呈上升趋势,但差异不显著;而第二次蜕壳后幼蟹体质量、壳长和壳宽增长率均随着水体乳酸钙添加显著升高,其中3#组和4#组显著高于1#组;(3)添加乳酸钙对蜕壳间隔无显著差异,但可显著降低幼蟹的残肢率;(4)在钙沉积方面,添加乳酸钙可以显著提高幼蟹第一次蜕壳和第二次蜕壳阶段中血淋巴钙含量以及第一次蜕壳阶段幼蟹肌肉中的钙含量;(5)乳酸钙含量的增加可显著提高es EcR2es RXR基因的表达丰度;(6)添加乳酸钙对水体pH、氨氮和亚硝酸盐无显著影响,而硬度和碱度随着乳酸钙含量增加而显著上升。综上,水体添加654 mg/L乳酸钙可提高幼蟹成活率、生长性能、组织中钙沉积和蜕壳相关基因表达丰度,并能降低幼蟹蜕壳后的残肢率。

    Abstract:

    In order to study the effects of calcium lactate to water on the growth, molt, and calcium deposition of juvenile Chinese mitten crab (Eriocheir sinensis), this study conducted a 90-day (two complete molting cycles) rearing experiment on female juvenile crabs with an initial body mass of (4.78±0.51) g. Calcium lactate was added to freshwater at concentrations of 0 mg/L, 218 mg/L, 436 mg/L and 654 mg/L and the calcium ion content in water is 45.6 mg/L, 84.4 mg/L, 123.2 mg/L and 163.6 mg/L.(recorded as Treatment 1# to 4#). The results showed: (1) The addition of calcium lactate improved survival rates of juvenile E. sinensis significantly. (2) With the increase in calcium lactate content, there was an upward trend of body mass, weight gain rate, specific growth rate, and hepatopancreas index of juvenile E. sinensis during the first molting cycle, but the differences were not significant. However, during the second molting cycle, the body weight, carapace length and carapace width growth rates of juvenile E. sinensis significantly increased with the calcium lactate addition, and among them, treatment 3# and 4# were significantly higher than treatment 1#. (3) The addition of calcium lactate had no significant effect on the intermolt duration but significantly reduced the rate of limb loss after each molting. (4) In terms of calcium deposition, the addition of calcium lactate significantly increased the calcium content in hemolymph during the first and second molting cycles, as well as calcium content in the muscle during the first molting cycle. (5) Increasing calcium lactate content significantly elevated the expression of es EcR2- mRNA and es RXR- mRNA. (6) Calcium lactate had no significant effect on water pH, ammonia nitrogen and nitrite, but water hardness and alkalinity significantly rose with increasing calcium lactate content. In summary, the addition of 654 mg/L calcium lactate to the water improved the survival, growth performance, calcium deposition in tissues, and expression of molting-related genes in juvenile E. sinensis, as well as reduced the limb loss rate after each molting.

    参考文献
    [1] WANG H N, JIANG X D, WU X G, et al. The comparison of edible rate and biochemical composition of the second-year early-maturing and late-maturing strains of the third selective, generation during the adult culture of Chinese mitten crab Eriocheir sinensis[J]. Journal of Shanghai Ocean University, 2018, 27(4):487-493.王海宁,姜晓东,吴旭干,等.中华绒螯蟹二龄早熟和晚熟品系第三代成蟹可食率和生化组成的比较研究[J].上海海洋大学学报, 2018, 27(4):487-493.
    [2] WANG S H, ZHAO J S, WU X G, et al. Growth performance, gonadal development and nutritional composition of adult Eriocheir sinensis from Dongying[J]. Journal of Shanghai Ocean University, 2020, 29(1):17-26.王世会,赵金山,吴旭干,等.东营池塘养殖中华绒螯蟹生长性能、性腺发育及营养品质[J].上海海洋大学学报, 2020, 29(1):17-26.
    [3] Bureau of Fisheries, Ministry of Agriculture and Rural Affairs, National Fisheries Technology Extension Center, China Society of Fisheries. 2023 China fishery statistical yearbook[M]. Beijing:China Agriculture Press, 2023.农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴-2023[M].北京:中国农业出版社, 2023.
