克氏原螯虾三群体双列杂交组合生长性能和耐干露能力比较分析
CSTR:
作者:
基金项目:

上海市科技创新行动计划项目(23N11900300);安徽省水产种业振兴项目(2022AHNYNC081FW)


Comparative analysis of growth performance and desiccation resistance of three-population diallel hybrid combination of Procambarus clarkii
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [39]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    以遗传多样性高、生长快的江苏滆湖、江苏建湖和安徽芜湖3个克氏原螯虾优异种质群体进行双列杂交试验,得到6个杂交组[滆湖♀×建湖(GJ)、建湖♀×滆湖(JG)、滆湖♀×芜湖(GW)、芜湖♀×滆湖(WG)、建湖♀×芜湖(JW)、芜湖♀×建湖♂(WJ)]和3个自交组[滆湖♀×滆湖(GG)、建湖♀×建湖(JJ)、芜湖♀×芜湖(WW)],对9个组合生长性能和生态适应性比较分析,结果表明:(1)养殖周期内,WW和JJ组合雌雄个体体质量特定增长率、增重率均显著大于其余组合;除GJ和JG外其余组合雄性个体体质量特定增长率、增重率显著大于雌性,3—5月各杂交组生长性状均未见杂种优势;(2)随养殖时间的增加,9个群体克氏原螯虾头胸甲长/体长、腹部长/体长变化明显,头胸甲长/体长逐渐增加,腹部长/体长逐渐减小且5月雌雄个体间出现分化,雌性腹部长占比显著大于雄性。头胸甲宽/体长、腹部宽/体长、尾节长/体长仅4月有明显增加;(3)干露24 h各组虾开始出现死亡,48 h各组存活率出现显著分化,48 h时WW组合存活率显著大于其余组合,GJ和JG两个正反交组在耐干露能力方面呈现出杂种优势。本研究发现克氏原螯虾种质混杂一定程度限制了生长性状杂种优势,但抗逆性表现出杂种优势,适当杂交再选育是重要的育种策略,克氏原螯虾生长和腹部长占比呈明显雌雄二态性,单性育种也是重要方向。

    Abstract:

    This study conducted diallel hybridization experiments on three excellent populations of Procambarus clarkii, namely Gehu, Jiangsu; Jianhu, Jiangsu; and Wuhu, Anhui, with high genetic diversity and fast growth. Six hybrid groups were obtained[Gehu ♀×Jianhu (GJ), Jianhu ♀×Gehu (JG), Gehu ♀×Wuhu (GW), Wuhu ♀×Gehu (WG), Jianhu ♀×Wuhu (JW), Wuhu ×Jianhu (WJ)], and three self crossing groups[Gehu ♀×Gehu (GG), Jianhu ♀×Jianhu ♂ (JJ), Wuhu ×Wuhu (WW)]. A comparative analysis was conducted on the growth performance and ecological adaptability of 9 combinations. The results showed that:(1) During the breeding cycle, the weight gain and weight gain rate of both male and female individuals in the WW and JJ combinations were significantly higher than those in the other combinations (P<0.05); Except for GJ and JG, the weight gain and growth rate of male individuals in all combinations were significantly higher than those in females. From March to May, no heterosis was observed in the growth traits of each hybrid group; (2) With the increase of breeding time, there were significant changes in the length/body length of the head and chest armor, as well as the length/body length of the abdomen in the 9 populations of crayfish. The length/body length of the head and chest armor gradually increased, while the length/body length of the abdomen gradually decreased. In May, there was differentiation between male and female individuals, and the proportion of female abdomen length was significantly higher than that of male. The ratio of head to chest armor width/body length, abdominal width/body length, and tail segment length/body length only showed a significant increase in April; (3) After 24 hours of dry dew, each group of shrimp began to die, and at 48 hours, the survival rate of each group showed significant differentiation. At 48 hours, the survival rate of the WW combination was significantly higher than that of the other combinations. The GJ and JG cross groups showed heterosis in terms of dry dew tolerance. This study found that the mixed germplasm of crayfish limited the heterosis of growth traits to a certain extent, but showed heterosis in stress resistance. Proper hybridization and re-selection are important breeding strategies. The growth and abdominal length ratio of crayfish showed obvious male and female dimorphism, and single sex breeding is also an important direction for future research.