    [4] GU Z M, HE L G. Histological and cytological observation on the development cycle of crab (Eriocheir sinensis) ovary[J]. Oceanologia et Limnologia Sinica, 1997, 28(2):138-145.顾志敏,何林岗.中华绒螯蟹卵巢发育周期的组织学细胞学观察[J].海洋与湖沼, 1997, 28(2):138-145.
    [5] GAO Y, ZHANG X J, WEI J K, et al. Whole transcriptome analysis provides insights into molecular mechanisms for molting in Litopenaeus vannamei[J]. PLoS One, 2015, 10(12):e0144350.
    [6] ZHANG C, YANG X Z, XU M J, et al. Melatonin promotes cheliped regeneration, digestive enzyme function, and immunity following autotomy in the Chinese mitten crab, Eriocheir sinensis[J]. Frontiers in Physiology, 2018, 9:269.
    [7] HE J, WU X G, CHENG Y X. Effects of limb autotomy on growth, feeding and regeneration in the juvenile Eriocheir sinensis[J]. Aquaculture, 2016, 457:79-84.
    [8] CHEN S Q, CHEN Y, ZHOU G Q, et al. Effects of ecdysterone on the molt and growth of Procambarus clarkii[J]. Journal of Nanjing Normal University (Natural Science Edition), 2012, 35(1):80-83.陈树桥,陈勇,周国勤,等.蜕皮激素对克氏原螯虾蜕皮和生长的影响[J].南京师大学报(自然科学版), 2012, 35(1):80-83.
    [9] AIZENBERG J, LAMBERT G, WEINER S, et al. Factors involved in the formation of amorphous and crystalline calcium carbonate:a study of an ascidian skeleton[J]. Journal of the American Chemical Society, 2002, 124(1):32-39.
    [10] WHEATLY M G, ZANOTTO F P, HUBBARD M G. Calcium homeostasis in crustaceans:subcellular Ca dynamics[J]. Comparative Biochemistry and Physiology, Part B:Biochemistry and Molecular Biology, 2002, 132(1):163-178.
    [11] YANG H, YANG Z G, ZHANG L. Changes in calcium content, histological structure and expression of calreticulin gene of Chinese mitten crab Eriocheir sinensis during molting cycle[J]. Fisheries Science, 2022, 41(1):116-121.杨航,杨志刚,张龙.蜕壳周期内中华绒螯蟹钙含量、组织结构及相关基因表达变化[J].水产科学, 2022, 41(1):116-121.
    [12] CALHOUN S, ZOU E M. Epidermal carbonic anhydrase activity and exoskeletal metal content during the molting cycle of the blue crab, Callinectes sapidus[J]. Journal of Experimental Zoology Part A:Ecological and Integrative Physiology, 2016, 325(3):200-208.
    [13] YANG H, YANG Z G, ZHANG L, et al. Changes of calcium content and tissue structure in gill and carapaces of juvenile Eriocheir sinensis during the molting cycle[J]. Journal of Shanghai Ocean University, 2021, 30(3):484-491.杨航,杨志刚,张龙,等.中华绒螯蟹幼蟹在蜕壳周期内鳃和外壳的钙含量及组织结构变化[J].上海海洋大学学报, 2021, 30(3):484-491.
    [14] PAN J. Study on the expression of vitellogenesis-related genes regulated by two steroids in Eriocheir sinensis[D]. Shanghai:Shanghai Ocean University, 2020.潘杰.两种类固醇对中华绒螯蟹卵黄蛋白原及其受体基因的表达调控[D].上海:上海海洋大学, 2020.
    [15] WANG Y, YANG Z G, GUO Z H, et al. The full length cDNA cloning and expression analysis of RXR from the Chinese mitten crab (Eriocheir sinensis)[J]. Journal of Fisheries of China, 2013, 37(12):1761-1769.王瑶,杨志刚,郭子好,等.中华绒螯蟹RXR基因全长cDNA克隆及表达分析[J].水产学报, 2013, 37(12):1761-1769.
    [16] BERRIDGE M J, BOOTMAN M D, RODERICK H L. Calcium signalling:dynamics, homeostasis and remodelling[J]. Nature Reviews Molecular Cell Biology, 2003, 4(7):517-529.
    [17] WANG M L, ZHANG X M, HUANG G Q, et al. Effects of Ca2+ and Mg2+ concentrations on growth, SOD and CAT enzymatic activity of juvenile Paralichthys olivaceus[J]. Progress in Fishery Sciences, 2010, 31(3):29-36.王茂林,张秀梅,黄国强,等.不同钙、镁浓度对褐牙鲆幼鱼生长及SOD和CAT酶活力的影响[J].渔业科学进展, 2010, 31(3):29-36.
    [18] GAO W H, XU L, TIAN J, et al. The research progress on calcium and phosphorus requirements in aquatic animals[J]. Freshwater Fisheries, 2023, 53(2):104-112.郜卫华,徐磊,田娟,等.水产动物对钙、磷需要量的研究进展[J].淡水渔业, 2023, 53(2):104-112.
    [19] HOU C Q. Effects and mechanism of Ca2+ concentration on the molting and growth of juvenile Litopenaeus vannamei[D]. Qingdao:Ocean University of China, 2011.侯纯强.水中钙浓度对凡纳滨对虾(Litopenaeus vannamei)蜕皮和生长的影响及生理生态学机制[D].青岛:中国海洋大学, 2011.
    [20] PÉREZ-FUENTETAJA A, GOODBERRY F. Daphnia's challenge:survival and reproduction when calcium and food are limiting[J]. Journal of Plankton Research, 2016, 38(6):1379-1388.
    [21] HAMMOND K S, HOLLOWS J W, TOWNSEND C R, et al. Effects of temperature and water calcium concentration on growth, survival and moulting of freshwater crayfish, Paranephrops zealandicus[J]. Aquaculture, 2006, 251(2/4):271-279.
    [22] TIAN Z H, KANG X J, JIAO C Z. Structural and constituent changes in integument during the molt cycle of Chinese mitten crab Eriocheir sinensis[J]. Acta Hydrobiologica Sinica, 2013, 37(5):899-904.田志环,康现江,焦传珍.中华绒螯蟹蜕皮过程中体壁结构和主要成分的变化[J].水生生物学报, 2013, 37(5):899-904.
    [23] ZHAO L, LONG X W, WU X G, et al. Effects of water salinity on osmoregulation and physiological metabolism of adult male Chinese mitten crab Eriocheir sinensis[J]. Acta Hydrobiologica Sinica, 2016, 40(1):27-34.赵磊,龙晓文,吴旭干,等.水体盐度对中华绒螯蟹成体雄蟹渗透压调节和生理代谢的影响[J].水生生物学报, 2016, 40(1):27-34.
    [24] DONG S L, DU F S, LAI W. Effects of pH and Ca2+ concentration on growth and energy budget of Macrobrachium nipponense[J]. Journal of Fisheries of China, 1994, 18(2):118-123.董双林,堵甫山,赖伟. pH值和Ca2+浓度对日本沼虾生长和能量收支的影响[J].水产学报, 1994, 18(2):118-123.
    [25] CHEN J. Observation on individual molting, growth and analysis of relative gene expression in Chinese mitten crab[D]. Shanghai:Shanghai Ocean University, 2016.陈娇.中华绒螯蟹蜕壳、生长的个体观察和相关基因的表达分析[D].上海:上海海洋大学, 2016.
    [26] SLOS S, DE BLOCK R, STOKS R. Autotomy reduces immune function and antioxidant defence[J]. Biology Letters, 2009, 5(1):90-92.
    [27] HUANG S. Study on reciprocal regulation mechanism of MIH and EcR genes in the molting process of Chinese mitten crab, Eriocheir sinensis[D]. Shanghai:Shanghai Ocean University, 2017.黄姝. EcRMIH基因在中华绒螯蟹蜕壳过程中的相互调节机制研究[D].上海:上海海洋大学, 2017.
    [28] DURICA D S, CHUNG A C K, HOPKINS P M. Characterization of EcR and RXR gene homologs and receptor expression during the molt cycle in the crab, Uca pugilator[J]. American Zoologist, 1999, 39(4):758-773.
    [29] PROMWIKORN W, BOONYOUNG P, KIRIRAT P. Histological characterization of cuticular depositions throughout the molting cycle of the black tiger shrimp (Penaeus monodon)[J]. Songklanakarin Journal of Science & Technology, 2005, 27(3):499-509.
    [30] AHEARN G A, MANDAL P K, MANDAL A. Calcium regulation in crustaceans during the molt cycle:a review and update[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2004, 137(2):247-257.
    [31] LONG X W, WU X G, ZHAO L, et al. Effects of salinity on gonadal development, osmoregulation and metabolism of adult male Chinese mitten crab, Eriocheir sinensis[J]. PLoS One, 2017, 12(6):e0179036.