    参考文献
    [1] FARRAG M M S, EL-GEDDAWY M A M A, AHMED Z S A. More evidences for the nutritional quality and future exploitation of the invasive crayfish Procambarus clarkii (Girard, 1852) from the River Nile, Egypt[J]. The Egyptian Journal of Aquatic Research, 2022, 48(2):151-156.
    [2] HE M D, LIU F, WANG F. Resource utilization, competition and cannibalism of the red swamp crayfish Procambarus clarkii in integrated rice-crayfish culture without artificial diets[J]. Aquaculture Reports, 2021, 20:100644.
    [3] HENTTONEN P, HUNER J V. The introduction of alien species of crayfish in Europe:a historical introduction[M]//GHERARDI F. Crayfish in Europe as Alien Species. London:Routledge, 2017:13-22.
    [4] XI Y W. Discussion on the development value of Procambarus clarkii[J]. Journal of Beijing Fisheries, 2002(4):8-9. 奚业文. 浅谈克氏原螯虾的开发价值[J]. 北京水产, 2002(4):8-9.
    [5] SONG L, ZHANG J P, HAN X L, et al. Analysis of breeding situation and countermeasures of Procambarus clarkia[J]. Journal of Changshu Institute Technology (Natural Sciences), 2011, 25(2):85-87, 91. 宋亮, 张建平, 韩晓磊, 等. 克氏原螯虾养殖现状及对策[J]. 常熟理工学院学报(自然科学), 2011, 25(2):85-87, 91.
    [6] LOU Y D. Close hybridization of fish and its application in aquaculture[J]. Journal of Fisheries of China, 2007, 31(4):532-538. 楼允东. 我国鱼类近缘杂交研究及其在水产养殖上的应用[J]. 水产学报, 2007, 31(4):532-538.
    [7] WU Y P, CHEN A H, ZHANG Y, et al. Heterosis analysis and diallel cross of red Meretrix meretrix[J]. Oceans and Lakes, 2015, 46(1):43-49. 吴杨平, 陈爱华, 张雨, 等. 红壳文蛤双列杂交及杂种优势分析[J]. 海洋与湖沼, 2015, 46(1):43-49.
    [8] JIANG Z B, DAI X L, MING L, et al. Analysis of intraspecific heterosis, heritability and genetic correlation of growth traits of Macrobrachium rosenbergii[J]. Journal of Shanghai Ocean University, 2017, 26(2):189-196. 江宗冰, 戴习林, 明磊, 等. 罗氏沼虾生长性状的种内杂交优势及遗传力与遗传相关分析[J]. 上海海洋大学学报, 2017, 26(2):189-196.
    [9] ZHOU J S, CAO Z M, YANG G L, et al. Study on hybrid of Burma introduced and Zhejiang locally-cultured populations of giant prawn Macrobrachium rosenbergii de man and SRAP marker[J]. Journal of Fishery Sciences of China, 2006, 13(4):667-673. 周劲松, 曹哲明, 杨国梁, 等. 罗氏沼虾缅甸引进种和浙江本地种及其杂交种的生长性能与SRAP分析[J]. 中国水产科学, 2006, 13(4):667-673.
    [10] ZHOU F L, YANG Q B, JIANG S, et al. Analysis of combining ability and heterosis on body mass trait of three Penaeusmonodon populations[J]. South China Fisheries Science, 2021, 17(1):39-44. 周发林, 杨其彬, 姜松, 等. 斑节对虾3个种质群体体质量性状配合力及杂种优势分析[J]. 南方水产科学, 2021, 17(1):39-44.
    [11] BARBARESI S, FANI R, GHERARDI F, et al. Genetic variability in European populations of an invasive American crayfish:preliminary results[J]. Biological Invasions, 2003, 5(3):269-274.
    [12] BARBARESI S, GHERARDI F, MENGONI A, et al. Genetics and invasion biology in fresh waters:a pilot study of Procambarus clarkii in Europe[M]//GHERARDI F. Biological Invaders in Inland Waters:Profiles, Distribution, and Threats. Dordrecht:Springer, 2007:381-400.
    [13] BARKI A, LEVI T, HULATA G, et al. Annual cycle of spawning and molting in the red-claw crayfish, Cherax quadricarinatus, under laboratory conditions[J]. Aquaculture, 1997, 157(3/4):239-249.
    [14] ZHOU Z X, FAN W H, FANG L, et al. Effects of temperature on the growth, feeding, and digestive enzyme activity of the juvenile red swamp crayfish (Procambarus clarkii)[J]. Hebei Agricultural Sciences, 2021, 60(20):127-130. 周泽湘, 范文浩, 方刘, 等. 温度对克氏原螯虾幼虾生长、摄食及消化酶活性的影响[J]. 湖北农业科学, 2021, 60(20):127-130.
    [15] HU Y C. Analysis on half-sib families of Procambrus alarkii and the parentage assignment using microsatellite markers[D]. Nanjing:Nanjing Agricultural University, 2015. 胡亚成. 微卫星标记应用于克氏原螯虾的半同胞家系分析和亲子鉴定[D]. 南京:南京农业大学, 2015.
    [16] LI H P. Studies on sex-related molecular markers of river prawn (Macrobrachium nipponense)[D]. Nanjing:Nanjing Agricultural University, 2009. 李慧萍. 青虾性别相关分子标记的研究[D]. 南京:南京农业大学, 2009.
    [17] YU Y Q. The molecular characterization and functional analysis of sexual development related genes Sxl and Dmrt in the prawn, Macrobrachium rosenbergii[D]. Hangzhou:Zhejiang University, 2014. 俞炎琴. 罗氏沼虾中性别发育相关基因SxlDmrt基因的分子特征和功能研究[D]. 杭州:浙江大学, 2014.
    [18] XIAO G X, SONG W P, GUO B, et al. Growth characteristics of Chinese shrimp (Fenneropenaeus chinensis) in Bohai Bay[J]. Marine Fisheries, 2014, 36(2):116-122. 肖广侠, 宋文平, 郭彪, 等. 渤海湾中国明对虾的生长特性[J]. 海洋渔业, 2014, 36(2):116-122.
    [19] HUANG J H, YANG Q B, MA Z M, et al. The growth, development and sexual maturity of pond-reared Penaeus monodon[J]. Journal of Fisheries of China, 2013, 37(3):397-406. 黄建华, 杨其彬, 马之明, 等. 池塘养殖斑节对虾生长、发育与性成熟[J]. 水产学报, 2013, 37(3):397-406.
    [20] YAN W H, HUANG C, TANG J Q, et al. Summary of contrast culture experiment of Procambarus clarkii in male and female ponds[J]. Journal of Aquaculture, 2015, 36(2):15-16. 严维辉, 黄成, 唐建清, 等. 克氏原螯虾雌雄分池对比养殖试验总结[J]. 水产养殖, 2015, 36(2):15-16.
    [21] HUANG G W, SHE M, ZHOU J S, et al. Nutritional analysis and evaluation of different parts of Procambarus clarkia with different size and gender[J]. Journal of Food Safety and Quality, 2023, 14(11):33-42. 黄国威, 佘敏, 周际松, 等. 不同规格及性别的克氏原螯虾各部位营养分析及评价[J]. 食品安全质量检测学报, 2023, 14(11):33-42.