    [32] SOWERS A D, YOUNG S P, GROSELL M, et al. Hemolymph osmolality and cation concentrations in Litopenaeus vannamei during exposure to artificial sea salt or a mixed-ion solution:relationship to potassium flux[J]. Comparative Biochemistry and Physiology, Part A:Molecular & Integrative Physiology, 2006, 145(2):176-180.
    [33] WILDER M N, IKUTA K, ATMOMARSONO M, et al. Changes in osmotic and ionic concentrations in the hemolymph of Macrobrachium rosenbergii exposed to varying salinities and correlation to ionic and crystalline composition of the cuticle[J]. Comparative Biochemistry & Physiology Part A:Molecular & Integrative Physiology, 1998, 119(4):941-950.
    [34] QI C L, PU X Y, ZHENG J J, et al. Correlation between hepatopancreas lipid metabolism and calcium during exoskeleton mineralization in Eriocheir sinensis[J]. Journal of Fisheries of China, 2022, 46(10):2007-2015.戚常乐,卜宪勇,郑佳君,等.中华绒螯蟹外骨骼矿化期肝胰腺脂代谢与钙离子的相关性[J].水产学报, 2022, 46(10):2007-2015.
    [35] XU B P. The functional study of the irons transport related molecules in Scylla paramamosain during the molt cycle[D]. Hangzhou:Zhejiang University, 2016.徐宾朋.拟穴青蟹蜕皮周期中离子转运相关基因的研究[D].杭州:浙江大学, 2016.
    [36] PERRY H, TRIGG C, LARSEN K, et al. Calcium concentration in seawater and exoskeletal calcification in the blue crab, Callinectes sapidus[J]. Aquaculture, 2001, 198(3/4):197-208.
    [37] DONG S S, DONG S L, WANG F, et al. The effect of Ca2+ concentration on the growth of juvenile Litopenaeus vannamei[J]. Journal of Fisheries of China, 2005, 29(2):211-215.董少帅,董双林,王芳,等. Ca2+浓度对凡纳滨对虾稚虾生长的影响[J].水产学报, 2005, 29(2):211-215.
    [38] YANG F Y, WEN B L, LI X Y, et al. Investigation of water environment and fish diversity in dalinor wetlands Ⅱ. alkalinity, pH and hardness of the water of Dali lake[J]. Wetland Science, 2020, 18(6):646-652.杨富亿,文波龙,李晓宇,等.达里诺尔湿地水环境和鱼类多样性调查Ⅱ.达里湖水体的碱度、pH和硬度[J].湿地科学, 2020, 18(6):646-652.
    [39] LU J, ZHUANG P, FENG G P, et al. Response of osmoregulation and antioxidation system to water salinity in Parent Chinese mitten crab (Eriocheir sinensis)[J]. Marine Fisheries, 2011, 33(1):39-45.卢俊,庄平,冯广朋,等.中华绒螯蟹亲蟹渗透压调节和抗氧化系统对盐度的响应[J].海洋渔业, 2011, 33(1):39-45.
    [40] YANG Y H, LI M S, LUO L, et al. Study on toxicity of salinity and alkalinity on Eriocheir sinensis[J]. Journal of Northeast Agricultural University, 2022, 53(2):36-41.杨雨虹,李明帅,罗亮,等.盐碱胁迫对中华绒鳌蟹毒性作用研究[J].东北农业大学学报, 2022, 53(2):36-41.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

黄俊杰,朱筛成,马天利,蒋栋梁,成永旭.水体中添加乳酸钙对中华绒螯蟹生长、蜕壳、钙沉积和水质指标的影响[J].上海海洋大学学报,2024,33(5):1106-1119.
HUANG Junjie, ZHU Shaicheng, MA Tianli, JIANG Dongliang, CHENG Yongxu. Effects of adding calcium lactate to water on growth, molting, calcium deposition of Chinese mitten crab and water quality[J]. Journal of Shanghai Ocean University,2024,33(5):1106-1119.

复制
分享
文章指标
  • 点击次数:186
  • 下载次数: 2567
  • HTML阅读次数: 141
  • 引用次数: 0
历史
  • 收稿日期:2023-11-16
  • 最后修改日期:2024-03-09
  • 在线发布日期: 2024-09-05
文章二维码