    [22] LUO J P, LIU P M, GAO Q X. Research progress in selective breeding of Macrobrachium rosenbergii[J]. Biological Resources, 2023, 45(1):16-27. 罗金萍, 刘培敏, 高权新. 罗氏沼虾良种选育研究进展[J]. 生物资源, 2023, 45(1):16-27.
    [23] XIAO F, LIU Z S, GUO Y F, et al. China crayfish industry development report (2017)[J]. China Fisheries, 2017(7):8-17. 肖放, 刘忠松, 郭云峰, 等. 中国小龙虾产业发展报告(2017)[J]. 中国水产, 2017(7):8-17.
    [24] CUI W T, ZOU Y F, BAI Z Y, et al. Genetic diversity and structure analysis of Procambarus clarkii populations in Anhui Province[J]. Journal of Fisheries of China, 2023, 47(9):90-99. 崔文涛, 邹宇凡, 白志毅, 等. 安徽地区克氏原螯虾群体的遗传多样性和遗传结构[J]. 水产学报, 2023, 47(9):90-99.
    [25] XING Z J, JIANG H C, LU W, et al. Genetic diversity analysis of eight Procambarus clarkii stocks in Jiangsu Province based on microsatellites[J]. Journal of Shanghai Ocean University, 2014, 23(5):656-662. 邢智珺, 姜虎成, 陆伟, 等. 江苏8个克氏原螯虾群体遗传多样性微卫星分析[J]. 上海海洋大学学报, 2014, 23(5):656-662.
    [26] AN D D. Studies on germplasm evaluation and utilization of cultured populations of Procambarus clarkii in Guizhou[D]. Guiyang:Guizhou University, 2021. 安丹丹. 贵州克氏原螯虾养殖群体种质评价及利用研究[D]. 贵阳:贵州大学, 2021.
    [27] HUANG X F, TANG Z S, LIU J D, et al. Genetic diversity microsatellite analysis of Procambarus clarkii populations in different regions of Guangxi[J]. Journal of Southern Agriculture, 2020, 51(2):437-444. 黄小芳, 唐章生, 刘俊丹, 等. 广西不同地区克氏原螯虾群体遗传多样性微卫星分析[J]. 南方农业学报, 2020, 51(2):437-444.
    [28] TAN Y F, PENG G H, XIONG L J, et al. Genetic diversity and structure analysis of 13 red swamp crayfish (Procambarus clarkii) populations in Yangtze River basin[J]. Journal of Huazhong Agricultural University, 2020, 39(2):33-39. 谭云飞, 蓬国辉, 熊礼静, 等. 长江中下游流域13个克氏原螯虾群体遗传多样性和遗传结构分析[J]. 华中农业大学学报, 2020, 39(2):33-39.
    [29] TIAN Y, KONG J, YANG C H. Comparison of the growth and survival rate of the first generation of hybrid offspring from two populations of Chinese shrimp[J]. Chinese Science Bulletin, 2006(15):1771-1775. 田燚, 孔杰, 杨翠华. 中国对虾2个群体杂交子一代生长和存活率比较[J]. 科学通报, 2006, 51(15):1771-1775.
    [30] LIN H J, SHEN Q, ZHANG L P, et al. A diallel analysis on growth traits in shrimp Litopenaeus vannamei[J]. Journal of Tropical Oceanography, 2010, 29(6):51-56. 林红军, 沈琪, 张吕平, 等. 凡纳滨对虾生长性状的双列杂交分析[J]. 热带海洋学报, 2010, 29(6):51-56.
    [31] ZHANG G F, LIU X, QUE H Y, et al. The theory and application of hybridization and heterosis in marine mollusks[J]. Marine Sciences, 2004, 28(7):54-60. 张国范, 刘晓, 阙华勇, 等. 贝类杂交及杂种优势理论和技术研究进展[J]. 海洋科学, 2004, 28(7):54-60.
    [32] LI T G. The effects of different salinity on survival and growth and Metabolism performance and embryonic develoment of Protostellus clarkii[D]. Qingdao:Ocean University of China, 2007. 李庭古. 盐度对克氏原螯虾的存活、生长、代谢及受精卵孵化的影响[D]. 青岛:中国海洋大学, 2007.
    [33] FAN W H, FANG L, ZHOU J, et al. Effects of stocking density on growth, digestive enzyme and immune enzyme activities of red swamp crayfish Procambarus clarkii[J]. Fisheries Science, 2021, 40(2):261-266. 范文浩, 方刘, 周锦, 等. 养殖密度对克氏原螯虾生长及消化酶、免疫酶活性的影响[J]. 水产科学, 2021, 40(2):261-266.
    [34] YANG W X, CHEN X R, WEN H, et al. Effects of dietary protease supplementation in high-plant-protein diets on the growth, immunity and digestion of red swamp crayfish (Procambarus clarkii)[J]. Journal of Fisheries of China, 2022, 46(6):1053-1062. 杨文秀, 陈效儒, 文华, 等. 高植物蛋白饲料中添加蛋白酶对克氏原螯虾生长、免疫力及消化力的影响[J]. 水产学报, 2022, 46(6):1053-1062.
    [35] DENG H F. Effects of different light and diet on growth, non-specific immune enzymes and body composition of the crayfish, Procambarus clarkii[D]. Jingzhou:Yangtze University, 2018. 邓慧芳. 不同光照和饲料对克氏原螯虾生长、非特异性免疫酶及体成分的影响[D]. 荆州:长江大学, 2018.
    [36] FANG H. Selection of shelter for crayfish (Procambarus clarkii) and the effect of shelter on crayfish[D]. Nanjing:Nanjing University, 2019. 方卉. 克氏原螯虾对隐蔽所的选择及其对隐蔽所的生长适应性[D]. 南京:南京大学, 2019.
    [37] WANG Y Y, CHENG Y X, LI C L, et al. Effects of desiccation on survival, metabolism-related enzymes and histological structure of adult red swamp crayfish (Procambarus clarkii)[J]. South China Fisheries Science, 2021, 17(5):34-44. 王源源, 成永旭, 李晨露, 等. 干露对克氏原螯虾成虾存活、相关代谢酶及组织结构的影响[J]. 南方水产科学, 2021, 17(5):34-44.
    [38] OMORI K, IRAWAN B, KIKUTANI Y. Studies on the salinity and desiccation tolerances of Helice tridens and Helice japonica (Decapoda:Grapsidae)[J]. Hydrobiologia, 1998, 386(1/3):27-36.
    [39] JIANG N, WANG F, LU Y L, et al. Effects of air-exposure stress on antioxidant capacity and stress response of swimming crab (Portunus trituberculatus)[J]. Journal of Fisheries of China, 2014, 38(12):1996-2004. 姜娜, 王芳, 路允良, 等. 干露对三疣梭子蟹抗氧化和应激能力的影响[J]. 水产学报, 2014, 38(12):1996-2004.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

邹宇凡,吴玮杰,白志毅,李典中,蒋军,程熙,李家乐.克氏原螯虾三群体双列杂交组合生长性能和耐干露能力比较分析[J].上海海洋大学学报,2024,33(2):361-370.
ZOU Yufan, WU Weijie, BAI Zhiyi, LI Dianzhong, JIANG Jun, CHENG Xi, LI Jiale. Comparative analysis of growth performance and desiccation resistance of three-population diallel hybrid combination of Procambarus clarkii[J]. Journal of Shanghai Ocean University,2024,33(2):361-370.

复制
分享
文章指标
  • 点击次数:287
  • 下载次数: 669
  • HTML阅读次数: 24
  • 引用次数: 0
历史
  • 收稿日期:2023-09-12
  • 最后修改日期:2023-12-18
  • 录用日期:2023-12-20
  • 在线发布日期: 2024-03-19
文章二维码